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Regulation of embryonic cell division by a Xenopus gastrula-specific protein kinase.
Snape AM
,
Smith JC
.
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We describe a novel protein kinase, Pk9.7, and its role in cell division in the Xenopus embryo. Pk9.7 is transcribed only during blastula and gastrula stages. Expression of Pk9.7 in Xenopus oocytes induces meiotic maturation, while overexpression in embryos blocks blastomerecleavage in a MAP kinase-independent fashion. In both Pk9.7-injected oocytes and mitotic cells of cleavage-blocked embryos, chromosomes appear detached from abnormal spindles, and in oocytes additional microtubule structures are formed, suggesting that one function of Pk9.7 is to regulate formation of, and chromosome attachment to, the spindle. Consistent with this, Pk9.7 co-immunoprecipitates tubulin and phosphorylates it in vitro. Pk9.7 expression coincides with the switch from maternal to zygotic control of the cell cycle, and with the switch from microtubule independence to microtubule dependence. Our results suggest that Pk9.7 plays a role in these processes.
Almouzni,
Constraints on transcriptional activator function contribute to transcriptional quiescence during early Xenopus embryogenesis.
1995, Pubmed,
Xenbase
Almouzni,
Constraints on transcriptional activator function contribute to transcriptional quiescence during early Xenopus embryogenesis.
1995,
Pubmed
,
Xenbase
Campbell,
Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase.
1995,
Pubmed
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Clute,
Regulation of the appearance of division asynchrony and microtubule-dependent chromosome cycles in Xenopus laevis embryos.
1995,
Pubmed
,
Xenbase
Cunliffe,
Specification of mesodermal pattern in Xenopus laevis by interactions between Brachyury, noggin and Xwnt-8.
1994,
Pubmed
,
Xenbase
Daar,
The ras oncoprotein and M-phase activity.
1991,
Pubmed
,
Xenbase
Daar,
Inhibition of mos-induced oocyte maturation by protein kinase A.
1993,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Edgar,
Transcriptional regulation of string (cdc25): a link between developmental programming and the cell cycle.
1994,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed
Gotoh,
Involvement of the MAP kinase cascade in Xenopus mesoderm induction.
1995,
Pubmed
,
Xenbase
Gotoh,
The MAP kinase cascade: its role in Xenopus oocytes, eggs and embryos.
1995,
Pubmed
,
Xenbase
Graves,
Fibroblast growth factor, but not activin, is a potent activator of mitogen-activated protein kinase in Xenopus explants.
1994,
Pubmed
,
Xenbase
Green,
Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm.
1992,
Pubmed
,
Xenbase
Gurdon,
Transcription of muscle-specific actin genes in early Xenopus development: nuclear transplantation and cell dissociation.
1984,
Pubmed
,
Xenbase
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Houliston,
Patterns of microtubule polymerization relating to cortical rotation in Xenopus laevis eggs.
1991,
Pubmed
,
Xenbase
Howe,
Identification of a developmental timer regulating the stability of embryonic cyclin A and a new somatic A-type cyclin at gastrulation.
1995,
Pubmed
,
Xenbase
Isaacs,
Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification.
1992,
Pubmed
,
Xenbase
King,
Mitosis in transition.
1994,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
LaBonne,
Role of MAP kinase in mesoderm induction and axial patterning during Xenopus development.
1995,
Pubmed
,
Xenbase
MacNicol,
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
1993,
Pubmed
,
Xenbase
MacNicol,
Regulation of Raf-1-dependent signaling during early Xenopus development.
1995,
Pubmed
,
Xenbase
Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase
Meyerson,
A family of human cdc2-related protein kinases.
1992,
Pubmed
Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Mohun,
A third striated muscle actin gene is expressed during early development in the amphibian Xenopus laevis.
1988,
Pubmed
,
Xenbase
Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase
Nebreda,
p21ras-induced meiotic maturation of Xenopus oocytes in the absence of protein synthesis: MPF activation is preceded by activation of MAP and S6 kinases.
1993,
Pubmed
,
Xenbase
Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Nicklas,
Odd chromosome movement and inaccurate chromosome distribution in mitosis and meiosis after treatment with protein kinase inhibitors.
1993,
Pubmed
Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase
Rupp,
Xenopus embryos regulate the nuclear localization of XMyoD.
1994,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Sargent,
Identification in Xenopus of a structural homologue of the Drosophila gene snail.
1990,
Pubmed
,
Xenbase
Scavo,
Genes encoding receptors for insulin and insulin-like growth factor I are expressed in Xenopus oocytes and embryos.
1991,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Steele,
The yes proto-oncogene is present in amphibians and contributes to the maternal RNA pool in the oocyte.
1989,
Pubmed
,
Xenbase
Thomas,
Cell cycle progression in the developing Drosophila eye: roughex encodes a novel protein required for the establishment of G1.
1994,
Pubmed
Toda,
Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.
1987,
Pubmed
Umbhauer,
Mesoderm induction in Xenopus caused by activation of MAP kinase.
1995,
Pubmed
,
Xenbase
Winning,
Pagliaccio, a member of the Eph family of receptor tyrosine kinase genes, has localized expression in a subset of neural crest and neural tissues in Xenopus laevis embryos.
1994,
Pubmed
,
Xenbase
Yasuda,
Temporal regulation in the early embryo: is MBT too good to be true?
1992,
Pubmed
,
Xenbase
Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
Xenbase
Yi,
Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.
1991,
Pubmed
Zhou,
Ability of the c-mos product to associate with and phosphorylate tubulin.
1991,
Pubmed
,
Xenbase