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Biophys J
2006 Jan 01;901:140-50. doi: 10.1529/biophysj.105.066373.
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Conductance of connexin hemichannels segregates with the first transmembrane segment.
Hu X
,
Ma M
,
Dahl G
.
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Gap junction channels are intercellular channels that mediate the gated transfer of molecules between adjacent cells. To identify the domain determining channel conductance, the first transmembrane segment (M1) was reciprocally exchanged between Cx46 and Cx32E(1)43. The resulting chimeras exhibited conductances similar to that of the respective M1 donor. Furthermore, a chimera with the carboxy-terminal half of M1 in Cx46 replaced by that of Cx32 exhibited a conductance similar to that of Cx32E(1)43, whereas the chimera with only the amino-terminal half of M1 replaced retained the unitary conductance of wild-type Cx46. Extending the M1 domain swapping to other connexins by replacing the carboxy-terminal half of M1 in Cx46 with that of Cx37 yielded a chimera channel with increased unitary conductance close to that of Cx37. Furthermore, a point mutant of Cx46, with leucine substituted by glycine in position 35, displayed a conductance much larger than that of the wild type. Thus, the M1 segment, especially the second half, contains important determinants of conductance of the connexin channel.
Akabas,
Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the alpha subunit.
1994, Pubmed,
Xenbase
Akabas,
Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the alpha subunit.
1994,
Pubmed
,
Xenbase
Banach,
The influence of surface charges on the conductance of the human connexin37 gap junction channel.
2000,
Pubmed
Baranova,
The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins.
2004,
Pubmed
Beahm,
Hemichannel and junctional properties of connexin 50.
2002,
Pubmed
,
Xenbase
Beblo,
Monovalent cation permeation through the connexin40 gap junction channel. Cs, Rb, K, Na, Li, TEA, TMA, TBA, and effects of anions Br, Cl, F, acetate, aspartate, glutamate, and NO3.
1997,
Pubmed
Brelidze,
A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectification.
2003,
Pubmed
,
Xenbase
Bruzzone,
Pannexins, a family of gap junction proteins expressed in brain.
2003,
Pubmed
,
Xenbase
Bukauskas,
Gap junction channel gating.
2004,
Pubmed
Dahl,
Mutagenesis to study channel structure.
2001,
Pubmed
,
Xenbase
DeVries,
Hemi-gap-junction channels in solitary horizontal cells of the catfish retina.
1992,
Pubmed
Ebihara,
Properties of a nonjunctional current expressed from a rat connexin46 cDNA in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Ebihara,
Distinct behavior of connexin56 and connexin46 gap junctional channels can be predicted from the behavior of their hemi-gap-junctional channels.
1995,
Pubmed
,
Xenbase
Evans,
Gap junctions: structure and function (Review).
2002,
Pubmed
Fishman,
Functional analysis of human cardiac gap junction channel mutants.
1991,
Pubmed
Fleishman,
A Calpha model for the transmembrane alpha helices of gap junction intercellular channels.
2004,
Pubmed
Goodenough,
Beyond the gap: functions of unpaired connexon channels.
2003,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Harris,
Emerging issues of connexin channels: biophysics fills the gap.
2001,
Pubmed
Hartmann,
Exchange of conduction pathways between two related K+ channels.
1991,
Pubmed
,
Xenbase
Hofer,
Visualization and functional blocking of gap junction hemichannels (connexons) with antibodies against external loop domains in astrocytes.
1998,
Pubmed
Hu,
Exchange of conductance and gating properties between gap junction hemichannels.
1999,
Pubmed
,
Xenbase
Ji,
The role of Pre-H2 domains of alpha- and delta-epithelial Na+ channels in ion permeation, conductance, and amiloride sensitivity.
2004,
Pubmed
,
Xenbase
Kondo,
Metabolic inhibition activates a non-selective current through connexin hemichannels in isolated ventricular myocytes.
2000,
Pubmed
Kronengold,
Single-channel SCAM identifies pore-lining residues in the first extracellular loop and first transmembrane domains of Cx46 hemichannels.
2003,
Pubmed
,
Xenbase
Lopatin,
The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines.
1995,
Pubmed
,
Xenbase
Manthey,
Intracellular domains of mouse connexin26 and -30 affect diffusional and electrical properties of gap junction channels.
2001,
Pubmed
Musa,
Voltage-dependent blockade of connexin40 gap junctions by spermine.
2003,
Pubmed
Musa,
Amino terminal glutamate residues confer spermine sensitivity and affect voltage gating and channel conductance of rat connexin40 gap junctions.
2004,
Pubmed
Panchin,
A ubiquitous family of putative gap junction molecules.
2000,
Pubmed
Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Pfahnl,
A chimeric connexin forming gap junction hemichannels.
1997,
Pubmed
,
Xenbase
Pfahnl,
Localization of a voltage gate in connexin46 gap junction hemichannels.
1998,
Pubmed
,
Xenbase
Plum,
Unique and shared functions of different connexins in mice.
2000,
Pubmed
Shyng,
Depletion of intracellular polyamines relieves inward rectification of potassium channels.
1996,
Pubmed
,
Xenbase
Skerrett,
Identification of amino acid residues lining the pore of a gap junction channel.
2002,
Pubmed
,
Xenbase
Söhl,
Gap junctions and the connexin protein family.
2004,
Pubmed
,
Xenbase
Trexler,
Voltage gating and permeation in a gap junction hemichannel.
1996,
Pubmed
,
Xenbase
Trexler,
Rapid and direct effects of pH on connexins revealed by the connexin46 hemichannel preparation.
1999,
Pubmed
,
Xenbase
Trexler,
The first extracellular loop domain is a major determinant of charge selectivity in connexin46 channels.
2000,
Pubmed
,
Xenbase
Veenstra,
Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities.
1994,
Pubmed
Verselis,
Connexin hemichannels and cell-cell channels: comparison of properties.
2000,
Pubmed
,
Xenbase
Villmann,
Investigation by ion channel domain transplantation of rat glutamate receptor subunits, orphan receptors and a putative NMDA receptor subunit.
1999,
Pubmed
,
Xenbase
Williams,
Modulation and block of ion channels: a new biology of polyamines.
1997,
Pubmed
Williams,
Interactions of polyamines with ion channels.
1997,
Pubmed
Zhou,
Identification of a pore lining segment in gap junction hemichannels.
1997,
Pubmed
,
Xenbase
