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Androgens can rescue axotomized motor neurons from cell death. Here we examine a possible mechanism for this trophic action in juvenile Xenopus laevis: regulation of a calcium-binding protein, calbindin, after axotomy. Western analysis revealed that a monoclonal antibody to calbindin D specifically recognizes a single approximately 28 kDa band in X. laevis CNS and rat cerebellum. Retrograde transport of peroxidase combined with immunohistochemistry demonstrated that somata, axons, and synaptic terminals of laryngeal motor neurons in nucleus (N.) IX-X of X. laevis are calbindin-positive. The number of calbindin-positive cells was compared in the intact and axotomized sides of N.IX-X of gonadectomized males that were either hormonally untreated or DHT-treated for 1 month. Although axotomy decreased the number of calbindin-positive cells by 86% in hormonally untreated males, the decrease was only 56% in DHT-treated animals. Compared with hormonally untreated animals, the number of calbindin-labeled cells in N.IX-X of DHT-treated males was increased in both the intact (14%) and axotomized sides (75%). We conclude that axotomy decreases and that DHT enhances calbindin immunoreactivity in N.IX-X. Axotomy-induced decrease in calbindin immunoreactivity precedes cell loss in N.IX-X and may impair the capacity of motor neurons to regulate cytoplasmic calcium. Androgen-mediated maintenance of calbindin expression is thus a candidate cellular mechanism for trophic maintenance of hormone target neurons.
ABERCROMBIE,
Estimation of nuclear population from microtome sections.
1946, Pubmed
ABERCROMBIE,
Estimation of nuclear population from microtome sections.
1946,
Pubmed
BARR,
A quantitative study of certain morphological changes in spinal motor neurons during axon reaction.
1948,
Pubmed
Baimbridge,
Calcium-binding proteins in the nervous system.
1992,
Pubmed
Beato,
Steroid hormone receptors: many actors in search of a plot.
1995,
Pubmed
Berger,
Androgen-regulated gene expression.
1989,
Pubmed
Blaustein,
Calcium transport and buffering in neurons.
1988,
Pubmed
Breedlove,
Sexual dimorphism in the vertebrate nervous system.
1992,
Pubmed
Chard,
Regulation of excitatory transmission at hippocampal synapses by calbindin D28k.
1995,
Pubmed
Collazo,
Cellular targets and trophic functions of neurotrophin-3 in the developing rat hippocampus.
1992,
Pubmed
Dutar,
Loss of Calbindin-28K Immunoreactivity in Hippocampal Slices from Aged Rats: a Role for Calcium?
1991,
Pubmed
Ehlers,
Inactivation of NMDA receptors by direct interaction of calmodulin with the NR1 subunit.
1996,
Pubmed
Garcia-Segura,
Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system.
1984,
Pubmed
George,
Axotomy-induced axonal degeneration is mediated by calcium influx through ion-specific channels.
1995,
Pubmed
Hauser,
Androgen increases the number of cells in fetal mouse spinal cord cultures: implications for motoneuron survival.
1989,
Pubmed
Ho,
Expression of calbindin-D28K in motoneuron hybrid cells after retroviral infection with calbindin-D28K cDNA prevents amyotrophic lateral sclerosis IgG-mediated cytotoxicity.
1996,
Pubmed
Iacopino,
Corticosterone regulates calbindin-D28k mRNA and protein levels in rat hippocampus.
1990,
Pubmed
Iacopino,
Specific reduction of calcium-binding protein (28-kilodalton calbindin-D) gene expression in aging and neurodegenerative diseases.
1990,
Pubmed
Jande,
Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain.
1981,
Pubmed
Jones,
Androgenic regulation of tubulin gene expression in axotomized hamster facial motoneurons.
1994,
Pubmed
Kelley,
Locations of androgen-concentrating cells in the brain of Xenopus laevis: autoradiography with 3H-dihydrotestosterone.
1981,
Pubmed
,
Xenbase
Kelley,
Autoradiographic localization of hormone-concentrating cells in the brain of an amphibian, Xenopus laevis. I. Testosterone.
1975,
Pubmed
,
Xenbase
Kujawa,
Testosterone differentially regulates the regenerative properties of injured hamster facial motoneurons.
1991,
Pubmed
Köhr,
Endogenous intracellular calcium buffering and the activation/inactivation of HVA calcium currents in rat dentate gyrus granule cells.
1991,
Pubmed
Lieberman,
The axon reaction: a review of the principal features of perikaryal responses to axon injury.
1971,
Pubmed
Marty,
Brain-derived neurotrophic factor promotes the differentiation of various hippocampal nonpyramidal neurons, including Cajal-Retzius cells, in organotypic slice cultures.
1996,
Pubmed
Mattson,
Brain injury and tumor necrosis factors induce calbindin D-28k in astrocytes: evidence for a cytoprotective response.
1995,
Pubmed
Mattson,
Evidence for calcium-reducing and excito-protective roles for the calcium-binding protein calbindin-D28k in cultured hippocampal neurons.
1991,
Pubmed
Muñoz,
Anuran dorsal column nucleus: organization, immunohistochemical characterization, and fiber connections in Rana perezi and Xenopus laevis.
1995,
Pubmed
,
Xenbase
Nordeen,
Androgens prevent normally occurring cell death in a sexually dimorphic spinal nucleus.
1985,
Pubmed
Peterson,
Fibroblast growth factor-2 protects entorhinal layer II glutamatergic neurons from axotomy-induced death.
1996,
Pubmed
Pérez,
Trophic effects of androgen: receptor expression and the survival of laryngeal motor neurons after axotomy.
1996,
Pubmed
,
Xenbase
Pérez,
Androgen receptor mRNA expression in Xenopus laevis CNS: sexual dimorphism and regulation in laryngeal motor nucleus.
1996,
Pubmed
,
Xenbase
Romagnolo,
Tissue-specific and hormonal regulation of calbindin-D9K fusion genes in transgenic mice.
1996,
Pubmed
Sanna,
Presumptive Renshaw cells contain decreased calbindin during recovery from sciatic nerve lesions.
1993,
Pubmed
Simpson,
Origin and identification of fibers in the cranial nerve IX-X complex of Xenopus laevis: Lucifer Yellow backfills in vitro.
1986,
Pubmed
,
Xenbase
Sloviter,
Calcium-binding protein (calbindin-D28k) and parvalbumin immunocytochemistry: localization in the rat hippocampus with specific reference to the selective vulnerability of hippocampal neurons to seizure activity.
1989,
Pubmed
Sánchez-Vives,
Axotomy-induced changes in Ca2+ homeostasis in rat sympathetic ganglion cells.
1994,
Pubmed
Séquier,
Calbindin D-28k Protein and mRNA Localization in the Rat Brain.
1990,
Pubmed
Umemiya,
Electrophysiological properties of axotomized facial motoneurones that are destined to die in neonatal rats.
1993,
Pubmed
Ventimiglia,
The neurotrophins BDNF, NT-3 and NT-4/5 promote survival and morphological and biochemical differentiation of striatal neurons in vitro.
1995,
Pubmed
Wetzel,
A proposed neural pathway for vocalization in South African clawed frogs, Xenopus laevis.
1985,
Pubmed
,
Xenbase
Winsky,
Antibody recognition of calcium-binding proteins depends on their calcium-binding status.
1996,
Pubmed
Yu,
Administration of testosterone attenuates neuronal loss following axotomy in the brain-stem motor nuclei of female rats.
1989,
Pubmed
Yu,
Effect of testosterone on the regeneration of the hypoglossal nerve in rats.
1982,
Pubmed
