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Plant Cell
2005 Dec 01;1712:3409-21. doi: 10.1105/tpc.105.035774.
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NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii.
Mussgnug JH
,
Wobbe L
,
Elles I
,
Claus C
,
Hamilton M
,
Fink A
,
Kahmann U
,
Kapazoglou A
,
Mullineaux CW
,
Hippler M
,
Nickelsen J
,
Nixon PJ
,
Kruse O
.
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Photosynthetic organisms respond to changes in ambient light by modulating the size and composition of their light-harvesting complexes, which in the case of the green alga Chlamydomonas reinhardtii consists of >15 members of a large extended family of chlorophyll binding subunits. How their expression is coordinated is unclear. Here, we describe the analysis of an insertion mutant, state transitions mutant3 (stm3), which we show has increased levels of LHCBM subunits associated with the light-harvesting antenna of photosystem II. The mutated nuclear gene in stm3 encodes the RNA binding protein NAB1 (for putative nucleic acid binding protein). In vitro and in vivo RNA binding and protein expression studies have confirmed that NAB1 differentially binds to LHCBM mRNA in a subpolysomal high molecular weight RNA-protein complex. Binding of NAB1 stabilizes LHCBM mRNA at the preinitiation level via sequestration and thereby represses translation. The specificity and affinity of binding are determined by an RNA sequence motif similar to that used by the Xenopus laevis translation repressor FRGY2, which is conserved to varying degrees in the LHCBM gene family. We conclude from our results that NAB1 plays an important role in controlling the expression of the light-harvesting antenna of photosystem II at the posttranscriptional level. The similarity of NAB1 and FRGY2 of Xenopus implies the existence of similar RNA-masking systems in animals and plants.
Allen,
Resolution of 16 to 20 chlorophyll-protein complexes using a low ionic strength native green gel system.
1991, Pubmed
Allen,
Resolution of 16 to 20 chlorophyll-protein complexes using a low ionic strength native green gel system.
1991,
Pubmed
Allen,
Protein phosphorylation in regulation of photosynthesis.
1992,
Pubmed
Allen,
Molecular recognition in thylakoid structure and function.
2001,
Pubmed
Anderson,
Functional dissection of circadian clock- and phytochrome-regulated transcription of the Arabidopsis CAB2 gene.
1995,
Pubmed
Barkan,
Proteins encoded by a complex chloroplast transcription unit are each translated from both monocistronic and polycistronic mRNAs.
1988,
Pubmed
Bonaventura,
Fluorescence and oxygen evolution from Chlorella pyrenoidosa.
1969,
Pubmed
Danon,
Translational regulation in the chloroplast.
1997,
Pubmed
Danon,
Light-regulated translation of chloroplast messenger RNAs through redox potential.
1994,
Pubmed
Depège,
Role of chloroplast protein kinase Stt7 in LHCII phosphorylation and state transition in Chlamydomonas.
2003,
Pubmed
Drapier,
A dominant nuclear mutation in Chlamydomonas identifies a factor controlling chloroplast mRNA stability by acting on the coding region of the atpA transcript.
2002,
Pubmed
Elrad,
A genome's-eye view of the light-harvesting polypeptides of Chlamydomonas reinhardtii.
2004,
Pubmed
Elrad,
A major light-harvesting polypeptide of photosystem II functions in thermal dissipation.
2002,
Pubmed
Engels,
Isolation, partial characterization and localization of a dihydrolipoamide dehydrogenase from the cyanobacterium Synechocystis PCC 6803.
1997,
Pubmed
Escoubas,
Light intensity regulation of cab gene transcription is signaled by the redox state of the plastoquinone pool.
1995,
Pubmed
Evdokimova,
The major mRNA-associated protein YB-1 is a potent 5' cap-dependent mRNA stabilizer.
2001,
Pubmed
Finazzi,
State transitions, cyclic and linear electron transport and photophosphorylation in Chlamydomonas reinhardtii.
1999,
Pubmed
Finazzi,
Photoinhibition of Chlamydomonas reinhardtii in State 1 and State 2: damages to the photosynthetic apparatus under linear and cyclic electron flow.
2001,
Pubmed
Fischer,
The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii.
2001,
Pubmed
Flachmann,
Accumulation of plant antenna complexes is regulated by post-transcriptional mechanisms in tobacco.
