Methods Mol Biol 2015 Jan 01;1266:93-106. doi: 10.1007/978-1-4939-2272-7_6.

Fluorescent labeling for patch-clamp fluorometry (PCF) measurements of real-time protein motion in ion channels.

???displayArticle.abstract???
Understanding the function of ion channels is a major goal of molecular neurophysiology. While standard electrophysiological methods are invaluable tools to investigate the gating of ion channels, the structural rearrangements that mediate the way a channel senses physiological signals and opens and closes its gates cannot be measured electrically in a direct way. Here, we describe a method, based on site-specific labeling of a channel of interest with an environmentally sensitive fluorophore, which makes it possible to monitor conformational changes of ion channels in biological membranes in real time.

???displayArticle.pubmedLink??? 25560069
???displayArticle.link??? Methods Mol Biol
???displayArticle.grants??? [+]