Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Ca(2+)-permeable AMPA receptors and spontaneous presynaptic transmitter release at developing excitatory spinal synapses.
Rohrbough J
,
Spitzer NC
.
???displayArticle.abstract???
At many mature vertebrate glutamatergic synapses, excitatory transmission strength and plasticity are regulated by AMPA and NMDA receptor (AMPA-R and NMDA-R) activation and by patterns of presynaptic transmitter release. Both receptors potentially direct neuronal differentiation by mediating postsynaptic Ca(2+) influx during early development. However, the development of synaptic receptor expression and colocalization has been examined developmentally in only a few systems, and changes in release properties at neuronal synapses have not been characterized extensively. We recorded miniature EPSCs (mEPSCs) from spinal interneurons in Xenopus embryos and larvae. In mature 5-8 d larvae, approximately 70% of mEPSCs in Mg(2+)-free saline are composed of both a fast AMPA-R-mediated component and a slower NMDA-R-mediated decay, indicating receptor colocalization at most synapses. By contrast, in 39-40 hr embryos approximately 65% of mEPSCs are exclusively fast, suggesting that these synapses initially express predominantly AMPA-R. In a physiological Mg(2+) concentration (1 mM), mEPSCs throughout development are mainly AMPA-R-mediated at negative potentials. Embryonic synaptic AMPA-R are highly Ca(2+)-permeable, mEPSC amplitude is over twofold larger than at mature synapses, and mEPSCs frequently occur in bursts consistent with asynchronous multiquantal release. AMPA-R function in this motor pathway thus appears to be independent of previous NMDA-R activation, unlike other regions of the developing nervous system, ensuring a greater reliability for embryonic excitatory transmission. Early spontaneous excitatory activity is specialized to promote AMPA-R-mediated synaptic Ca(2+) influx, which likely has significant roles in neuronal development.
Auger,
Multivesicular release at single functional synaptic sites in cerebellar stellate and basket cells.
1998, Pubmed
Auger,
Multivesicular release at single functional synaptic sites in cerebellar stellate and basket cells.
1998,
Pubmed
Bekkers,
Quantal analysis of EPSCs recorded from small numbers of synapses in hippocampal cultures.
1995,
Pubmed
Bekkers,
NMDA and non-NMDA receptors are co-localized at individual excitatory synapses in cultured rat hippocampus.
1989,
Pubmed
Bekkers,
Origin of variability in quantal size in cultured hippocampal neurons and hippocampal slices.
1990,
Pubmed
Ben-Ari,
GABAA, NMDA and AMPA receptors: a developmentally regulated 'ménage à trois'.
1997,
Pubmed
Benke,
Modulation of AMPA receptor unitary conductance by synaptic activity.
1998,
Pubmed
Bennett,
Probabilistic secretion of quanta: spontaneous release at active zones of varicosities, boutons, and endplates.
1995,
Pubmed
Borst,
Large amplitude variability of GABAergic IPSCs in melanotropes from Xenopus laevis: evidence that quantal size differs between synapses.
1994,
Pubmed
,
Xenbase
Carmignoto,
Activity-dependent decrease in NMDA receptor responses during development of the visual cortex.
1992,
Pubmed
Clarke,
Interneurones in the Xenopus embryo spinal cord: sensory excitation and activity during swimming.
1984,
Pubmed
,
Xenbase
Constantine-Paton,
LTP and activity-dependent synaptogenesis: the more alike they are, the more different they become.
1998,
Pubmed
Craig,
Activity and synaptic receptor targeting: the long view.
1998,
Pubmed
Crair,
A critical period for long-term potentiation at thalamocortical synapses.
1995,
Pubmed
Dale,
Experimentally derived model for the locomotor pattern generator in the Xenopus embryo.
1995,
Pubmed
,
Xenbase
Dale,
Dual-component amino-acid-mediated synaptic potentials: excitatory drive for swimming in Xenopus embryos.
1985,
Pubmed
,
Xenbase
Donevan,
GYKI 52466, a 2,3-benzodiazepine, is a highly selective, noncompetitive antagonist of AMPA/kainate receptor responses.
1993,
Pubmed
Durand,
Long-term potentiation and functional synapse induction in developing hippocampus.
1996,
Pubmed
Feldman,
Experience-dependent plasticity and the maturation of glutamatergic synapses.
1998,
Pubmed
Frerking,
Variation in GABA mini amplitude is the consequence of variation in transmitter concentration.
1995,
Pubmed
Frerking,
Are some minis multiquantal?
1997,
Pubmed
Gao,
Development of spontaneous synaptic transmission in the rat spinal cord.
1998,
Pubmed
Gilbertson,
Permeation of calcium ions through non-NMDA glutamate channels in retinal bipolar cells.
1991,
Pubmed
Gleason,
AMPA and NMDA receptors expressed by differentiating Xenopus spinal neurons.
1998,
Pubmed
,
Xenbase
Golshani,
Progression of change in NMDA, non-NMDA, and metabotropic glutamate receptor function at the developing corticothalamic synapse.
1998,
Pubmed
Gu,
Synaptic strengthening through activation of Ca2+-permeable AMPA receptors.
1996,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hestrin,
Developmental regulation of NMDA receptor-mediated synaptic currents at a central synapse.
1992,
Pubmed
Hori,
Miniature postsynaptic currents recorded from identified rat spinal dorsal horn projection neurons in thin-slice preparations.
1992,
Pubmed
Hsia,
Development of excitatory circuitry in the hippocampus.
1998,
Pubmed
Isaac,
Silent synapses during development of thalamocortical inputs.
