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GEO Series: GSE113186

Title: The role of maternal pioneer factors in predefining first zygotic responses to inductive signals

Summary

Embryonic development yields many different cell types in response to just a few families of inductive signals. The property of a signal-receiving cell that determines how it responds to such signals, including the activation of cell type-specific genes, is known as its competence. Here, we show how maternal factors modify chromatin to specify initial competence in the frog Xenopus tropicalis. We identified the earliest engaged regulatory DNA sequences, and inferred from them critical activators of the zygotic genome. Of these, we showed that the pioneering activity of the maternal pluripotency factors Pou5f3 and Sox3 predefine competence for germ layer formation by extensively remodeling compacted chromatin before the onset of signaling. The remodeling includes the opening and marking of thousands of regulatory elements, extensive chromatin looping, and the co-recruitment of signal-mediating transcription factors. Our work identifies significant developmental principles that inform our understanding of how pluripotent stem cells interpret inductive signals.


Contributors: George Gentsch, George Gentsch, James Smith

Experiment Type: This study generated 202 samples of different applications of high-throughput sequencing inluding ChIP-Seq, DNase-Seq, next-generation capture-C and RNA-Seq.

Experiment Reagents: 1062368-24-4, alpha-amanitin, ctnnb1 MO2,

Phenotypes:
WT + alpha-amanitin + NF10 (RNA-Seq)

