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XB-ART-17702
Arch Biochem Biophys September 15, 1996; 333 (2): 326-32.
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The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.

Quill TA , Hedrick JL .


Abstract
The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CGL) ligand which can detect the presence of the CGL ligand at a sensitivity of 1-2 ng/ml. This assay is specifically inhibited with galactose, 50% inhibition at 9.9 mM, and produces a linear response between 2 and 20 ng of jelly adsorbed to the microtiter plate. Using this assay, the CGL ligand was purified through gel filtration, anion-exchange, and CGL affinity chromatography. Hydrolysis of the purified CGL ligand with a series of exoglycosidases showed that a terminal alpha-galactose is the ligand structure required for recognition by CGL.

PubMed ID: 8809070
Article link: Arch Biochem Biophys
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cgl.2