Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1997 Feb 18;944:1136-41. doi: 10.1073/pnas.94.4.1136.
Show Gene links
Show Anatomy links
Isoform-specific interactions of Na,K-ATPase subunits are mediated via extracellular domains and carbohydrates.
Schmalzing G
,
Ruhl K
,
Gloor SM
.
???displayArticle.abstract???
The functional unit of the Na,K-ATPase consists of a catalytic alpha subunit noncovalently linked with a glycoprotein subunit, beta. Using ouabain binding assays and immunoprecipitation of rodent alpha/beta complexes, we show here that all six possible isozymes between three alpha and two beta isoforms can be formed in Xenopus oocytes. Two isoform-specific differences in alpha/beta interactions are observed: (i) alpha1/beta1 and alpha2/beta2 complexes, in contrast to alpha1/beta2 complexes, are stable against Triton X-100-mediated dissociation, and (ii) beta2 subunits must carry N-glycans to combine with alpha1 but not with alpha2. The interacting surfaces are mainly exposed to the extracellular side because coexpression of a truncated beta1 subunit comprising the ectodomain results in assembly with alpha1 and alpha2, but not with alpha3; the beta2 ectodomain combines with alpha2 only. A chimera consisting of 81% and 19% of the alpha1 N terminus and alpha2 C terminus, respectively, behaves like alpha2 and coprecipitates with the beta2 ectodomain. In contrast, the reciprocal chimera does not coprecipitate with the beta2 ectodomain. These results provide evidence for a selective interaction of Na,K-ATPase alpha and beta subunits.
Anderberg,
Topological disposition of lysine 943 in native Na+/K(+)-transporting ATPase.
1995, Pubmed
Anderberg,
Topological disposition of lysine 943 in native Na+/K(+)-transporting ATPase.
1995,
Pubmed
Antolovic,
Epitope mapping by amino-acid-sequence-specific antibodies reveals that both ends of the alpha subunit of Na+/K(+)-ATPase are located on the cytoplasmic side of the membrane.
1991,
Pubmed
Bamberg,
cDNA cloning and membrane topology of the rabbit gastric H+/K(+)-ATPase alpha-subunit.
1992,
Pubmed
Beggah,
Hydrophobic C-terminal amino acids in the beta-subunit are involved in assembly with the alpha-subunit of Na,K-ATPase.
1993,
Pubmed
,
Xenbase
Blanco,
Comparison of the enzymatic properties of the Na,K-ATPase alpha 3 beta 1 and alpha 3 beta 2 isozymes.
1995,
Pubmed
Blanco,
Kinetic properties of the alpha 2 beta 1 and alpha 2 beta 2 isozymes of the Na,K-ATPase.
1995,
Pubmed
Cameron,
Neurons and astroglia express distinct subsets of Na,K-ATPase alpha and beta subunits.
1994,
Pubmed
Canfield,
Transmembrane organization of the Na,K-ATPase determined by epitope addition.
1993,
Pubmed
Chow,
Functional significance of the beta-subunit for heterodimeric P-type ATPases.
1995,
Pubmed
Eakle,
High-affinity ouabain binding by yeast cells expressing Na+, K(+)-ATPase alpha subunits and the gastric H+, K(+)-ATPase beta subunit.
1992,
Pubmed
Fambrough,
Analysis of subunit assembly of the Na-K-ATPase.
1994,
Pubmed
Felsenfeld,
Fine specificity mapping and topography of an isozyme-specific epitope of the Na,K-ATPase catalytic subunit.
1988,
Pubmed
Fisone,
Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K(+)-ATPase and effects of site-directed mutagenesis.
1994,
Pubmed
Geering,
A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Gloor,
Cloning and nucleotide sequence of the mouse Na,K-ATPase beta-subunit.
1989,
Pubmed
Gloor,
The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase.
1990,
Pubmed
Gloor,
Production and secretion in CHO cells of the extracellular domain of AMOG/beta 2, a type-II membrane protein.
