Dev Biol 1996 Nov 01;1792:485-92. doi: 10.1006/dbio.1996.0277.

Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.

???displayArticle.abstract???
Mos is a serine-threonine protein kinase and a key regulator of meiosis. One function of Xenopus Mos is to activate mitogen-activated protein kinase (MAPK) through direct phosphorylation and activation of MAPK kinase (MAPKK). All three members of this signal cascade can individually induce hormone-independent reentry of oocytes into meiosis I. However, their inducing efficiency is reduced in the absence of protein synthesis. Here we show that de novo Mos synthesis is required for induction of meiosis I by active MAPKK or Mos-MAPK coinjection. In addition, MAPK efficiently phosphorylates Mos at Ser-3 in vitro. These results suggest that a positive feedback loop exists between MAPK and Mos during oocyte maturation. De novo synthesis of Mos, and other proteins, is required for progression from meiosis I to the metaphase arrest at meiosis II; therefore, one function of MAPK during normal Xenopus oocyte maturation might be to stimulate the synthesis or accumulation of Mos that is required for the completion of meiosis.

???displayArticle.pubmedLink??? 8903362
???displayArticle.link??? Dev Biol
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: mapk1 mos