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Invest Ophthalmol Vis Sci
2005 Dec 01;4612:4762-71. doi: 10.1167/iovs.05-0797.
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Cone survival despite rod degeneration in XOPS-mCFP transgenic zebrafish.
Morris AC
,
Schroeter EH
,
Bilotta J
,
Wong RO
,
Fadool JM
.
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In animal models of retinitis pigmentosa, rod photoreceptor degeneration eventually leads to loss of cone photoreceptors. The purpose of this study was to characterize a transgenic model of rod degeneration in zebrafish. Zebrafish transgenic for XOPS-mCFP, a membrane-targeted form of cyan fluorescent protein driven by the Xenopus rhodopsin promoter, were generated by plasmid injection. Immunohistochemistry was used to detect cell type, proliferation, and TUNEL markers in larval and adult retinas. Rod- and cone-specific transcripts were detected by RT-PCR. Visual responses in transgenic adults were measured by electroretinogram. The XOPS promoter directed specific expression of mCFP in rods by 55 hours post fertilization (hpf). Rods in XOPS-mCFP heterozygotes began dying at 3.5 days post fertilization (dpf) and were almost completely absent by 5 dpf. A few rods were observed at the retinal margin, and numerous immature rods were observed in the outer nuclear layer (ONL) of transgenic adults. Apoptosis was increased in the ONL of larval and adult transgenic animals, and an elevation of rod precursor proliferation in adults was observed. ERG analysis confirmed that rod responses were absent in this line. Cone morphology and electrophysiology appeared normal in transgenic animals up to 7 months of age. The XOPS-mCFP transgene causes selective degeneration of rods without secondary loss of cones in animals up to 7 months of age. This raises important questions about the significance of rod-cone interactions in zebrafish and their potential as a model of human inherited retinal degenerations.
Alfinito,
Activation of mislocalized opsin kills rod cells: a novel mechanism for rod cell death in retinal disease.
2002, Pubmed
Alfinito,
Activation of mislocalized opsin kills rod cells: a novel mechanism for rod cell death in retinal disease.
2002,
Pubmed
Batni,
Characterization of the Xenopus rhodopsin gene.
1996,
Pubmed
,
Xenbase
Biehlmaier,
Onset and time course of apoptosis in the developing zebrafish retina.
2001,
Pubmed
Braisted,
Selective regeneration of photoreceptors in goldfish retina.
1994,
Pubmed
Brockerhoff,
A behavioral screen for isolating zebrafish mutants with visual system defects.
1995,
Pubmed
Cameron,
Cellular proliferation and neurogenesis in the injured retina of adult zebrafish.
2000,
Pubmed
Chan,
Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy.
2004,
Pubmed
Chinen,
Gene duplication and spectral diversification of cone visual pigments of zebrafish.
2003,
Pubmed
Cideciyan,
Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man.
1998,
Pubmed
DeCarvalho,
Developmental expression of the POU domain transcription factor Brn-3b (Pou4f2) in the lateral line and visual system of zebrafish.
2004,
Pubmed
Dryja,
A point mutation of the rhodopsin gene in one form of retinitis pigmentosa.
1990,
Pubmed
Egensperger,
Fate of DNA from retinal cells dying during development: uptake by microglia and macroglia (Müller cells).
1996,
Pubmed
Fadool,
Development of a rod photoreceptor mosaic revealed in transgenic zebrafish.
2003,
Pubmed
,
Xenbase
Fadool,
Mutations affecting eye morphology in the developing zebrafish (Danio rerio).
1997,
Pubmed
Goldsmith,
Two zebrafish mutants, ebony and ivory, uncover benefits of neighborhood on photoreceptor survival.
2003,
Pubmed
Hagedorn,
Retinal growth and cell addition during embryogenesis in the teleost, Haplochromis burtoni.
1992,
Pubmed
Hamaoka,
Visualization of rod photoreceptor development using GFP-transgenic zebrafish.
2002,
Pubmed
Hughes,
Cone contributions to the photopic spectral sensitivity of the zebrafish ERG.
1998,
Pubmed
Hyatt,
Retinoic acid alters photoreceptor development in vivo.
1996,
Pubmed
Ichsan,
Rhodopsin promoter-EGFP fusion transgene expression in photoreceptor neurons of retina and pineal complex in mice.
