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Mol Cell Biol
1999 Oct 01;1910:6682-9. doi: 10.1128/MCB.19.10.6682.
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Regulated formation of extrachromosomal circular DNA molecules during development in Xenopus laevis.
Cohen S
,
Menut S
,
Méchali M
.
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Extrachromosomal circular DNA molecules of chromosomal origin have been detected in many organisms and are thought to reflect genomic plasticity in eukaryotic cells. Here we report a developmentally regulated formation of extrachromosomal circular DNA that occurs de novo in preblastula Xenopus embryos. This specific DNA population is not detected in the male or female germ cells and is dramatically reduced in later developmental stages and in adult tissues. The activity responsible for the de novo production of extrachromosomal circles is maternally inherited, is stored in the unfertilized egg, and requires genomic DNA as a template. The formation of circular molecules does not require genomic DNA replication but both processes can occur simultaneously in the early development. The production of extrachromosomal circular DNA does not proceed at random since multimers of the tandemly repeated sequence satellite 1 were over-represented in the circle population, while other sequences (such as ribosomal DNA and JCC31 repeated sequence) were not detected. This phenomenon reveals an unexpected plasticity of the embryonic genome which is restricted to the early developmental stage.
Ackerman,
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
1983, Pubmed,
Xenbase
Ackerman,
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Brack,
A complete immunoglobulin gene is created by somatic recombination.
1978,
Pubmed
Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed
Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed
Busby,
Fate of amplified nucleoli in Xenopus laevis embryos.
1982,
Pubmed
,
Xenbase
Carroll,
Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length.
1976,
Pubmed
,
Xenbase
Carroll,
Adjacent repeating units of Xenopus laevis 5S DNA can be heterogeneous in length.
1976,
Pubmed
,
Xenbase
Chambers,
Dissection of a replication origin of Xenopus DNA.
1982,
Pubmed
,
Xenbase
Cohen,
Induction of circles of heterogeneous sizes in carcinogen-treated cells: two-dimensional gel analysis of circular DNA molecules.
1996,
Pubmed
Cohen,
Small polydispersed circular DNA (spcDNA) in human cells: association with genomic instability.
1997,
Pubmed
Coué,
Chromatin binding, nuclear localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication.
1996,
Pubmed
,
Xenbase
Dunon-Bluteau,
DNA synthesis in a mitochondrial lysate of Xenopus laevis oocytes. H strand replication in vitro.
1987,
Pubmed
,
Xenbase
Fujimoto,
Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.
,
Pubmed
Gaubatz,
Extrachromosomal circular DNAs and genomic sequence plasticity in eukaryotic cells.
1990,
Pubmed
Goto,
Highly repetitive DNA sequences that are restricted to the germ line in the hagfish Eptatretus cirrhatus: a mosaic of eliminated elements.
1998,
Pubmed
Gurdon,
Nuclear transplantation in eggs and oocytes.
1986,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hensey,
A developmental timer that regulates apoptosis at the onset of gastrulation.
1997,
Pubmed
,
Xenbase
Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed
Hourcade,
The amplification of ribosomal RNA genes involves a rolling circle intermediate.
1973,
Pubmed
,
Xenbase
Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase
Hyrien,
Transition in specification of embryonic metazoan DNA replication origins.
1995,
Pubmed
,
Xenbase
Kiyama,
A group of repetitive human DNA families that is characterized by extrachromosomal oligomers and restriction-fragment length polymorphism.
1987,
Pubmed
Kiyama,
A repetitive DNA family (Sau3A family) in human chromosomes: extrachromosomal DNA and DNA polymorphism.
1986,
Pubmed
Kubota,
A germline restricted, highly repetitive DNA sequence in Paramyxine atami: an interspecifically conserved, but somatically eliminated, element.
1997,
Pubmed
Kunisada,
Appearance of extrachromosomal circular DNAs during in vivo and in vitro ageing of mammalian cells.
1985,
Pubmed
Lam,
Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat).
1983,
Pubmed
,
Xenbase
Lehman,
Homologous and illegitimate recombination in developing Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase
Lin,
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
1984,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lund,
Control of 4-8S RNA transcription at the midblastula transition in Xenopus laevis embryos.
1992,
Pubmed
,
Xenbase
Matsuoka,
Switch circular DNA formed in cytokine-treated mouse splenocytes: evidence for intramolecular DNA deletion in immunoglobulin class switching.
1990,
Pubmed
Meyerhof,
Satellite DNA from Xenopus laevis: comparative analysis of 745 and 1037 base pair Hind III tandem repeats.
1983,
Pubmed
,
Xenbase
Moritz,
Complexity of germline and somatic DNA in Ascaris.
,
Pubmed
Motejlek,
Increased amount and contour length distribution of small polydisperse circular DNA (spcDNA) in Fanconi anemia.
1993,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Müller,
Chromatin diminution in nematodes.
1996,
Pubmed
Okazaki,
T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.
1987,
Pubmed
Plasterk,
Genetic switches: mechanism and function.
1992,
Pubmed
Pont,
Some extrachromosomal circular DNAs from Drosophila embryos are homologous to tandemly repeated genes.
1987,
Pubmed
Pont-Kingdon,
Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.
1993,
Pubmed
,
Xenbase
Prioleau,
Competition between chromatin and transcription complex assembly regulates gene expression during early development.
1994,
Pubmed
,
Xenbase
Reeder,
rRNA synthesis in the nucleolus.
1990,
Pubmed
Rochaix,
Ribosomal RNA gene amplification by rolling circles.
1974,
Pubmed
,
Xenbase
Sible,
Zygotic transcription is required to block a maternal program of apoptosis in Xenopus embryos.
1997,
Pubmed
,
Xenbase
Sinclair,
Extrachromosomal rDNA circles--a cause of aging in yeast.
1997,
Pubmed
Stack,
Developmentally regulated activation of apoptosis early in Xenopus gastrulation results in cyclin A degradation during interphase of the cell cycle.
1997,
Pubmed
,
Xenbase
Stanfield,
Small circular DNA in Drosophila melanogaster.
1976,
Pubmed
Sunnerhagen,
Increase of extrachromosomal circular DNA in mouse 3T6 cells on perturbation of DNA synthesis: implications for gene amplification.
1989,
Pubmed
Tlsty,
Genomic instability and cancer.
1995,
Pubmed
Vogt,
Potential genetic functions of tandem repeated DNA sequence blocks in the human genome are based on a highly conserved "chromatin folding code".
1990,
Pubmed
Wahl,
The importance of circular DNA in mammalian gene amplification.
1989,
Pubmed
Wellauer,
Arrangement of length heterogeneity in repeating units of amplified and chromosomal ribosomal DNA from Xenopus laevis.
1976,
Pubmed
,
Xenbase
von Schwedler,
Circular DNA is a product of the immunoglobulin class switch rearrangement.
1990,
Pubmed
