Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-10847
Nat Cell Biol June 1, 2000; 2 (6): 358-64.
Show Gene links Show Anatomy links

A new function for the gamma-tubulin ring complex as a microtubule minus-end cap.

Wiese C , Zheng Y .


Abstract
Microtubule nucleation from centrosomes involves a lockwasher-shaped protein complex containing gamma-tubulin, named the gamma-tubulin ring complex (gammaTuRC). Here we investigate the mechanism by which the gammaTuRC nucleates microtubules, using a direct labelling method to visualize the behaviour of individual gammaTuRCs. A fluorescently-labelled version of the gammaTuRC binds to the minus ends of microtubules nucleated in vitro. Both gammaTuRC-mediated nucleation and binding of the gammaTuRC to preformed microtubules block further minus-end growth and prevent microtubule depolymerization. The gammaTuRC therefore acts as a minus-end-capping protein, as confirmed by electron-microscopic examination of gold-labelled gammaTuRCs. These data support a nucleation model for gammaTuRC function that involves capping of microtubules.

PubMed ID: 10854327
Article link: Nat Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: tubg1

References :
Erickson, Gamma-tubulin nucleation: template or protofilament? 2000, Pubmed