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Mech Dev January 1, 1997; 61 (1-2): 127-40.

The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation.

Meersseman G , Verschueren K , Nelles L , Blumenstock C , Kraft H , Wuytens G , Remacle J , Kozak CA , Tylzanowski P , Niehrs C , Huylebroeck D .

We report the characterization of two vertebrate homologs of Drosophila mothers against dpp (Mad) isolated from the mouse and the Xenopus embryo, named MusMLP (mad-like protein) and XenMLP, respectively, together with a summary of their expression patterns in the embryo. Overexpression of XenMLP causes ventralization of Xenopus embryos and we demonstrate that the C-terminal domain is necessary and sufficient to confer this biological effect. This domain also has the potential for transcriptional activation, as shown in one-hybrid assays in mammalian cells. We further demonstrate that MLPs are multidomain proteins by showing a cis-negative effect of the N-terminal domain on the transactivation by the C-terminal domain and that the proline-rich, middle domain maximizes the activity of the C-terminal domain. We also mapped the MusMLP gene to a region on mouse chromosome 13 that corresponds to a region on human chromosome 5q that contains cancer-related genes.

PubMed ID: 9076683
Article link:

Species referenced: Xenopus
Genes referenced: csrp3 dspp gnao1 h4c4 smad1 smad3 tbx2 ventx1.2

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