Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
1. The preparation of ribosomes and ribosomal subunits from Xenopus ovary is described. 2. The yield of once-washed ribosomes (buoyant density in caesium chloride 1.601g.cm(-3); 44% RNA, 56% protein by chemical methods) was 10.1mg/g wet wt. of tissue. 3. Buoyant density in caesium chloride and RNA/protein ratios by chemical methods have been determined for ribosome subunits produced by 1.0mm-EDTA or 0.5m-potassium chloride treatment and also for EDTA subunits extracted with 0.5m-, 1.0m- or 1.5m-potassium chloride, 4. Analysis of ribosomal protein on acrylamide gels at pH4.5 in 6m-urea reveals 24 and 26 bands from small and large EDTA subunits respectively. The actual numbers of proteins are greater than this, as many bands are obviously doublets. 5. Analysis of the proteins in the potassium chloride extract and particle fractions showed that some bands are completely and some partially extracted. Taking partial extraction as an indication of possible doublet bands it was found that there were 12 and 20 such bands in the small and large subunits respectively, making totals of 36 and 46 proteins. 6. From the measured protein contents and assuming weight-average molecular weights for the proteins of large and small subunits close to those observed for eukaryote ribosomal proteins it is possible to compute the total numbers of protein molecules per particle. It appears that too few protein bands have been identified on acrylamide gels to account for all the protein in the large subunit, but probably enough for the small subunit.
Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968, Pubmed,
Xenbase
Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase
BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase
Clegg,
The controlled dissociation of proteins from rat liver ribosomes by potassium chloride.
1970,
Pubmed
Craven,
The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins.
1969,
Pubmed
Eikenberry,
Separation of large quantities of ribosomal subunits by zonal ultracentrifugation.
1970,
Pubmed
FLECK,
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.
1962,
Pubmed
Gould,
Proteins of rabbit reticulocyte ribosomal subunits.
1970,
Pubmed
Grummt,
Stepwise dissociation of rat-liver ribosomes into core particles and split proteins.
1970,
Pubmed
Hamilton,
The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits.
1969,
Pubmed
Hamilton,
Characterization of some of the proteins of the large subunit of rat liver ribosomes.
1967,
Pubmed
Hardy,
The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
1969,
Pubmed
Itoh,
Release of ribosomal proteins from Escherichia coli ribosomes with high concentrations of lithium chloride.
1968,
Pubmed
KATZ,
A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID.
1963,
Pubmed
Kurland,
Ribosome structure and function emergent.
1970,
Pubmed
LEBOY,
THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.
1964,
Pubmed
Loening,
Properties of the ribosomal RNA precursor in Xenopus laevis; comparison to the precursor in mammals and in plants.
1969,
Pubmed
,
Xenbase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Martin,
Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomes.
1969,
Pubmed
MESELSON,
CONSERVATION OF RIBOSOMES DURING BACTERIAL GROWTH.
1964,
Pubmed
Moore,
Ribosomal proteins of Escherichia coli. II. Proteins from the 30 s subunit.
1968,
Pubmed
Nomura,
Bacterial ribosome.
1970,
Pubmed
Reboud,
The partial extraction of proteins from rat liver ribosomes, and the physical properties of the residual nucleoprotein particles.
1969,
Pubmed
Spitnik-Elson,
Detachment of ribosomal proteins by salt. I. Effect of conditions on the amount of protein detached.
1969,
Pubmed
