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Mol Cell Biol
1997 Mar 01;173:1434-40. doi: 10.1128/MCB.17.3.1434.
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Phosphorylation of the RNA polymerase II largest subunit during Xenopus laevis oocyte maturation.
Bellier S
,
Dubois MF
,
Nishida E
,
Almouzni G
,
Bensaude O
.
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Xenopus laevis oogenesis is characterized by an active transcription which ceases abruptly upon maturation. To survey changes in the characteristics of the transcriptional machinery which might contribute to this transcriptional arrest, the phosphorylation status of the RNA polymerase II largest subunit (RPB1 subunit) was analyzed during oocyte maturation. We found that the RPB1 subunit accumulates in large quantities from previtellogenic early diplotene oocytes up to fully grown oocytes. The C-terminal domain (CTD) of the RPB1 subunit was essentially hypophosphorylated in growing oocytes from Dumont stage IV to stage VI. Upon maturation, the proportion of hyperphosphorylated RPB1 subunits increased dramatically and abruptly. The hyperphosphorylated RPB1 subunits were dephosphorylated within 1 h after fertilization or heat shock of the matured oocytes. Extracts from metaphase II-arrested oocytes showed a much stronger CTD kinase activity than extracts from prophase stage VI oocytes. Most of this kinase activity was attributed to the activated Xp42 mitogen-activated protein (MAP) kinase, a MAP kinase of the ERK type. Making use of artificial maturation of the stage VI oocyte through microinjection of a recombinant stable cyclin B1, we observed a parallel activation of Xp42 MAP kinase and phosphorylation of RPB1. Both events required protein synthesis, which demonstrated that activation of p34(cdc2)off kinase was insufficient to phosphorylate RPB1 ex vivo and was consistent with a contribution of the Xp42 MAP kinase to RPB1 subunit phosphorylation. These results further support the possibility that the largest RNA polymerase II subunit is a substrate of the ERK-type MAP kinases during oocyte maturation, as previously proposed during stress or growth factor stimulation of mammalian cells.
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993, Pubmed,
Xenbase
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase
Almouzni,
Constraints on transcriptional activator function contribute to transcriptional quiescence during early Xenopus embryogenesis.
1995,
Pubmed
,
Xenbase
Baskaran,
Identification of a binding site in c-Ab1 tyrosine kinase for the C-terminal repeated domain of RNA polymerase II.
1996,
Pubmed
Brown,
Expression and activity of p40MO15, the catalytic subunit of cdk-activating kinase, during Xenopus oogenesis and embryogenesis.
1994,
Pubmed
,
Xenbase
Chambers,
The activity of COOH-terminal domain phosphatase is regulated by a docking site on RNA polymerase II and by the general transcription factors IIF and IIB.
1995,
Pubmed
Cisek,
Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2.
1989,
Pubmed
Cisek,
Purification of protein kinases that phosphorylate the repetitive carboxyl-terminal domain of eukaryotic RNA polymerase II.
1991,
Pubmed
Cismowski,
KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity.
1995,
Pubmed
Corden,
Tails of RNA polymerase II.
1990,
Pubmed
Corden,
RNA polymerase II transcription cycles.
1993,
Pubmed
Dahmus,
Phosphorylation of the C-terminal domain of RNA polymerase II.
1995,
Pubmed
Dahmus,
Reversible phosphorylation of the C-terminal domain of RNA polymerase II.
1996,
Pubmed
Dubois,
Inhibitors of transcription such as 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole and isoquinoline sulfonamide derivatives (H-8 and H-7) promote dephosphorylation of the carboxyl-terminal domain of RNA polymerase II largest subunit.
1994,
Pubmed
Dubois,
Enhanced phosphorylation of the C-terminal domain of RNA polymerase II upon serum stimulation of quiescent cells: possible involvement of MAP kinases.
1994,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dvir,
Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II.
1992,
Pubmed
Emili,
The RNA polymerase II carboxy-terminal domain: links to a bigger and better 'holoenzyme'?
1995,
Pubmed
Feaver,
Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.
1994,
Pubmed
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Haccard,
In vivo activation of a microtubule-associated protein kinase during meiotic maturation of the Xenopus oocyte.
1990,
Pubmed
,
Xenbase
Haccard,
Mitogen-activated protein kinase (MAP kinase) activation in Xenopus oocytes: roles of MPF and protein synthesis.
1993,
Pubmed
,
Xenbase
Hoeijmakers,
TFIIH: a key component in multiple DNA transactions.
1996,
Pubmed
Kim,
Immunochemical analysis of mammalian RNA polymerase II subspecies. Stability and relative in vivo concentration.
1986,
Pubmed
Koleske,
A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID.
1992,
Pubmed
Koleske,
The RNA polymerase II holoenzyme and its implications for gene regulation.
1995,
Pubmed
Krämer,
Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster.
1980,
Pubmed
LaMarca,
Hormonal effects on RNA synthesis by stage 6 oocytes of Xenopus laevis.
1975,
Pubmed
,
Xenbase
Lee,
CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae.
1991,
Pubmed
Liao,
A kinase-cyclin pair in the RNA polymerase II holoenzyme.
1995,
Pubmed
Maller,
Maturation-promoting factor in the early days.
1995,
Pubmed
,
Xenbase
Maxon,
Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance.
1994,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Nishida,
The MAP kinase cascade is essential for diverse signal transduction pathways.
1993,
Pubmed
,
Xenbase
Ossipow,
A mammalian RNA polymerase II holoenzyme containing all components required for promoter-specific transcription initiation.
1995,
Pubmed
Poon,
Cell cycle regulation of the p34cdc2/p33cdk2-activating kinase p40MO15.
1994,
Pubmed
,
Xenbase
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Rice,
RNA polymerase II is aberrantly phosphorylated and localized to viral replication compartments following herpes simplex virus infection.
1994,
Pubmed
Roeder,
Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Levels of activity during oocyte and embryonic development.
1974,
Pubmed
,
Xenbase
Roy,
The MO15 cell cycle kinase is associated with the TFIIH transcription-DNA repair factor.
1994,
Pubmed
Sawadogo,
RNA polymerase B (II) and general transcription factors.
1990,
Pubmed
Serizawa,
Association of Cdk-activating kinase subunits with transcription factor TFIIH.
1995,
Pubmed
Shiekhattar,
Cdk-activating kinase complex is a component of human transcription factor TFIIH.
1995,
Pubmed
Sterner,
The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex.
1995,
Pubmed
Svejstrup,
The multiple roles of transcription/repair factor TFIIH.
1996,
Pubmed
Tassan,
Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C.
1995,
Pubmed
Toyoda,
Characterization of RNA polymerase II-dependent transcription in Xenopus extracts.
1992,
Pubmed
,
Xenbase
Usheva,
Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein.
1992,
Pubmed
Valay,
Ccl1, a cyclin associated with protein kinase Kin28, controls the phosphorylation of RNA polymerase II largest subunit and mRNA transcription.
1996,
Pubmed
Valay,
The KIN28 gene is required both for RNA polymerase II mediated transcription and phosphorylation of the Rpb1p CTD.
1995,
Pubmed
Venetianer,
Phosphorylation state of the RNA polymerase II C-terminal domain (CTD) in heat-shocked cells. Possible involvement of the stress-activated mitogen-activated protein (MAP) kinases.
1995,
Pubmed
Yankulov,
The transcriptional elongation inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase.
1995,
Pubmed