1995,
Pubmed
Fleischmann,
Isolation and characterization of photoautotrophic mutants of Chlamydomonas reinhardtii deficient in state transition.
1999,
Pubmed
Graumann,
A superfamily of proteins that contain the cold-shock domain.
1998,
Pubmed
,
Xenbase
Graumann,
A case of convergent evolution of nucleic acid binding modules.
1996,
Pubmed
Grossman,
Acclimation of Chlamydomonas reinhardtii to its nutrient environment.
2000,
Pubmed
Hillenkamp,
Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization.
1990,
Pubmed
Hippler,
Towards functional proteomics of membrane protein complexes: analysis of thylakoid membranes from Chlamydomonas reinhardtii.
2001,
Pubmed
Hoober,
The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. Elizabeth H. Harris. Academic Press, San Diego, CA, 1989. xiv, 780 pp., illus. $145.
1989,
Pubmed
Imbault,
Structure of the Chlamydomonas reinhardtii cabII-1 gene encoding a chlorophyll-a/b-binding protein.
1988,
Pubmed
Jones,
Induction of proteins in response to low temperature in Escherichia coli.
1987,
Pubmed
Kindle,
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
1990,
Pubmed
Lindahl,
Regulatory proteolysis of the major light-harvesting chlorophyll a/b protein of photosystem II by a light-induced membrane-associated enzymic system.
1995,
Pubmed
Manival,
RNA-binding strategies common to cold-shock domain- and RNA recognition motif-containing proteins.
2001,
Pubmed
,
Xenbase
Marco,
Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii.
1980,
Pubmed
Matsumoto,
Visualization of the reconstituted FRGY2-mRNA complexes by electron microscopy.
2003,
Pubmed
,
Xenbase
Matsumoto,
Translational repression dependent on the interaction of the Xenopus Y-box protein FRGY2 with mRNA. Role of the cold shock domain, tail domain, and selective RNA sequence recognition.
1996,
Pubmed
,
Xenbase
Maxwell,
Chlorophyll fluorescence--a practical guide.
2000,
Pubmed
Maxwell,
Redox Regulation of Light-Harvesting Complex II and cab mRNA Abundance in Dunaliella salina.
1995,
Pubmed
Millar,
The regulation of circadian period by phototransduction pathways in Arabidopsis.
1995,
Pubmed
Moore,
From birth to death: the complex lives of eukaryotic mRNAs.
2005,
Pubmed
Murata,
Control of excitation transfer in photosynthesis. I. Light-induced change of chlorophyll a fluorescence in Porphyridium cruentum.
1969,
Pubmed
Nelson,
The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.
1994,
Pubmed
Ossenbühl,
cis- and trans-Acting determinants for translation of psbD mRNA in Chlamydomonas reinhardtii.
2000,
Pubmed
Petracek,
A Chlamydomonas protein that binds single-stranded G-strand telomere DNA.
1994,
Pubmed
Petracek,
Light-regulated changes in abundance and polyribosome association of ferredoxin mRNA are dependent on photosynthesis.
1997,
Pubmed
Schönfeld,
The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii.
2004,
Pubmed
Shama,
The translational cis-regulatory element of mammalian ribosomal protein mRNAs is recognized by the plant translational apparatus.
1996,
Pubmed
Shepherd,
Regulation of light-harvesting chlorophyll-binding protein (LHCP) mRNA accumulation during the cell cycle in Chlamydomonas reinhardi.
1983,
Pubmed
Stauber,
Proteomics of Chlamydomonas reinhardtii light-harvesting proteins.
2003,
Pubmed
Stevens,
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
1996,
Pubmed
Strauss,
Ligation-mediated suppression-PCR as a powerful tool to analyse nuclear gene sequences in the green alga Chlamydomonas reinhardtii.
2001,
Pubmed
Tang,
Light control of nuclear gene mRNA abundance and translation in tobacco.
2003,
Pubmed
Teramoto,
Light-intensity-dependent expression of Lhc gene family encoding light-harvesting chlorophyll-a/b proteins of photosystem II in Chlamydomonas reinhardtii.
2002,
Pubmed
Yang,
The redox state of the plastoquinone pool controls the level of the light-harvesting chlorophyll a/b binding protein complex II (LHC II) during photoacclimation.
2001,
Pubmed
Yohn,
Altered mRNA binding activity and decreased translational initiation in a nuclear mutant lacking translation of the chloroplast psbA mRNA.
1996,
Pubmed