XB-ART-10889Mech Dev June 1, 2000; 94 (1-2): 243-5.
Xenopus frizzled 4 is a maternal mRNA and its zygotic expression is localized to the neuroectoderm and trunk lateral plate mesoderm.
We describe the identification and expression pattern of Xenopus frizzled 4 (Xfz4) gene during early development. Xfz4 protein presents characteristic features of a frizzled family member. The mature protein sequence of Xfz4 is 93% identical to murine Mfz4. Xfz4 is a maternal mRNA, its expression level remains constant during early development. The mRNA is first localized during gastrulation to the dorsal presumptive neuroectoderm. At the end of gastrulation, Xfz4 mRNA is detected in the dorso-anterior neuroectoderm. During neurulation, Xfz4 mRNA is expressed as a band on both side of the forebrain, and in the trunk lateral plate mesoderm. As development proceeds, expression of Xfz4 mRNA in the trunk lateral plate mesoderm decreases but persists in the forebrain. It is also expressed in the posterior unsegmented somitic mesoderm from late tail-bud stage onward.
PubMed ID: 10842080
Article link: Mech Dev
Species referenced: Xenopus
Genes referenced: fzd4
Article Images: [+] show captions
|Fig. 3. Whole-mount in situ hybridization analysis of Xfz4 mRNA expression. (A–C) Dorso-vegetal view. (A) Stage 10.5, Xfz4 mRNA is enriched in the dorsal ectoderm. (B) Stage 11, Expression of Xfz4 mRNA is further restricted to the neuroectoderm. Bl, dorsal blastopore. (C) Stage 12, Xfz4 mRNA is detected in the anterior neuroectoderm. yp, yolk plug. (D,E) Sagittal sections made from (B,C), respectively, showing expression of Xfz4 mRNA in the deep layer of neuroectoderm (arrows). (F) Stage 18. Anterior view shows the expression of Xfz4 mRNA as a band on both side of the forebrain. (G) Stage 18. Anterior-ventral view shows expression of Xfz4 mRNA in the flank region. Notice that the ventral region expresses low level of Xfz4 mRNA. (H) Stage 18. Dorsal view. Expression of Xfz4 mRNA in the flank narrows dorsally. (I) Stage 14 parasagittal section showing expression of Xfz4 mRNA in the thickened anterior neural plate (Arrows). (J) Stage 18 transverse section showing expression of Xfz4 mRNA in the lateral plate mesoderm (arrows). Notice that somites (S) and notochord do not express Xfz4 mRNA. (K) Stage 25. Ventral view. Strong expression of Xfz4 mRNA in the lateral plate mesoderm flanking the heart primordium. (L) Stage 28. Lateral view. Xfz4 mRNA is detected in the forebrain and posterior unsegmented somitic mesoderm.|