Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1999 Mar 16;966:2817-22. doi: 10.1073/pnas.96.6.2817.
Show Gene links
Show Anatomy links
Cyclin-dependent kinase control of centrosome duplication.
Lacey KR
,
Jackson PK
,
Stearns T
.
???displayArticle.abstract???
Centrosomes nucleate microtubules and duplicate once per cell cycle. This duplication and subsequent segregation in mitosis results in maintenance of the one centrosome/cell ratio. Centrosome duplication occurs during the G1/S transition in somatic cells and must be coupled to the events of the nuclear cell cycle; failure to coordinate duplication and mitosis results in abnormal numbers of centrosomes and aberrant mitoses. Using both in vivo and in vitro assays, we show that centrosome duplication in Xenopus laevis embryos requires cyclin/cdk2 kinase activity. Injection of the cdk (cyclin-dependent kinase) inhibitor p21 into one blastomere of a dividing embryo blocks centrosome duplication in that blastomere; the related cdk inhibitor p27 has a similar effect. An in vitro system using Xenopus extracts carries out separation of the paired centrioles within the centrosome. This centriole separation activity is dependent on cyclin/cdk2 activity; depletion of either cdk2 or of the two activating cyclins, cyclin A and cyclin E, eliminates centriole separation activity. In addition, centriole separation is inhibited by the mitotic state, suggesting a mechanism of linking the cell cycle to periodic duplication of the centrosome.
Balczon,
Dissociation of centrosome replication events from cycles of DNA synthesis and mitotic division in hydroxyurea-arrested Chinese hamster ovary cells.
1995, Pubmed
Balczon,
Dissociation of centrosome replication events from cycles of DNA synthesis and mitotic division in hydroxyurea-arrested Chinese hamster ovary cells.
1995,
Pubmed
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Blow,
Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly.
1990,
Pubmed
,
Xenbase
Blow,
A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts.
1990,
Pubmed
,
Xenbase
Chen,
Separate domains of p21 involved in the inhibition of Cdk kinase and PCNA.
1995,
Pubmed
,
Xenbase
Cohen-Fix,
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
1996,
Pubmed
,
Xenbase
Coverley,
Reversible effects of nuclear membrane permeabilization on DNA replication: evidence for a positive licensing factor.
1993,
Pubmed
,
Xenbase
Dulić,
Association of human cyclin E with a periodic G1-S phase protein kinase.
1992,
Pubmed
Dulić,
p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest.
1994,
Pubmed
Fang,
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.
1991,
Pubmed
,
Xenbase
Fang,
Distinct roles of cdk2 and cdc2 in RP-A phosphorylation during the cell cycle.
1993,
Pubmed
,
Xenbase
Feldman,
A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.
1997,
Pubmed
Fry,
A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators.
1998,
Pubmed
Fry,
C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2.
1998,
Pubmed
Fukasawa,
Abnormal centrosome amplification in the absence of p53.
1996,
Pubmed
Félix,
Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation.
1994,
Pubmed
,
Xenbase
Gard,
Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.
1990,
Pubmed
,
Xenbase
Gu,
Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit.
1993,
Pubmed
Harper,
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
1993,
Pubmed
Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase
Hinchcliffe,
The coordination of centrosome reproduction with nuclear events of the cell cycle in the sea urchin zygote.
1998,
Pubmed
Hinchcliffe,
Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
Jackson,
Early events in DNA replication require cyclin E and are blocked by p21CIP1.
1995,
Pubmed
,
Xenbase
Kellogg,
The centrosome and cellular organization.
1994,
Pubmed
Kochanski,
Mode of centriole duplication and distribution.
1990,
Pubmed
Koff,
Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
1992,
Pubmed
Kung,
Differences in the regulation of protein synthesis, cyclin B accumulation, and cellular growth in response to the inhibition of DNA synthesis in Chinese hamster ovary and HeLa S3 cells.
1993,
Pubmed
Kuriyama,
Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy.
1981,
Pubmed
McDonald,
A proteasome cap subunit required for spindle pole body duplication in yeast.
1997,
Pubmed
Meijer,
Cyclic activation of histone H1 kinase during sea urchin egg mitotic divisions.
1988,
Pubmed
Mitchison,
Isolation of mammalian centrosomes.
1986,
Pubmed
Morgan,
Production of p60c-src by baculovirus expression and immunoaffinity purification.
1991,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Neant,
6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity.
1988,
Pubmed
Paintrand,
Centrosome organization and centriole architecture: their sensitivity to divalent cations.
1992,
Pubmed
Palazzo,
Centriole duplication in lysates of Spisula solidissima oocytes.
1992,
Pubmed
Polyak,
p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.
1994,
Pubmed
Polyak,
Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.
1994,
Pubmed
Reinsch,
Movement of nuclei along microtubules in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Rempel,
Maternal Xenopus Cdk2-cyclin E complexes function during meiotic and early embryonic cell cycles that lack a G1 phase.
1995,
Pubmed
,
Xenbase
Schwob,
The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.
1994,
Pubmed
Sluder,
Protein synthesis and the cell cycle: centrosome reproduction in sea urchin eggs is not under translational control.
1990,
Pubmed
Stearns,
In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.
1994,
Pubmed
,
Xenbase
Strausfeld,
Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Strausfeld,
Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases.
1994,
Pubmed
,
Xenbase
Tournier,
Centrosomes competent for parthenogenesis in Xenopus eggs support procentriole budding in cell-free extracts.
1991,
Pubmed
,
Xenbase
Toyoshima,
p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21.
1994,
Pubmed
Winey,
Assembly and functions of the spindle pole body in budding yeast.
1993,
Pubmed
Xiong,
p21 is a universal inhibitor of cyclin kinases.
1993,
Pubmed
el-Deiry,
WAF1, a potential mediator of p53 tumor suppression.
1993,
Pubmed
