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Proc Natl Acad Sci U S A
1996 May 28;9311:5347-51. doi: 10.1073/pnas.93.11.5347.
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Expression of a renal type I sodium/phosphate transporter (NaPi-1) induces a conductance in Xenopus oocytes permeable for organic and inorganic anions.
Busch AE
,
Schuster A
,
Waldegger S
,
Wagner CA
,
Zempel G
,
Broer S
,
Biber J
,
Murer H
,
Lang F
.
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Two distinct molecular types (I and II) of renal proximal tubular brush border Na+/Pi cotransporters have been identified by expression cloning on the basis of their capacity to induce Na+-dependent Pi influx in tracer experiments. Whereas the type II transporters (e.g., NaPi-2 and NaPi-3) resemble well known characteristics of brush border Na+/Pi cotransport, little is known about the properties of the type I transporter (NaPi-1). In contrast to type II, type I transporters produced electrogenic transport only at high extracellular Pi concentrations (> or =3 mM). On the other hand, expression of NaPi-1 induced a Cl- conductance in Xenopus laevis oocytes, which was inhibited by Cl- channel blockers [5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) > niflumic acid > 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid]. Further, the Cl- conductance was inhibited by the organic anions phenol red, benzylpenicillin (penicillin G), and probenecid. These organic anions induced outwardly directed currents in the absence of Cl-. In tracer studies, we observed uptake of benzylpenicillin with a Km of 0.22 mM; benzylpenicillin uptake was inhibited by NPPB and niflumic acid. These findings suggest that the type I Na+/Pi cotransporter functions also as a novel type of anion channel permeable not only for Cl- but also for organic anions. Such an apical anion channel could serve an important role in the transport of Cl- and the excretion of anionic xenobiotics.
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Bertran,
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,
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Biber,
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Bröer,
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,
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Busch,
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,
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Busch,
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,
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Busch,
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,
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Chong,
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Custer,
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,
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Hartmann,
Transport characteristics of a murine renal Na/Pi-cotransporter.
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,
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Jentsch,
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Johnson,
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Levi,
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Levi,
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Magagnin,
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,
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Murer,
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Ni,
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,
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Paulmichl,
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,
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Tenenhouse,
Renal Na(+)-phosphate cotransport in murine X-linked hypophosphatemic rickets. Molecular characterization.
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,
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Tenenhouse,
Effect of P(i) restriction on renal Na(+)-P(i) cotransporter mRNA and immunoreactive protein in X-linked Hyp mice.
1995,
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Verri,
Cloning of a rabbit renal Na-Pi cotransporter, which is regulated by dietary phosphate.
1995,
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,
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Welsh,
Cystic fibrosis transmembrane conductance regulator: a chloride channel with novel regulation.
1992,
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Werner,
Increase of Na/Pi-cotransport encoding mRNA in response to low Pi diet in rat kidney cortex.
1994,
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,
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Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
