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Mol Cell Biol
1998 Jun 01;186:3405-15. doi: 10.1128/MCB.18.6.3405.
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GATA-4 and Nkx-2.5 coactivate Nkx-2 DNA binding targets: role for regulating early cardiac gene expression.
Sepulveda JL
,
Belaguli N
,
Nigam V
,
Chen CY
,
Nemer M
,
Schwartz RJ
.
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The cardiogenic homeodomain factor Nkx-2.5 and serum response factor (SRF) provide strong transcriptional coactivation of the cardiac alpha-actin (alphaCA) promoter in fibroblasts (C. Y. Chen and R. J. Schwartz, Mol. Cell. Biol. 16:6372-6384, 1996). We demonstrate here that Nkx-2.5 also cooperates with GATA-4, a dual C-4 zinc finger transcription factor expressed in early cardiac progenitor cells, to activate the alphaCA promoter and a minimal promoter, containing only multimerized Nkx-2.5 DNA binding sites (NKEs), in heterologous CV-1 fibroblasts. Transcriptional activity requires the N-terminal activation domain of Nkx-2.5 and Nkx-2.5 binding activity through its homeodomain but does not require GATA-4's activation domain. The minimal interactive regions were mapped to the homeodomain of Nkx-2.5 and the second zinc finger of GATA-4. Removal of Nkx-2.5's C-terminal inhibitory domain stimulated robust transcriptional activity, comparable to the effects of GATA-4 on wild-type Nkx-2.5, which in part facilitated Nkx-2.5 DNA binding activity. We postulate the following simple model: GATA-4 induces a conformational change in Nkx-2.5 that displaces the C-terminal inhibitory domain, thus eliciting transcriptional activation of promoters containing Nkx-2.5 DNA binding targets. Therefore, alphaCa promoter activity appears to be regulated through the combinatorial interactions of at least three cardiac tissue-enriched transcription factors, Nkx-2.5, GATA-4, and SRF.
Azpiazu,
tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
1993, Pubmed
Azpiazu,
tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
1993,
Pubmed
Belaguli,
Organization and myogenic restricted expression of the murine serum response factor gene. A role for autoregulation.
1997,
Pubmed
,
Xenbase
Bodmer,
The gene tinman is required for specification of the heart and visceral muscles in Drosophila.
1993,
Pubmed
Bohinski,
The lung-specific surfactant protein B gene promoter is a target for thyroid transcription factor 1 and hepatocyte nuclear factor 3, indicating common factors for organ-specific gene expression along the foregut axis.
1994,
Pubmed
Brazas,
Determining the requirements for cooperative DNA binding by Swi5p and Pho2p (Grf10p/Bas2p) at the HO promoter.
1995,
Pubmed
Brazas,
Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein.
1993,
Pubmed
Brazas,
The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter.
1993,
Pubmed
Chen,
Identification of novel DNA binding targets and regulatory domains of a murine tinman homeodomain factor, nkx-2.5.
1995,
Pubmed
,
Xenbase
Chen,
Recruitment of the tinman homolog Nkx-2.5 by serum response factor activates cardiac alpha-actin gene transcription.
1996,
Pubmed
,
Xenbase
Chen,
Activation of the cardiac alpha-actin promoter depends upon serum response factor, Tinman homologue, Nkx-2.5, and intact serum response elements.
1996,
Pubmed
,
Xenbase
Chen,
Zebrafish tinman homolog demarcates the heart field and initiates myocardial differentiation.
1996,
Pubmed
,
Xenbase
Chen,
Competition between negative acting YY1 versus positive acting serum response factor and tinman homologue Nkx-2.5 regulates cardiac alpha-actin promoter activity.
1997,
Pubmed
,
Xenbase
Civitareale,
A thyroid-specific nuclear protein essential for tissue-specific expression of the thyroglobulin promoter.
1989,
Pubmed
Croissant,
Avian serum response factor expression restricted primarily to muscle cell lineages is required for alpha-actin gene transcription.
1996,
Pubmed
Crossley,
Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains.
1995,
Pubmed
Damante,
Sequence-specific DNA recognition by the thyroid transcription factor-1 homeodomain.
1994,
Pubmed
Dawid,
LIM domain proteins.
1995,
Pubmed
Dohrmann,
Role of negative regulation in promoter specificity of the homologous transcriptional activators Ace2p and Swi5p.
1996,
Pubmed
Durocher,
The atrial natriuretic factor promoter is a downstream target for Nkx-2.5 in the myocardium.
1996,
Pubmed
,
Xenbase
Durocher,
The cardiac transcription factors Nkx2-5 and GATA-4 are mutual cofactors.
1997,
Pubmed
,
Xenbase
Fischer,
Cooperation of GATA-1 and Sp1 can result in synergistic transcriptional activation or interference.
1993,
Pubmed
Gajewski,
D-mef2 is a target for Tinman activation during Drosophila heart development.
1997,
Pubmed
Gregory,
Functional interaction of GATA1 with erythroid Krüppel-like factor and Sp1 at defined erythroid promoters.
1996,
Pubmed
Grépin,
A hormone-encoding gene identifies a pathway for cardiac but not skeletal muscle gene transcription.
1994,
Pubmed
,
Xenbase
Guazzi,
Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity.
1990,
Pubmed
Guichet,
The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors.