2005,
Pubmed
Jin,
An improved rhodopsin/EGFP fusion protein for use in the generation of transgenic Xenopus laevis.
2003,
Pubmed
,
Xenbase
Johns,
Genesis of rods in teleost fish retina.
,
Pubmed
Kay,
Transient requirement for ganglion cells during assembly of retinal synaptic layers.
2004,
Pubmed
Kay,
Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina.
2005,
Pubmed
Kedzierski,
Non-cell-autonomous photoreceptor degeneration in rds mutant mice mosaic for expression of a rescue transgene.
1998,
Pubmed
Kennedy,
Isolation of a zebrafish rod opsin promoter to generate a transgenic zebrafish line expressing enhanced green fluorescent protein in rod photoreceptors.
2001,
Pubmed
Li,
Transgenic mice carrying the dominant rhodopsin mutation P347S: evidence for defective vectorial transport of rhodopsin to the outer segments.
1996,
Pubmed
Li,
A dominant form of inherited retinal degeneration caused by a non-photoreceptor cell-specific mutation.
1997,
Pubmed
Li,
Rhodopsin transgenic pigs as a model for human retinitis pigmentosa.
1998,
Pubmed
Léveillard,
Identification and characterization of rod-derived cone viability factor.
2004,
Pubmed
Maaswinkel,
ENU-induced late-onset night blindness associated with rod photoreceptor cell degeneration in zebrafish.
2003,
Pubmed
Malicki,
Mutations affecting development of the zebrafish retina.
1996,
Pubmed
Menu dit Huart,
DNA repair in the degenerating mouse retina.
2004,
Pubmed
Moench,
Fluorescence labeling of the palmitoylation sites of rhodopsin.
1994,
Pubmed
Mohand-Said,
Rod-cone interactions: developmental and clinical significance.
2001,
Pubmed
Moritz,
A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern.
2001,
Pubmed
,
Xenbase
Moritz,
Mutant rab8 Impairs docking and fusion of rhodopsin-bearing post-Golgi membranes and causes cell death of transgenic Xenopus rods.
2001,
Pubmed
,
Xenbase
Neuhauss,
Genetic disorders of vision revealed by a behavioral screen of 400 essential loci in zebrafish.
1999,
Pubmed
Nour,
Absence of functional and structural abnormalities associated with expression of EGFP in the retina.
2004,
Pubmed
Nour,
Mouse models of human retinal disease caused by expression of mutant rhodopsin. A valuable tool for the assessment of novel gene therapies.
2003,
Pubmed
Olsson,
Transgenic mice with a rhodopsin mutation (Pro23His): a mouse model of autosomal dominant retinitis pigmentosa.
1992,
Pubmed
Perkins,
Transgenic expression of a GFP-rhodopsin COOH-terminal fusion protein in zebrafish rod photoreceptors.
2002,
Pubmed
Peterson,
Müller cell differentiation in the zebrafish neural retina: evidence of distinct early and late stages in cell maturation.
2001,
Pubmed
Raymond,
Germinal cells in the goldfish retina that produce rod photoreceptors.
1987,
Pubmed
Raymond,
Regeneration of goldfish retina: rod precursors are a likely source of regenerated cells.
1988,
Pubmed
Raymond,
Retinal regeneration: common principles but a diversity of mechanisms.
1997,
Pubmed
Ripps,
Cell death in retinitis pigmentosa: gap junctions and the 'bystander' effect.
2002,
Pubmed
Rivolta,
Retinitis pigmentosa and allied diseases: numerous diseases, genes, and inheritance patterns.
2002,
Pubmed
Saszik,
The effects of temperature on the dark-adapted spectral sensitivity function of the adult zebrafish.
1999,
Pubmed
Sung,
A rhodopsin gene mutation responsible for autosomal dominant retinitis pigmentosa results in a protein that is defective in localization to the photoreceptor outer segment.
1994,
Pubmed
Tsujikawa,
Intraflagellar transport genes are essential for differentiation and survival of vertebrate sensory neurons.
2004,
Pubmed
Vihtelic,
Light-induced rod and cone cell death and regeneration in the adult albino zebrafish (Danio rerio) retina.
2000,
Pubmed
Vihtelic,
Cloning and characterization of six zebrafish photoreceptor opsin cDNAs and immunolocalization of their corresponding proteins.
1999,
Pubmed
Wang,
Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin.
2005,
Pubmed
