Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1998 Oct 27;9522:12906-11. doi: 10.1073/pnas.95.22.12906.
Show Gene links
Show Anatomy links
Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.
Schmiesing JA
,
Ball AR
,
Gregson HC
,
Alderton JM
,
Zhou S
,
Yokomori K
.
???displayArticle.abstract???
The structural maintenance of chromosomes (SMC) family member proteins previously were shown to play a critical role in mitotic chromosome condensation and segregation in yeast and Xenopus. Other family members were demonstrated to be required for DNA repair in yeast and mammals. Although several different SMC proteins were identified in different organisms, little is known about the SMC proteins in humans. Here, we report the identification of four human SMC proteins that form two distinct heterodimeric complexes in the cell, the human chromosome-associated protein (hCAP)-C and hCAP-E protein complex (hCAP-C/hCAP-E), and the human SMC1 (hSMC1) and hSMC3 protein complex (hSMC1/hSMC3). The hCAP-C/hCAP-E complex is the human ortholog of the Xenopus chromosome-associated protein (XCAP)-C/XCAP-E complex required for mitotic chromosome condensation. We found that a second complex, hSMC1/hSMC3, is required for metaphase progression in mitotic cells. Punctate vs. diffuse distribution patterns of the hCAP-C/hCAP-E and hSMC1/hSMC3 complexes in the interphase nucleus indicate independent behaviors of the two complexes during the cell cycle. These results suggest that two distinct classes of SMC protein complexes are involved in different aspects of mitotic chromosome organization in human cells.
Chuang,
DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.
1994, Pubmed,
Xenbase
Chuang,
DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome.
1994,
Pubmed
,
Xenbase
Earnshaw,
Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome.
1985,
Pubmed
Gasser,
Chromosome structure. Coiling up chromosomes.
1995,
Pubmed
Guacci,
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.
1997,
Pubmed
Heck,
Condensins, cohesins, and chromosome architecture: how to make and break a mitotic chromosome.
1997,
Pubmed
Hirano,
The SMC family: from chromosome condensation to dosage compensation.
1995,
Pubmed
,
Xenbase
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Jessberger,
SMC proteins constitute two subunits of the mammalian recombination complex RC-1.
1996,
Pubmed
,
Xenbase
Jessberger,
Chromosome dynamics: the SMC protein family.
1998,
Pubmed
Koshland,
Mitotic chromosome condensation.
1996,
Pubmed
Lehmann,
The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair.
1995,
Pubmed
Lieb,
MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.
1998,
Pubmed
Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase
Michaelis,
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
1997,
Pubmed
Peterson,
The SMC family: novel motor proteins for chromosome condensation?
1994,
Pubmed
Rocques,
The human SB1.8 gene (DXS423E) encodes a putative chromosome segregation protein conserved in lower eukaryotes and prokaryotes.
1995,
Pubmed
Saitoh,
The SMC proteins and the coming of age of the chromosome scaffold hypothesis.
1995,
Pubmed
,
Xenbase
Saitoh,
ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.
1994,
Pubmed
Saka,
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
1994,
Pubmed
Shimizu,
Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide.
1998,
Pubmed
,
Xenbase
Strunnikov,
SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.
1993,
Pubmed
Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed
Yokomori,
Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.
1993,
Pubmed