Article: XB-ART-56302, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
WE - NF8 MBT (NG Cap-C) GSM3099522  GSM3099523  GSM3099524  Hi-C
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT (NG Cap-C) GSM3099525  GSM3099526  GSM3099527  Hi-C
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE - NF8 MBT naked (DNase-Seq) GSM3099520  GSM3099521  DNase-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT (DNase-Seq) GSM3099518  GSM3099519  DNase-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE - NF8 MBT (DNase-Seq) GSM3099516  GSM3099517  DNase-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE + DMSO - NF10 GSM3099597  GSM3099598  GSM3099599  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + LDN193189 - NF10 DEG GSM3099588  GSM3099589  GSM3099590  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical 1062368-24-4 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + SB431542 - NF10 DEG GSM3099579  GSM3099580  GSM3099581  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical SB431542 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
WE + DMSO - NF8 MBT GSM3099591  GSM3099592  GSM3099593  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + LDN193189 - NF8 MBT DEG GSM3099582  GSM3099583  GSM3099584  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical 1062368-24-4 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + SB431542 - NF8 MBT DEG GSM3099573  GSM3099574  GSM3099575  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical SB431542 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE + DMSO - NF9 GSM3099594  GSM3099595  GSM3099596  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + LDN193189 - NF9 DEG GSM3099585  GSM3099586  GSM3099587  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical 1062368-24-4 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + SB431542 - NF9 DEG GSM3099576  GSM3099577  GSM3099578  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical SB431542 NF stage 4 (8-cell) embryo
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
input WE - NF10 GSM3099505  GSM3099506  GSM3099507  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   Ctnnb1 WE - NF10 GSM3099461  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Ctnnb1 Ab2
   Myod1 WE + myod1 - NF10 GSM3099476  GSM3099477  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(HA-myod1) myod1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 HA Ab11
   Pol II WE + myod1 - NF10 GSM3099480  GSM3099481  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(HA-myod1) myod1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Polr2a Ab2
   Smad2/3 WE - NF10 GSM3099466  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Smad2/3 Ab1
   VegT WE - NF10 GSM3099474  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 VegT Ab1
   phospho-smad1/5/9 WE - NF10 GSM3099463  GSM3099464  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Smad1 Ab7
   sox3 WE + myod1 - NF10 GSM3099478  GSM3099479  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(HA-myod1) myod1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Sox3 Ab2
   sox3 WE - NF10 GSM3099468  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10 Sox3 Ab2
input WE - NF11 GSM3099508  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11  
   Pol II WE - NF11 GSM3099456  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11 Polr2a Ab2
input WE - NF12 GSM3099509  GSM3099510  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 12  
   Pol II WE - NF12 GSM3099457  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 12 Polr2a Ab2
   sox3 WE - NF12 GSM3099469  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 12 Sox3 Ab2
input WE - NF13 GSM3099511  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 13  
   Pol II WE - NF13 GSM3099458  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 13 Polr2a Ab2
input WE - NF20 GSM3099512  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 20  
   Tbx6 WE - NF20 GSM3099475  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 20 Tbx6 Ab1
input tail bud - NF20 GSM3099515  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
tail bud NF stage 20  
   Sox3 tail bud - NF20 GSM3099472  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
tail bud NF stage 20 Sox3 Ab2
input head - NF20 GSM3099513  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
head NF stage 20  
   Sox3 head - NF20 GSM3099470  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
head NF stage 20 Sox3 Ab2
input trunk - NF20 GSM3099514  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
trunk NF stage 20  
   Sox3 trunk - NF20 GSM3099471  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
trunk NF stage 20 Sox3 Ab2
input WE - NF6 GSM3099498  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 6 (32-cell)  
   Ctnnb1 WE - NF6 GSM3099459  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 6 (32-cell) Ctnnb1 Ab2
   Pol II WE - NF6 GSM3099452  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 6 (32-cell) Polr2a Ab2
input WE - NF7 GSM3099499  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 7  
   Pol II WE - NF7 GSM3099453  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 7 Polr2a Ab2
input WE - NF8 GSM3099500  GSM3099501  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   Ctnnb1 WE - NF8 GSM3099460  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Ctnnb1 Ab2
   Pol II WE - NF8 GSM3099454  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Polr2a Ab2
   Smad2/3 WE - NF8 GSM3099465  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Smad2/3 Ab1
   Sox3 WE - NF8 GSM3099467  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Sox3 Ab2
   VegT WE - NF8 GSM3099473  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 VegT Ab1
input WE - NF8 MBT GSM3099502  GSM3099503  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   H3K4me1 + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT GSM3099496  GSM3099497  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 methyl-H3f3a Ab13
   H3K4me1 WE - NF8 MBT GSM3099494  GSM3099495  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 methyl-H3f3a Ab13
   Pol II WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT GSM3099492  GSM3099493  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Polr2a Ab2
   Pol II WE - NF8 MBT GSM3099455  GSM3099490  GSM3099491  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Polr2a Ab2
   Smad2/3 WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT GSM3099484  GSM3099485  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Smad2/3 Ab1
   Smad2/3 WE - NF8 MBT GSM3099482  GSM3099483  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Smad2/3 Ab1
   phospho-smad1/5/9 WE - NF8 MBT GSM3099462  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8 Smad1 Ab7
input WE - NF9 GSM3099504  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   Ctnnb1 WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF9 GSM3099488  GSM3099489  ChIP-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9 Ctnnb1 Ab2
   Ctnnb1 WE - NF9 GSM3099486  GSM3099487  ChIP-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9 Ctnnb1 Ab2
WE - NF10 GSM3099543  GSM3099544  GSM3099545  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + alpha-amanitin - NF10 DEG GSM3099534  GSM3099535  GSM3099536  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical alpha-amanitin NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
WE - NF8 MBT GSM3099537  GSM3099538  GSM3099539  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + alpha-amanitin - NF8 MBT DEG GSM3099528  GSM3099529  GSM3099530  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical alpha-amanitin NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE - NF9 GSM3099540  GSM3099541  GSM3099542  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + alpha-amanitin - NF9 DEG GSM3099531  GSM3099532  GSM3099533  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
chemical alpha-amanitin NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
WE - NF10 GSM3099570  GSM3099571  GSM3099572  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + ctnnb1 MO - NF10 DEG GSM3099552  GSM3099553  GSM3099554  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO2 ctnnb1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + hbg1 cMO - NF10 DEG GSM3099561  GSM3099562  GSM3099563  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
WE - NF8 MBT GSM3099564  GSM3099565  GSM3099566  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + ctnnb1 MO - NF8 MBT DEG GSM3099546  GSM3099547  GSM3099548  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO2 ctnnb1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + hbg1 cMO - NF8 MBT DEG GSM3099555  GSM3099556  GSM3099557  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE - NF9 GSM3099567  GSM3099568  GSM3099569  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + ctnnb1 MO - NF9 DEG GSM3099549  GSM3099550  GSM3099551  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO2 ctnnb1 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + hbg1 cMO - NF9 DEG GSM3099558  GSM3099559  GSM3099560  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
WE - NF10 GSM3099624  GSM3099625  GSM3099626  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF10 DEG GSM3099615  GSM3099616  GSM3099617  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + pou5f3.2 MO + pou5f3.3 MO - NF10 DEG GSM3099606  GSM3099607  GSM3099608  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
WE - NF8 MBT GSM3099618  GSM3099619  GSM3099620  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF8 MBT DEG GSM3099609  GSM3099610  GSM3099611  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + pou5f3.2 MO + pou5f3.3 MO - NF8 MBT DEG GSM3099600  GSM3099601  GSM3099602  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE - NF9 GSM3099621  GSM3099622  GSM3099623  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + pou5f3.2 MO + pou5f3.3 MO - NF9 DEG GSM3099603  GSM3099604  GSM3099605  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + pou5f3.2 MO + pou5f3.3 MO + sox3 MO - NF9 DEG GSM3099612  GSM3099613  GSM3099614  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pou5f3.2 MO7 pou5f3.2 NF stage 1 zygote
morpholino injection sox3 MO5 sox3 NF stage 1 zygote
morpholino injection pou5f3.3 MO6 pou5f3.3 NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
WE - NF10 GSM3099651  GSM3099652  GSM3099653  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + hbg1 cMO - NF10 DEG GSM3099642  GSM3099643  GSM3099644  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
   WE + vegt MO - NF10 DEG GSM3099633  GSM3099634  GSM3099635  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection vegt MO10 vegt NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 10  
WE - NF8 MBT GSM3099645  GSM3099646  GSM3099647  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + hbg1 cMO - NF8 MBT DEG GSM3099636  GSM3099637  GSM3099638  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
   WE + vegt MO - NF8 MBT DEG GSM3099627  GSM3099628  GSM3099629  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection vegt MO10 vegt NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 8  
WE - NF9 GSM3099648  GSM3099649  GSM3099650  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + hbg1 cMO - NF9 DEG GSM3099639  GSM3099640  GSM3099641  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
   WE + vegt MO - NF9 DEG GSM3099630  GSM3099631  GSM3099632  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection vegt MO10 vegt NF stage 1 zygote
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 9  
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