Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein.
Kanamori H
,
Dodson RE
,
Shapiro DJ
.
???displayArticle.abstract???
The function(s) and RNA binding properties of vigilin, a ubiquitous protein with 14 KH domains, remain largely obscure. We recently showed that vigilin is the estrogen-inducible protein in polysome extracts which binds specifically to a segment of the 3' untranslated region (UTR) of estrogen-stabilized vitellogenin mRNA. In order to identify consensus mRNA sequences and structures important in binding of vigilin to RNA, before vigilin was purified, we developed a modified in vitro genetic selection protocol. We subsequently validated our selection procedure, which employed crude polysome extracts, by testing natural and in vitro-selected RNAs with purified recombinant vigilin. Most of the selected up-binding mutants exhibited hypermutation of G residues leading to a largely unstructured, single-stranded region containing multiple conserved (A)nCU and UC(A)n motifs. All eight of the selected down-binding mutants contained a mutation in the sequence (A)nCU. Deletion analysis indicated that approximately 75 nucleotides are required for maximal binding. Using this information, we predicted and subsequently identified a strong vigilin binding site near the 3' end of human dystrophin mRNA. RNA sequences from the 3' UTRs of transferrin receptor and estrogen receptor, which lack strong homology to the selected sequences, did not bind vigilin. These studies describe an aproach to identifying long RNA binding sites and describe sequence and structural requirements for interaction of vigilin with RNAs.
Arning,
Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA.
1996, Pubmed
Arning,
Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA.
1996,
Pubmed
Ashley,
FMR1 protein: conserved RNP family domains and selective RNA binding.
1993,
Pubmed
Bartel,
HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA.
1991,
Pubmed
Battiste,
Binding of an HIV Rev peptide to Rev responsive element RNA induces formation of purine-purine base pairs.
1994,
Pubmed
Beelman,
Degradation of mRNA in eukaryotes.
1995,
Pubmed
Binkley,
RNA ligands to human nerve growth factor.
1995,
Pubmed
Blackwell,
Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection.
1990,
Pubmed
Blume,
Ribosome loading, but not protein synthesis, is required for estrogen stabilization of Xenopus laevis vitellogenin mRNA.
1989,
Pubmed
,
Xenbase
Brock,
Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation.
1983,
Pubmed
,
Xenbase
Brown,
Single-stranded RNA recognition by the bacteriophage T4 translational repressor, regA.
1997,
Pubmed
Buckanovich,
The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies.
1996,
Pubmed
Buckanovich,
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
1997,
Pubmed
Burd,
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
1994,
Pubmed
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Cadwell,
Randomization of genes by PCR mutagenesis.
1992,
Pubmed
Casey,
Iron regulation of transferrin receptor mRNA levels requires iron-responsive elements and a rapid turnover determinant in the 3' untranslated region of the mRNA.
1989,
Pubmed
Chagnovich,
Differential activity of ELAV-like RNA-binding proteins in human neuroblastoma.
1996,
Pubmed
Chen,
AU-rich elements: characterization and importance in mRNA degradation.
1995,
Pubmed
Chiang,
Expression and purification of general transcription factors by FLAG epitope-tagging and peptide elution.
1993,
Pubmed
DeMaria,
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
1996,
Pubmed
Dejgaard,
Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains.
1996,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Dodson,
An estrogen-inducible protein binds specifically to a sequence in the 3' untranslated region of estrogen-stabilized vitellogenin mRNA.
1994,
Pubmed
,
Xenbase
Dodson,
Tissue distribution, hormone regulation and evidence for a human homologue of the estrogen-inducible Xenopus laevis vitellogenin mRNA binding protein.
1995,
Pubmed
,
Xenbase
Dodson,
Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein.
1997,
Pubmed
,
Xenbase
Gibson,
The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid.
1993,
Pubmed
Gold,
Diversity of oligonucleotide functions.
1995,
Pubmed
Green,
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
,
Pubmed
Görlach,
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
1994,
Pubmed
Heinrichs,
The Drosophila SR protein RBP1 contributes to the regulation of doublesex alternative splicing by recognizing RBP1 RNA target sequences.
1995,
Pubmed
Jellinek,
High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding.
