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Development October 1, 2005; 132 (20): 4575-86.

The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo.

In this paper, we investigate the function of Smicl, a zinc-finger Smad-interacting protein that is expressed maternally in the Xenopus embryo. Inhibition of Smicl function by means of antisense morpholino oligonucleotides causes the specific downregulation of Chordin, a dorsally expressed gene encoding a secreted BMP inhibitor that is involved in mesodermal patterning and neural induction. Chordin is activated by Nodal-related signalling in an indirect manner, and we show here that Smicl is involved in a two-step process that is necessary for this activation. In the first, Smad3 (but not Smad2) activates expression of Xlim1 in a direct fashion. In the second, a complex containing Smicl and the newly induced Xlim1 induces expression of Chordin. As well as revealing the function of Smicl in the early embryo, our work yields important new insight in the regulation of Chordin and identifies functional differences between the activities of Smad2 and Smad3 in the Xenopus embryo.

PubMed ID: 16192311
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: chrd.1 gsc lhx1 mcf2 myc nodal nodal3.1 nodal3.2 odc1 smad1 smad2 smad3 smad4 smad4.2 sox17a tbxt zc3h3
Morpholinos: zc3h3 MO1 zc3h3 MO2 zc3h3 MO3

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