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GEO Series: GSE126058

Title: Identification of genes regulated by Erythroferrone (ERFE) in Xenopus embryos at tadpole stages.

Summary

RNA-seq was employed to analyze differential gene expression upon ERFE knockdown in Xenopus laevis embryos at three different developmental stages using two non-overlapping translation blocking morpholino oligonucleotides. Only a small number of genes was affected by both morpholinos and at more than one of the developmentel stages analyzed, among them gja4, the gene coding for Gap junction junction protein Alpha 4.


Contributors: Gabriela Salinas, Tomas Pieler, Juliane Melchert, Thomas Lingner

Experiment Type: Xenopus eight-cell stage embryos were injected with 10 ng ERFE-MO1 or 20 ng ERFE-MO2 into the four vegetal blastomeres and cultivated until stage 35/36, stage 37/38 or stage 41. Uninjected embryos as well as embryos injected with equal amounts of standard control morpholino (cMO) served as controls.

Experiment Reagents: erfe MO1, erfe MO2.

Phenotypes:
Xla Wt + erfe MO + WE NF35/36 (RNA-Seq (RNA))

Article: XB-ART-56559, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
WE - NF35/36 GSM3589901  GSM3589916  GSM3589931  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 35 and 36  
   WE + erfe MO - NF35/36 DEG GSM3589903  GSM3589918  GSM3589933  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO1 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 35 and 36  
   WE + erfe MO - NF35/36 DEG GSM3589905  GSM3589920  GSM3589935  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO2 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 35 and 36  
   WE + hbg1 cMO - NF35/36 DEG GSM3589902  GSM3589917  GSM3589932  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 35 and 36  
   WE + hbg1 cMO - NF35/36 DEG GSM3589904  GSM3589919  GSM3589934  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 35 and 36  
WE - NF37/38 GSM3589906  GSM3589921  GSM3589936  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 37 and 38  
   WE + erfe MO - NF37/38 DEG GSM3589908  GSM3589923  GSM3589938  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO1 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 37 and 38  
   WE + erfe MO - NF37/38 DEG GSM3589910  GSM3589925  GSM3589940  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO2 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 37 and 38  
   WE + hbg1 cMO - NF37/38 DEG GSM3589907  GSM3589922  GSM3589937  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 37 and 38  
   WE + hbg1 cMO - NF37/38 DEG GSM3589909  GSM3589924  GSM3589939  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 37 and 38  
WE - NF41 GSM3589911  GSM3589926  GSM3589941  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 41  
   WE + erfe MO - NF41 DEG GSM3589913  GSM3589928  GSM3589943  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO1 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 41  
   WE + erfe MO - NF41 DEG GSM3589915  GSM3589930  GSM3589945  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection erfe MO2 erfe (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 41  
   WE + hbg1 cMO - NF41 DEG GSM3589912  GSM3589927  GSM3589942  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 41  
   WE + hbg1 cMO - NF41 DEG GSM3589914  GSM3589929  GSM3589944  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection   hbg1 (Xla) NF stage 4 (8-cell) blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 41  
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