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XB-ART-17524
Cell November 1, 1996; 87 (3): 447-58.
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A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.

Merdes A , Ramyar K , Vechio JD , Cleveland DW .


Abstract
NuMA is a nuclear protein during interphase but redistributes to the spindle poles early in mitosis. To investigate its role during spindle formation, we tested spindle assembly in frog egg extracts from which NuMA was immunodepleted. Immunodepletion revealed that NuMA forms a complex with cytoplasmic dynein and dynactin. The depleted extracts failed to assemble normal mitotic spindles, producing, instead, chromatin-associated irregular arrays of microtubules lacking characteristic spindle poles. A subdomain of the NuMA tail was shown to induce microtubule aster formation by mediating microtubule bundling. Our findings suggest that NuMA forms bifunctional complexes with cytoplasmic dynein and dynactin that can tether microtubules at the spindle poles and that are essential for mitotic spindle pole assembly and stabilization.

PubMed ID: 8898198
Article link: Cell
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dnai1 dync1li1 numa1
Antibodies: Numa1 Ab1