1993,
Pubmed
Kjems,
Specific binding of a basic peptide from HIV-1 Rev.
1992,
Pubmed
Kruse,
Evidence for a novel cytoplasmic tRNA-protein complex containing the KH-multidomain protein vigilin.
1996,
Pubmed
Kügler,
Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus.
1996,
Pubmed
Kühn,
Gene transfer, expression, and molecular cloning of the human transferrin receptor gene.
1984,
Pubmed
Lacadena,
Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.
1994,
Pubmed
Leffers,
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
1995,
Pubmed
Levine,
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
1993,
Pubmed
Liu,
The product of the pleiotropic Escherichia coli gene csrA modulates glycogen biosynthesis via effects on mRNA stability.
1995,
Pubmed
Lorincz,
Monoclonal antibody recognition of multiple forms of estrogen receptor tagged with [125I]methoxy-iodovinyl estradiol in ovarian carcinomas.
1985,
Pubmed
Matunis,
Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.
1992,
Pubmed
McKnight,
Cloning and expression of a cellular high density lipoprotein-binding protein that is up-regulated by cholesterol loading of cells.
1992,
Pubmed
Musco,
Three-dimensional structure and stability of the KH domain: molecular insights into the fragile X syndrome.
1996,
Pubmed
Müllner,
A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA.
1989,
Pubmed
Nandabalan,
Binding of a cell-type-specific RNA splicing factor to its target regulatory sequence.
1995,
Pubmed
Neu-Yilik,
Vigilin is a cytoplasmic protein. A study on its expression in primary cells and in established cell lines of different species.
1993,
Pubmed
Nielsen,
Estradiol and estrogen receptor-dependent stabilization of a minivitellogenin mRNA lacking 5,100 nucleotides of coding sequence.
1990,
Pubmed
,
Xenbase
Plenz,
The human vigilin gene: identification, chromosomal localization and expression pattern.
1994,
Pubmed
Plenz,
Expression of vigilin in chicken cartilage and bone.
1993,
Pubmed
Ree,
Down-regulation of messenger ribonucleic acid (mRNA) for the estrogen receptor (ER) by phorbol ester requires ongoing RNA synthesis but not protein synthesis. Is hormonal control of ER mRNA degradation mediated by an RNA molecule?
1992,
Pubmed
Ross,
mRNA stability in mammalian cells.
1995,
Pubmed
Saceda,
Post-transcriptional destabilization of estrogen receptor mRNA in MCF-7 cells by 12-O-tetradecanoylphorbol-13-acetate.
1991,
Pubmed
Schmidt,
Complete cDNA sequence of chicken vigilin, a novel protein with amplified and evolutionary conserved domains.
1992,
Pubmed
Siebel,
Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing.
1995,
Pubmed
Singh,
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
1995,
Pubmed
Siomi,
Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome.
1994,
Pubmed
Siomi,
FXR1, an autosomal homolog of the fragile X mental retardation gene.
1995,
Pubmed
,
Xenbase
Siomi,
Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them.
1996,
Pubmed
Siomi,
The pre-mRNA binding K protein contains a novel evolutionarily conserved motif.
1993,
Pubmed
,
Xenbase
Swanson,
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
1988,
Pubmed
Tacke,
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
1995,
Pubmed
Takagaki,
RNA recognition by the human polyadenylation factor CstF.
1997,
Pubmed
Tsai,
U1-snRNP-A protein selects a ten nucleotide consensus sequence from a degenerate RNA pool presented in various structural contexts.
1991,
Pubmed
Tuerk,
Autogenous translational operator recognized by bacteriophage T4 DNA polymerase.
1990,
Pubmed
Wang,
P62 association with RNA is regulated by tyrosine phosphorylation.
1995,
Pubmed
Wang,
Complementary change in cis determinants and trans factors in the evolution of an mRNP stability complex.
1996,
Pubmed
Wang,
Sex-lethal interactions with protein and RNA. Roles of glycine-rich and RNA binding domains.
1997,
Pubmed
Wintersberger,
Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintenance of exact ploidy.
1995,
Pubmed
Zuker,
Computer prediction of RNA structure.
1989,
Pubmed
