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EMBO J
2005 Jun 15;2412:2172-82. doi: 10.1038/sj.emboj.7600700.
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PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.
Azuma Y
,
Arnaoutov A
,
Anan T
,
Dasso M
.
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Here we show that the PIASy protein is specifically required for mitotic modification of Topoisomerase-II by SUMO-2 conjugation in Xenopus egg extracts. PIASy was unique among the PIAS family members in its capacity to bind mitotic chromosomes and recruit Ubc9 onto chromatin. These properties were essential, since PIASy mutants that did not bind chromatin or failed to recruit Ubc9 were functionally inactive. We observed that PIASy depletion eliminated essentially all chromosomal accumulation of EGFP-SUMO-2-conjugated species, suggesting that it is the primary E3-like factor for mitotic chromosomal substrates of SUMO-2. PIASy-dependent SUMO-2-conjugated species were highly concentrated on the inner centromere, and inhibition of PIASy blocked anaphase sister chromatid segregation in egg extracts. Taken together, our observations suggest that PIASy is a critical regulator of mitotic SUMO-2 conjugation for Topoisomerase-II and other chromosomal substrates, and that its activity may have particular relevance for centromeric functions required for proper chromosome segregation.
Aravind,
SAP - a putative DNA-binding motif involved in chromosomal organization.
2000, Pubmed
Aravind,
SAP - a putative DNA-binding motif involved in chromosomal organization.
2000,
Pubmed
Arnaoutov,
The Ran GTPase regulates kinetochore function.
2003,
Pubmed
,
Xenbase
Ayaydin,
Distinct in vivo dynamics of vertebrate SUMO paralogues.
2004,
Pubmed
Azuma,
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
2001,
Pubmed
Azuma,
SUMO-2/3 regulates topoisomerase II in mitosis.
2003,
Pubmed
,
Xenbase
Bachant,
The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II.
2002,
Pubmed
Bernier-Villamor,
Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.
2002,
Pubmed
Chun,
Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy.
2003,
Pubmed
Cleveland,
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
2003,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Hari,
The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family.
2001,
Pubmed
Hauf,
Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.
2001,
Pubmed
Hochstrasser,
SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
2001,
Pubmed
Johnson,
Protein modification by SUMO.
2004,
Pubmed
Luke,
Quantitation of type II topoisomerase in oocytes and eggs of Xenopus laevis.
1989,
Pubmed
,
Xenbase
McCleland,
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity.
2003,
Pubmed
,
Xenbase
Melchior,
SUMO: ligases, isopeptidases and nuclear pores.
2003,
Pubmed
Minty,
Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif.
2000,
Pubmed
Okubo,
NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
2004,
Pubmed
Sachdev,
PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies.
2001,
Pubmed
Wong,
Protein inhibitor of activated STAT Y (PIASy) and a splice variant lacking exon 6 enhance sumoylation but are not essential for embryogenesis and adult life.
2004,
Pubmed
Wustmann,
The genetics of position-effect variegation modifying loci in Drosophila melanogaster.
1989,
Pubmed
Xhemalce,
Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance.
2004,
Pubmed
Zhao,
A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization.
2005,
Pubmed
