Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nerve terminal currents induced by autoreception of acetylcholine release.
Fu WM
,
Liou HC
,
Chen YH
.
???displayArticle.abstract???
The activation of autoreceptors is known to be important in the modulation of presynaptic transmitter secretion in peripheral and central neurons. Using whole-cell recordings made from the free growth cone of myocyte-contact motoneurons of Xenopus cell cultures, we have observed spontaneous nerve terminal currents (NTCs). These spontaneous NTCs are blocked by d-tubocurarine (d-TC) and alpha-bungarotoxin (alpha-BuTx), indicating that endogenously released acetylcholine (ACh) can produce substantial membrane depolarization in the nerve terminals. Local application of NMDA to the growth cone increased the frequency of spontaneous NTCs. When the electrical stimulations were applied at the soma to initiate evoked-release of ACh, evoked ACh-induced potentials were recorded in the nerve terminals, which were inhibited by d-TC and hexamethonium but not by atropine. Replacement of normal Ringer's solution with high-Mg2+, low-Ca2+ solution also reversibly inhibited evoked ACh-induced potentials. The possible regulatory role of presynaptic nicotinic autoreceptors on the synaptic transmission was also examined. When the innervated myocyte was whole-cell voltage-clamped to record synaptic currents, application of hexamethonium inhibited the amplitude of evoked synaptic currents at a higher degree than that of iontophoretic ACh-induced currents. Furthermore, hexamethonium markedly reduced the frequency of spontaneous synaptic currents at high-activity synapses. Pretreatment of neurons with alpha-BuTx also inhibited the evoked synaptic currents in manipulated synapses. These results suggest that ACh released spontaneously or by electrical stimulation may act on the presynaptic nicotinic autoreceptors of the same nerve terminals to produce membrane potential change and to regulate synaptic transmission.
Araujo,
Characterization of N-[3H]methylcarbamylcholine binding sites and effect of N-methylcarbamylcholine on acetylcholine release in rat brain.
1988, Pubmed
Araujo,
Characterization of N-[3H]methylcarbamylcholine binding sites and effect of N-methylcarbamylcholine on acetylcholine release in rat brain.
1988,
Pubmed
Boehm,
Inhibition of N-type calcium channels: the only mechanism by which presynaptic alpha 2-autoreceptors control sympathetic transmitter release.
1996,
Pubmed
Bowman,
Feedback control of transmitter release at the neuromuscular junction.
1988,
Pubmed
Chan,
A role for the nicotinic alpha-bungarotoxin receptor in neurite outgrowth in PC12 cells.
1993,
Pubmed
Chow,
Release of acetylcholine from embryonic neurons upon contact with muscle cell.
1985,
Pubmed
,
Xenbase
Clarke,
Nicotinic receptors in mammalian brain: localization and relation to cholinergic innervation.
1993,
Pubmed
Coggan,
Direct recording of nicotinic responses in presynaptic nerve terminals.
1997,
Pubmed
Couturier,
A neuronal nicotinic acetylcholine receptor subunit (alpha 7) is developmentally regulated and forms a homo-oligomeric channel blocked by alpha-BTX.
1990,
Pubmed
,
Xenbase
Cragg,
Differential autoreceptor control of somatodendritic and axon terminal dopamine release in substantia nigra, ventral tegmental area, and striatum.
1997,
Pubmed
Fu,
Potentiation of neurotransmitter release by activation of presynaptic glutamate receptors at developing neuromuscular synapses of Xenopus.
1995,
Pubmed
,
Xenbase
Fu,
Regulation of acetylcholine release by presynaptic nicotinic receptors at developing neuromuscular synapses.
1997,
Pubmed
,
Xenbase
Fu,
Release of acetylcholine from embryonic myocytes in Xenopus cell cultures.
1998,
Pubmed
,
Xenbase
Fu,
L-type Ca2+ channel is involved in the regulation of spontaneous transmitter release at developing neuromuscular synapses.
1994,
Pubmed
,
Xenbase
Gibb,
Pre-and post-junctional effects of tubocurarine and other nicotinic antagonists during repetitive stimulation in the rat.
