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The RNA-binding protein gene, hermes, is expressed at high levels in the developing heart.
Gerber WV
,
Yatskievych TA
,
Antin PB
,
Correia KM
,
Conlon RA
,
Krieg PA
.
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In a screen for novel sequences expressed during embryonic heart development we have isolated a gene which encodes a putative RNA-binding protein. This protein is a member of one of the largest families of RNA-binding proteins, the RRM (RNA Recognition Motif) family. The gene has been named hermes (for HEart, RRM Expressed Sequence). The hermes protein is 197-amino acids long and contains a single RRM domain. In situ hybridization analysis indicates that hermes is expressed at highest levels in the myocardium of the heart and to a lesser extent in the ganglion layer of the retina, the pronephros and epiphysis. Expression of hermes in each of these tissues begins at approximately the time of differentiation and is maintained throughout development. Analysis of the RNA expression of the hermes orthologues from chicken and mouse reveals that, like Xenopus, the most prominent tissue of expression is the developing heart. The sequence and expression pattern of hermes suggests a role in post-transcriptional regulation of heart development.
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10096065
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rbpms2 (RNA binding protein with multiple splicing 2) gene expression in Xenopus laevis embryos, NF stage 34, as assayed by in situ hybridization. Lateral view: anteriorleft, dorsal up.
Fig. 3. Whole mount in situ hybridization analysis of embryonic hermes expression in Xenopus . (A) and (F) are whole mount in situs on albino embryos, (E) is a whole mount in situ on a pigmented embryo. (A) Early tailbudembryo (stage 26/27) showing expression of hermes in the heartprimordia (black arrow) and the pronephric region (white arrow). (B) Ventral view of embryo shown in (A), indicating the paired heartprimordia. (C) Stage 28 embryo, showing increase in hermes expression in the heart and pronephros. (D) Stage 36 embryo showing hermes expression in the heart, pronephros, eye and epiphysis (at the top of head). (E) Stage 34 embryo, showing onset of hermes expression in the eye. Note the punctate staining concentrated in the dorsal region of the eye. (F) Dorsal view of a stage 36 embryo showing paired epiphyseal staining on the top of the head. (G) Lateral view of a stage 36 embryo showing duct and tubule staining of the pronephros. (H) Section through the heart region of a stage 31 embryo shows hermes expression is limited to the myocardial layer of the heart and is absent from the endocardium. (I) Section through the anteriorpronephros of a stage 35 embryo. hermes is expressed throughout the epithelium of tubules and glomus of the pronephros. (J) Section through the eye of a stage 36 embryo shows hermes expression in the ganglion layer, the inner-most cellular layer of the retina. E, endocardium; M, myocardium; L, lens; P, pigmented epithelium; T, tubules; G, glomus; S, somite.