1992,
Pubmed
Gloor,
A vector for the synthesis of cRNAs encoding Myc epitope-tagged proteins in Xenopus laevis oocytes.
1995,
Pubmed
,
Xenbase
Goldshleger,
Topology of the alpha-subunit of Na,K-ATPase based on proteolysis. Lability of the topological organization.
1995,
Pubmed
Gottardi,
Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly.
1993,
Pubmed
Hamrick,
Assembly of the extracellular domain of the Na,K-ATPase beta subunit with the alpha subunit. Analysis of beta subunit chimeras and carboxyl-terminal deletions.
1993,
Pubmed
Herrera,
Developmental cell-specific regulation of Na(+)-K(+)-ATPase alpha 1-, alpha 2-, and alpha 3-isoform gene expression.
1994,
Pubmed
Herrera,
Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications.
1987,
Pubmed
Horisberger,
The H,K-ATPase beta-subunit can act as a surrogate for the beta-subunit of Na,K-pumps.
1991,
Pubmed
,
Xenbase
Jaunin,
Role of the transmembrane and extracytoplasmic domain of beta subunits in subunit assembly, intracellular transport, and functional expression of Na,K-pumps.
1993,
Pubmed
,
Xenbase
Karlish,
Location of Asn831 of the alpha chain of Na/K-ATPase at the cytoplasmic surface. Implication for topological models.
1993,
Pubmed
Lemas,
26 amino acids of an extracellular domain of the Na,K-ATPase alpha-subunit are sufficient for assembly with the Na,K-ATPase beta-subunit.
1994,
Pubmed
Lingrel,
Molecular genetics of Na,K-ATPase.
1990,
Pubmed
Lingrel,
Na,K-ATPase: isoform structure, function, and expression.
1992,
Pubmed
Mays,
Hierarchy of mechanisms involved in generating Na/K-ATPase polarity in MDCK epithelial cells.
1995,
Pubmed
McDonough,
Physiologic rationale for multiple sodium pump isoforms. Differential regulation of alpha 1 vs alpha 2 by ionic stimuli.
1992,
Pubmed
McGrail,
Immunofluorescent localization of three Na,K-ATPase isozymes in the rat central nervous system: both neurons and glia can express more than one Na,K-ATPase.
1991,
Pubmed
Noguchi,
Expression of functional (Na+ + K+)-ATPase from cloned cDNAs.
1987,
Pubmed
,
Xenbase
Noguchi,
Assembly of a hybrid from the alpha subunit of Na+/K(+)-ATPase and the beta subunit of H+/K(+)-ATPase.
1992,
Pubmed
,
Xenbase
Orlowski,
Tissue-specific and developmental regulation of rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs.
1988,
Pubmed
Pietrini,
Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.
1992,
Pubmed
Renaud,
Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
1991,
Pubmed
Schmalzing,
Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Schmalzing,
Up-regulation of sodium pump activity in Xenopus laevis oocytes by expression of heterologous beta 1 subunits of the sodium pump.
1991,
Pubmed
,
Xenbase
Schmalzing,
The adhesion molecule on glia (AMOG/beta 2) and alpha 1 subunits assemble to functional sodium pumps in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Shull,
Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.
1986,
Pubmed
Sweadner,
Isozymes of the Na+/K+-ATPase.
1989,
Pubmed
Sánchez del Pino,
Biochemical discrimination between luminal and abluminal enzyme and transport activities of the blood-brain barrier.
1995,
Pubmed
Takeyasu,
Stability of Na(+)-K(+)-ATPase alpha-subunit isoforms in evolution.
1990,
Pubmed
Vasilets,
Structure-function relationships of cation binding in the Na+/K(+)-ATPase.
1993,
Pubmed
Watts,
Cell-specific expression of mRNAs encoding Na+,K(+)-ATPase alpha- and beta-subunit isoforms within the rat central nervous system.
1991,
Pubmed
Zlokovic,
Differential expression of Na,K-ATPase alpha and beta subunit isoforms at the blood-brain barrier and the choroid plexus.
1993,
Pubmed