1997,
Pubmed
Harvey,
NK-2 homeobox genes and heart development.
1996,
Pubmed
Ido,
Activation of ATBF1, a multiple-homeodomain zinc-finger gene, during neuronal differentiation of murine embryonal carcinoma cells.
1994,
Pubmed
Ip,
The GATA-4 transcription factor transactivates the cardiac muscle-specific troponin C promoter-enhancer in nonmuscle cells.
1994,
Pubmed
Jiang,
The Xenopus GATA-4/5/6 genes are associated with cardiac specification and can regulate cardiac-specific transcription during embryogenesis.
1996,
Pubmed
,
Xenbase
Kawana,
Cooperative interaction of GATA-2 and AP1 regulates transcription of the endothelin-1 gene.
1995,
Pubmed
Knoepfler,
The pentapeptide motif of Hox proteins is required for cooperative DNA binding with Pbx1, physically contacts Pbx1, and enhances DNA binding by Pbx1.
1995,
Pubmed
Knoepfler,
Pbx-1 Hox heterodimers bind DNA on inseparable half-sites that permit intrinsic DNA binding specificity of the Hox partner at nucleotides 3' to a TAAT motif.
1996,
Pubmed
Ko,
DNA-binding specificities of the GATA transcription factor family.
1993,
Pubmed
Kobayashi,
Cooperative interaction between AhR.Arnt and Sp1 for the drug-inducible expression of CYP1A1 gene.
1996,
Pubmed
Komuro,
Csx: a murine homeobox-containing gene specifically expressed in the developing heart.
1993,
Pubmed
Kuo,
Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations.
1997,
Pubmed
Kuo,
GATA4 transcription factor is required for ventral morphogenesis and heart tube formation.
1997,
Pubmed
Kutoh,
Functional interference between the ubiquitous and constitutive octamer transcription factor 1 (OTF-1) and the glucocorticoid receptor by direct protein-protein interaction involving the homeo subdomain of OTF-1.
1992,
Pubmed
Lai,
Loss of function of the Drosophila zfh-1 gene results in abnormal development of mesodermally derived tissues.
1993,
Pubmed
Laverriere,
GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut.
1994,
Pubmed
Lee,
Displacement of BrdUrd-induced YY1 by serum response factor activates skeletal alpha-actin transcription in embryonic myoblasts.
1992,
Pubmed
Lee,
A new tinman-related gene, nkx2.7, anticipates the expression of nkx2.5 and nkx2.3 in zebrafish heart and pharyngeal endoderm.
1996,
Pubmed
,
Xenbase
Lints,
Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants.
1993,
Pubmed
Merika,
Functional synergy and physical interactions of the erythroid transcription factor GATA-1 with the Krüppel family proteins Sp1 and EKLF.
1995,
Pubmed
Merika,
DNA-binding specificity of GATA family transcription factors.
1993,
Pubmed
Molkentin,
Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis.
1997,
Pubmed
Molkentin,
Transcription factor GATA-4 regulates cardiac muscle-specific expression of the alpha-myosin heavy-chain gene.
1994,
Pubmed
Morrisey,
GATA-6: a zinc finger transcription factor that is expressed in multiple cell lineages derived from lateral mesoderm.
1996,
Pubmed
Morrisey,
GATA-5: a transcriptional activator expressed in a novel temporally and spatially-restricted pattern during embryonic development.
1997,
Pubmed
Ray,
Transcriptional regulation of a mouse Clara cell-specific protein (mCC10) gene by the NKx transcription factor family members thyroid transciption factor 1 and cardiac muscle-specific homeobox protein (CSX).
1996,
Pubmed
Reecy,
Chicken Nkx-2.8: a novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm.
1997,
Pubmed
Sartorelli,
Molecular mechanisms of myogenic coactivation by p300: direct interaction with the activation domain of MyoD and with the MADS box of MEF2C.
1997,
Pubmed
Schultheiss,
Induction of avian cardiac myogenesis by anterior endoderm.
1995,
Pubmed
,
Xenbase
Sharrocks,
DNA bending in the ternary nucleoprotein complex at the c-fos promoter.
1995,
Pubmed
Thuerauf,
Regulation of rat brain natriuretic peptide transcription. A potential role for GATA-related transcription factors in myocardial cell gene expression.
1994,
Pubmed
Tonissen,
XNkx-2.5, a Xenopus gene related to Nkx-2.5 and tinman: evidence for a conserved role in cardiac development.
1994,
Pubmed
,
Xenbase
Toonen,
The lung enriched transcription factor TTF-1 and the ubiquitously expressed proteins Sp1 and Sp3 interact with elements located in the minimal promoter of the rat Clara cell secretory protein gene.
1996,
Pubmed
Uetz,
Vectors for expression of protein-A-tagged proteins in vertebrate cells.
1996,
Pubmed
Vershon,
Protein interactions of homeodomain proteins.
1996,
Pubmed
Wilson,
Homeodomain proteins. Cooperating to be different.
1995,
Pubmed
Wolberger,
Homeodomain interactions.
1996,
Pubmed
Yang,
Distinct roles for the two cGATA-1 finger domains.
1992,
Pubmed
Zappavigna,
Specificity of HOX protein function depends on DNA-protein and protein-protein interactions, both mediated by the homeo domain.
1994,
Pubmed
