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XB-ART-1118
Dev Dyn February 1, 2006; 235 (2): 368-81.

Genomic profiling of mixer and Sox17beta targets during Xenopus endoderm development.

Dickinson K , Leonard J , Baker JC .


Abstract
The transcription factors Mixer and Sox17beta have well-characterized roles in endoderm specification during Xenopus embryogenesis. In order to more thoroughly understand the mechanisms by which these endodermal regulators act, we expressed Mixer and Sox17beta in naïve ectodermal tissue and, using oligonucleotide-based microarrays, compared their genomic transcriptional profile to that of unaffected tissue. Using this approach, we identified 71 transcripts that are upregulated by Mixer or Sox17beta, 63 of which have previously uncharacterized roles in endoderm development. Furthermore, an in situ hybridization screen using antisense probes for several of these clones identified six targets of Mixer and/or Sox17beta that are expressed in the endoderm during gastrula stages, providing new and regional markers of the endoderm. Our results contribute further insight into the functions of Mixer and Sox17beta and bring us closer to understanding at the molecular level the pathways that regulate endoderm development.

PubMed ID: 16278889
PMC ID: PMC4510981
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ascl1 btbd6 btg4 cdc42ep4 clcnkb cldn5 cmtm8 cpeb1 cxcr4 dmrta2 elf2 elovl2 eomes fgfr2 foxa1 foxc1 foxd1 gata5 gpr4 gsc hbg1 hprt1 irf1 irx4 itln1 itln2 kcnk6 lrsam1 mab21l2 marchf8 mgc69520 mig30 mixer msx2 myc nr2f2 otx2 pcdh10 pdgfra pisd-like.2 plk2 psmg2 rasl11b retreg2 sgk1 sox17a sox17b.1 sox17b.2 sp8 tbxt trabd2b traf4 tubg1 twist1 txnl4b usp33 wnt11b XB22041735 [provisional:rnf220] XB5814120 [provisional] XB5882685 [provisional:cdca7]

GEO Series: GSE3334: NCBI

Article Images: [+] show captions
References [+] :
Afouda, GATA4, 5 and 6 mediate TGFbeta maintenance of endodermal gene expression in Xenopus embryos. 2005, Pubmed, Xenbase