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Proc Natl Acad Sci U S A October 18, 2005; 102 (42): 15128-33.
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The Ca2+-induced methyltransferase xPRMT1b controls neural fate in amphibian embryo.

Batut J , Vandel L , Leclerc C , Daguzan C , Moreau M , Néant I .

We have previously shown that an increase in intracellular Ca2+ is both necessary and sufficient to commit ectoderm to a neural fate in Xenopus embryos. However, the relationship between this Ca2+ increase and the expression of early neural genes has yet to be defined. Using a subtractive cDNA library between untreated and caffeine-treated animal caps, i.e., control ectoderm and ectoderm induced toward a neural fate by a release of Ca2+, we have isolated the arginine N-methyltransferase, xPRMT1b, a Ca2+-induced target gene, which plays a pivotal role in this process. First, we show in embryo and in animal cap that xPRMT1b expression is Ca2+-regulated. Second, overexpression of xPRMT1b induces the expression of early neural genes such as Zic3. Finally, in the whole embryo, antisense approach with morpholino oligonucleotide against xPRMT1b impairs neural development and in animal caps blocks the expression of neural markers induced by a release of internal Ca2+. Our results implicate an instructive role of an enzyme, an arginine methyltransferase protein, in the embryonic choice of determination between epidermal and neural fate. The results presented provide insights by which a Ca2+ increase induces neural fate.

PubMed ID: 16214893
PMC ID: PMC1257693
Article link: Proc Natl Acad Sci U S A

Species referenced: Xenopus laevis
Genes referenced: acss2.2 bmp4 en2 h4c4 myod1 ncam1 neurog1 nog odc1 prmt1 sox15 tbxt tubb2b zic3
Morpholinos: prmt1 MO1

Article Images: [+] show captions
References [+] :
Aoki, Methylation of Xenopus CIRP2 regulates its arginine- and glycine-rich region-mediated nucleocytoplasmic distribution. 2002, Pubmed, Xenbase