Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2005 Dec 01;2523:10528-32. doi: 10.1128/MCB.25.23.10528-10532.2005.
Show Gene links
Show Anatomy links
PSF1 is essential for early embryogenesis in mice.
Ueno M
,
Itoh M
,
Kong L
,
Sugihara K
,
Asano M
,
Takakura N
.
???displayArticle.abstract??? Psf1 (partner of sld five 1) forms a novel heterotetramer complex, GINS (Go, Ichi, Nii, and San; five, one, two, and three, respectively, in Japanese), with Sld5, Psf2, and Psf3. The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus laevis egg extracts. Although all of the components are well conserved in higher eukaryotes, the biological function in vivo is largely unknown. We originally cloned the mouse ortholog of PSF1 from a hematopoietic stem cell cDNA library and found that PSF1 is expressed in blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Here we used the gene-targeting technique to determine the physiological function of PSF1 in vivo. Mice homozygous for a nonfunctional mutant of PSF1 died in utero around the time of implantation. PSF1-/- blastocysts failed to show outgrowth in culture and exhibited a cell proliferation defect. Our data clearly indicate that PSF1 is required for early embryogenesis.
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997, Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Christensen,
Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation.
2003,
Pubmed
Diffley,
Two steps in the assembly of complexes at yeast replication origins in vivo.
1994,
Pubmed
,
Xenbase
Fisher,
Vertebrate HoxB gene expression requires DNA replication.
2003,
Pubmed
,
Xenbase
Forlani,
Relief of a repressed gene expression state in the mouse 1-cell embryo requires DNA replication.
1998,
Pubmed
Hopwood,
Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication.
1996,
Pubmed
Kanemaki,
Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo.
2003,
Pubmed
Kelly,
Regulation of chromosome replication.
2000,
Pubmed
Kubota,
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
2003,
Pubmed
,
Xenbase
Mimura,
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.
1998,
Pubmed
,
Xenbase
Nagy,
Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.
1993,
Pubmed
Prasanth,
Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance.
2004,
Pubmed
Raghuraman,
Replication dynamics of the yeast genome.
2001,
Pubmed
Schultz,
Regulation of zygotic gene activation in the mouse.
1993,
Pubmed
Schübeler,
Genome-wide DNA replication profile for Drosophila melanogaster: a link between transcription and replication timing.
2002,
Pubmed
Takakura,
Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis.
1998,
Pubmed
Takakura,
A role for hematopoietic stem cells in promoting angiogenesis.
2000,
Pubmed
Takayama,
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.
2003,
Pubmed
Tercero,
DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.
2000,
Pubmed
Weinreich,
The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin.
1999,
Pubmed
Winzeler,
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
1999,
Pubmed
Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase
Yoshida,
Requirement of CDC45 for postimplantation mouse development.
2001,
Pubmed
,
Xenbase
