XB-ART-10848Nat Cell Biol June 1, 2000; 2 (6): 352-7.
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Immunostructural evidence for the template mechanism of microtubule nucleation.
Two opposing models have been proposed to explain how the gamma-tubulin ring complex (gammaTuRC) induces microtubule nucleation. In the ''protofilament'' model, the gammaTuRC induces nucleation as a partially or completely straightened protofilament that is incorporated longitudinally into the wall of the nascent microtubule, whereas the ''template'' model proposes that the gammaTuRC acts as a helical template that constitutes the base of the newly-formed polymer. Here we appraise these two models, using high-resolution structural and immunolocalization methods. We show that components of the gammaTuRC localize to a narrow zone at the extreme minus end of the microtubule and that these ends terminate in a pointed cap. Together, these results strongly favour the template model of microtubule nucleation.
PubMed ID: 10854326
Article link: Nat Cell Biol
Species referenced: Xenopus laevis
Genes referenced: tubg1
Erickson, Gamma-tubulin nucleation: template or protofilament? 2000, Pubmed