1984,
Pubmed
Gray,
Hippocampal synaptic transmission enhanced by low concentrations of nicotine.
1996,
Pubmed
Greene,
Synthesis, storage and release of acetylcholine by a noradrenergic pheochromocytoma cell line.
1977,
Pubmed
Guo,
Glutamate and GABA release are enhanced by different subtypes of presynaptic nicotinic receptors in the lateral geniculate nucleus.
1998,
Pubmed
Göthert,
Presynaptic 5-HT auto- and heteroreceptors in the human central and peripheral nervous system.
1996,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Jacob,
The ultrastructural localization of alpha-bungarotoxin binding sites in relation to synapses on chick ciliary ganglion neurons.
1983,
Pubmed
Levin,
Nicotinic systems and cognitive function.
1992,
Pubmed
Li,
Presynaptic nicotinic receptors facilitate monoaminergic transmission.
1998,
Pubmed
Lindstrom,
Neuronal nicotinic acetylcholine receptors.
1996,
Pubmed
Lipton,
Spontaneous release of acetylcholine affects the physiological nicotinic responses of rat retinal ganglion cells in culture.
1988,
Pubmed
Lipton,
Nicotinic antagonists enhance process outgrowth by rat retinal ganglion cells in culture.
1988,
Pubmed
Macek,
Differential involvement of group II and group III mGluRs as autoreceptors at lateral and medial perforant path synapses.
1996,
Pubmed
Magleby,
The effect of (+)-tubocurarine on neuromuscular transmission during repetitive stimulation in the rat, mouse, and frog.
1981,
Pubmed
McGehee,
Nicotine enhancement of fast excitatory synaptic transmission in CNS by presynaptic receptors.
1995,
Pubmed
McGehee,
Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons.
1995,
Pubmed
Misgeld,
A physiological role for GABAB receptors and the effects of baclofen in the mammalian central nervous system.
1995,
Pubmed
Nordberg,
The role of nicotinic receptors in the pathophysiology of Alzheimer's disease.
1989,
Pubmed
Pugh,
Neuronal acetylcholine receptors that bind alpha-bungarotoxin mediate neurite retraction in a calcium-dependent manner.
1994,
Pubmed
Quik,
Thymopoietin interacts at the alpha-bungarotoxin site of and induces process formation in PC12 pheochromocytoma cells.
1990,
Pubmed
Ramirez-Latorre,
Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.
1996,
Pubmed
,
Xenbase
Role,
Nicotinic receptors in the development and modulation of CNS synapses.
1996,
Pubmed
Rowell,
Nicotinic stimulation of [3H]acetylcholine release from mouse cerebral cortical synaptosomes.
1984,
Pubmed
Sargent,
The diversity of neuronal nicotinic acetylcholine receptors.
1993,
Pubmed
Schoepfer,
Brain alpha-bungarotoxin binding protein cDNAs and MAbs reveal subtypes of this branch of the ligand-gated ion channel gene superfamily.
1990,
Pubmed
Smith,
Characterization of a component in chick ciliary ganglia that cross-reacts with monoclonal antibodies to muscle and electric organ acetylcholine receptor.
1985,
Pubmed
Starke,
Modulation of neurotransmitter release by presynaptic autoreceptors.
1989,
Pubmed
Séguéla,
Molecular cloning, functional properties, and distribution of rat brain alpha 7: a nicotinic cation channel highly permeable to calcium.
1993,
Pubmed
,
Xenbase
Vijayaraghavan,
Nicotinic receptors that bind alpha-bungarotoxin on neurons raise intracellular free Ca2+.
1992,
Pubmed
Wang,
Assembly of human neuronal nicotinic receptor alpha5 subunits with alpha3, beta2, and beta4 subunits.
1996,
Pubmed
,
Xenbase
Wessler,
Acetylcholine at motor nerves: storage, release, and presynaptic modulation by autoreceptors and adrenoceptors.
1992,
Pubmed
Wilkie,
Pharmacological characterization of a nicotinic autoreceptor in rat hippocampal synaptosomes.
1996,
Pubmed
Wonnacott,
Presynaptic modulation of transmitter release by nicotinic receptors.
1989,
Pubmed