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Mol Cell Biol
1998 Mar 01;183:1467-76. doi: 10.1128/MCB.18.3.1467.
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Repression of TFIIH transcriptional activity and TFIIH-associated cdk7 kinase activity at mitosis.
Long JJ, Leresche A, Kriwacki RW, Gottesfeld JM.
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Nuclear transcription is repressed when eukaryotic cells enter mitosis. Mitotic repression of transcription of various cellular and viral gene promoters by RNA polymerase II can be reproduced in vitro either with extracts prepared from cells arrested at mitosis with the microtubule polymerization inhibitor nocodazole or with nuclear extracts prepared from asynchronous cells and the mitotic protein kinase cdc2/cyclin B. Purified cdc2/cyclin B kinase is also sufficient to inhibit transcription in reconstituted transcription reactions with biochemically purified and recombinant basal transcription factors and RNA polymerase II. The cyclin-dependent kinase inhibitor p21Waf1/Cip1/Sdi1 can reverse the effect of cdc2/cyclin B kinase, indicating that repression of transcription is due to protein phosphorylation. Transcription rescue and inhibition experiments with each of the basal factors and the polymerase suggest that multiple components of the transcription machinery are inactivated by cdc2/cyclin B kinase. For an activated promoter, targets of repression are TFIID and TFIIH, while for a basal promoter, TFIIH is the major target for mitotic inactivation of transcription. Protein labeling experiments indicate that the p62 and p36 subunits of TFIIH are in vitro substrates for mitotic phosphorylation. Using the carboxy-terminal domain of the large subunit of RNA polymerase II as a test substrate for phosphorylation, the TFIIH-associated kinase, cdk7/cyclin H, is inhibited concomitant with inhibition of transcription activity. Our results suggest that there exist multiple phosphorylation targets for the global shutdown of transcription at mitosis.
Adamczewski,
MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.
1996, Pubmed
Adamczewski,
MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.
1996,
Pubmed Buermeyer,
The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.
1992,
Pubmed Cisek,
Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2.
1989,
Pubmed Dahmus,
Phosphorylation of the C-terminal domain of RNA polymerase II.
1995,
Pubmed Dahmus,
The role of multisite phosphorylation in the regulation of RNA polymerase II activity.
1994,
Pubmed Dignam,
Eukaryotic gene transcription with purified components.
1983,
Pubmed
,
Xenbase Drapkin,
The multifunctional TFIIH complex and transcriptional control.
1994,
Pubmed el-Deiry,
WAF1, a potential mediator of p53 tumor suppression.
1993,
Pubmed Fischer,
Cloning of the 62-kilodalton component of basic transcription factor BTF2.
1992,
Pubmed Flores,
Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.
1992,
Pubmed Gebara,
Phosphorylation of the carboxy-terminal repeat domain in RNA polymerase II by cyclin-dependent kinases is sufficient to inhibit transcription.
1997,
Pubmed Gottesfeld,
Mitotic repression of the transcriptional machinery.
1997,
Pubmed Gottesfeld,
Mitotic repression of RNA polymerase III transcription in vitro mediated by phosphorylation of a TFIIIB component.
1994,
Pubmed
,
Xenbase Harper,
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
1993,
Pubmed Harper,
Inhibition of cyclin-dependent kinases by p21.
1995,
Pubmed Hartl,
Mitotic repression of transcription in vitro.
1993,
Pubmed
,
Xenbase Hernandez,
TBP, a universal eukaryotic transcription factor?
1993,
Pubmed Hershkovitz,
Metaphase chromosome analysis by ligation-mediated PCR: heritable chromatin structure and a comparison of active and inactive X chromosomes.
1995,
Pubmed Hsu,
Cell cycle regulation of glucocorticoid receptor function.
1992,
Pubmed Hunter,
The regulation of transcription by phosphorylation.
1992,
Pubmed Jackson,
Regulating transcription factor activity by phosphorylation.
1992,
Pubmed Johnson,
Ribonucleic acid and protein synthesis in mitotic HeLa cells.
1965,
Pubmed Kim,
The major late promoter of adenovirus-2 is accurately transcribed by RNA polymerases IIO, IIA, and IIB.
1989,
Pubmed Kim,
Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA.
1997,
Pubmed Kriwacki,
Structural studies of p21Waf1/Cip1/Sdi1 in the free and Cdk2-bound state: conformational disorder mediates binding diversity.
1996,
Pubmed Lee,
Inhibition of the association of RNA polymerase II with the preinitiation complex by a viral transcriptional repressor.
1996,
Pubmed Leresche,
Repression of RNA polymerase II and III transcription during M phase of the cell cycle.
1996,
Pubmed
,
Xenbase Li,
Interplay of positive and negative effectors in function of the C-terminal repeat domain of RNA polymerase II.
1994,
Pubmed Lu,
The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex.
1991,
Pubmed Lüscher,
Mitosis-specific phosphorylation of the nuclear oncoproteins Myc and Myb.
1992,
Pubmed Mäkelä,
A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription.
1995,
Pubmed Martínez-Balbás,
Displacement of sequence-specific transcription factors from mitotic chromatin.
1995,
Pubmed Orphanides,
The general transcription factors of RNA polymerase II.
1996,
Pubmed Parvin,
Promoter specificity of basal transcription factors.
1992,
Pubmed Peterson,
DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats.
1992,
Pubmed PRESCOTT,
Synthesis of RNA and protein during mitosis in mammalian tissue culture cells.
1962,
Pubmed Proudfoot,
Structure and in vitro transcription of human globin genes.
1980,
Pubmed Sawadogo,
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
1985,
Pubmed Schaeffer,
DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor.
1993,
Pubmed Segil,
Mitotic regulation of TFIID: inhibition of activator-dependent transcription and changes in subcellular localization.
1996,
Pubmed Serizawa,
A carboxyl-terminal-domain kinase associated with RNA polymerase II transcription factor delta from rat liver.
1992,
Pubmed Sheridan,
Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro.
1995,
Pubmed Shiekhattar,
Cdk-activating kinase complex is a component of human transcription factor TFIIH.
1995,
Pubmed Smale,
The "initiator" as a transcription control element.
1989,
Pubmed Smythe,
Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2.
1992,
Pubmed
,
Xenbase Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase Svejstrup,
The multiple roles of transcription/repair factor TFIIH.
1996,
Pubmed Usheva,
Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein.
1992,
Pubmed White,
Mitotic regulation of a TATA-binding-protein-containing complex.
1995,
Pubmed Wolf,
Role of maturation-promoting factor (p34cdc2-cyclin B) in differential expression of the Xenopus oocyte and somatic-type 5S RNA genes.
1994,
Pubmed
,
Xenbase Xiong,
p21 is a universal inhibitor of cyclin kinases.
1993,
Pubmed Yankulov,
Regulation of CDK7 substrate specificity by MAT1 and TFIIH.
1997,
Pubmed Zawel,
The cycling of RNA polymerase II during transcription.
1993,
Pubmed Zehring,
The C-terminal repeat domain of RNA polymerase II largest subunit is essential in vivo but is not required for accurate transcription initiation in vitro.
1988,
Pubmed Zhang,
p21 is a component of active cell cycle kinases.
1994,
Pubmed Zhou,
Novel mechanism and factor for regulation by HIV-1 Tat.
1995,
Pubmed