1997,
Pubmed
Isaacson,
Receptors underlying excitatory synaptic transmission in slices of the rat anteroventral cochlear nucleus.
1995,
Pubmed
Isaacson,
Amplitude and time course of spontaneous and evoked excitatory postsynaptic currents in bushy cells of the anteroventral cochlear nucleus.
1996,
Pubmed
Jahr,
Calcium permeability of the N-methyl-D-aspartate receptor channel in hippocampal neurons in culture.
1993,
Pubmed
Jia,
Enhanced LTP in mice deficient in the AMPA receptor GluR2.
1996,
Pubmed
Johnson,
Glycine potentiates the NMDA response in cultured mouse brain neurons.
,
Pubmed
Jonas,
Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels.
1995,
Pubmed
Kullmann,
Extrasynaptic glutamate spillover in the hippocampus: evidence and implications.
1998,
Pubmed
Liao,
Deficiency in induction but not expression of LTP in hippocampal slices from young rats.
1996,
Pubmed
Liao,
Regulation of morphological postsynaptic silent synapses in developing hippocampal neurons.
1999,
Pubmed
Liao,
Activation of postsynaptically silent synapses during pairing-induced LTP in CA1 region of hippocampal slice.
1995,
Pubmed
Liu,
Properties of synaptic transmission at single hippocampal synaptic boutons.
1995,
Pubmed
Mahanty,
Calcium-permeable AMPA receptors mediate long-term potentiation in interneurons in the amygdala.
1998,
Pubmed
Malenka,
Silent synapses speak up.
1997,
Pubmed
Mayer,
Permeation and block of N-methyl-D-aspartic acid receptor channels by divalent cations in mouse cultured central neurones.
1987,
Pubmed
Neher,
Correction for liquid junction potentials in patch clamp experiments.
1992,
Pubmed
Nicoll,
Functional comparison of neurotransmitter receptor subtypes in mammalian central nervous system.
1990,
Pubmed
Nusser,
Differences in synaptic GABA(A) receptor number underlie variation in GABA mini amplitude.
1997,
Pubmed
O'Brien,
NMDA and non-NMDA receptors are co-localized at excitatory synapses of rat hypoglossal motoneurons.
1997,
Pubmed
O'Brien,
Activity-dependent modulation of synaptic AMPA receptor accumulation.
1998,
Pubmed
Otis,
AMPA receptors with high Ca2+ permeability mediate synaptic transmission in the avian auditory pathway.
1995,
Pubmed
Paternain,
Selective antagonism of AMPA receptors unmasks kainate receptor-mediated responses in hippocampal neurons.
1995,
Pubmed
Petralia,
Selective acquisition of AMPA receptors over postnatal development suggests a molecular basis for silent synapses.
1999,
Pubmed
Roberts,
The neuroanatomy of an amphibian embryo spinal cord.
1982,
Pubmed
,
Xenbase
Roberts,
Development and characterization of commissural interneurones in the spinal cord of Xenopus laevis embryos revealed by antibodies to glycine.
1988,
Pubmed
,
Xenbase
Rohrbough,
Regulation of intracellular Cl- levels by Na(+)-dependent Cl- cotransport distinguishes depolarizing from hyperpolarizing GABAA receptor-mediated responses in spinal neurons.
1996,
Pubmed
,
Xenbase
Scheetz,
Modulation of NMDA receptor function: implications for vertebrate neural development.
1994,
Pubmed
Scheetz,
N-methyl-D-aspartate receptor activation and visual activity induce elongation factor-2 phosphorylation in amphibian tecta: a role for N-methyl-D-aspartate receptors in controlling protein synthesis.
1997,
Pubmed
Sillar,
Presynaptic inhibition of primary afferent transmitter release by 5-hydroxytryptamine at a mechanosensory synapse in the vertebrate spinal cord.
1994,
Pubmed
,
Xenbase
Sillar,
Unmyelinated cutaneous afferent neurons activate two types of excitatory amino acid receptor in the spinal cord of Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Sillar,
Segregation of NMDA and non-NMDA receptors at separate synaptic contacts: evidence from spontaneous EPSPs in Xenopus embryo spinal neurons.
1991,
Pubmed
,
Xenbase
Soffe,
Activity of commissural interneurons in spinal cord of Xenopus embryos.
1984,
Pubmed
,
Xenbase
Turrigiano,
Activity-dependent scaling of quantal amplitude in neocortical neurons.
1998,
Pubmed
Vincent,
Neighboring cerebellar Purkinje cells communicate via retrograde inhibition of common presynaptic interneurons.
1993,
Pubmed
Wall,
Development of the quantal properties of evoked and spontaneous synaptic currents at a brain synapse.
1998,
Pubmed
Walmsley,
Diversity of structure and function at mammalian central synapses.
1998,
Pubmed
Walmsley,
Interpretation of 'quantal' peaks in distributions of evoked synaptic transmission at central synapses.
1995,
Pubmed
Wu,
Maturation of a central glutamatergic synapse.
1996,
Pubmed
,
Xenbase
Zhang,
Kinetic properties of NMDA receptors in embryonic Xenopus spinal neurons.
1995,
Pubmed
,
Xenbase
Zheng,
Essential role of filopodia in chemotropic turning of nerve growth cone induced by a glutamate gradient.
1996,
Pubmed
,
Xenbase
Ziskind-Conhaim,
NMDA receptors mediate poly- and monosynaptic potentials in motoneurons of rat embryos.
1990,
Pubmed
Zorumski,
A benzodiazepine recognition site associated with the non-NMDA glutamate receptor.
1993,
Pubmed
van den Pol,
Embryonic hypothalamic expression of functional glutamate receptors.
1995,
Pubmed
