Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases.
Martin G
,
Keller W
.
???displayArticle.abstract???
We have tested deletion and substitution mutants of bovine poly(A) polymerase, and have identified a small region that overlaps with a nuclear localization signal and binds to the RNA primer. Systematic mutagenesis of carboxylic amino acids led to the identification of three aspartates that are essential for catalysis. Sequence and secondary structure comparisons of regions surrounding these aspartates with sequences of other polymerases revealed a significant homology to the palm structure of DNA polymerase beta, terminal deoxynucleotidyltransferase and DNA polymerase IV of Saccharomyces cerevisiae, all members of the family X of polymerases. This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor.
Aebi,
Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.
1990, Pubmed
Aebi,
Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.
1990,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Argos,
A sequence motif in many polymerases.
1988,
Pubmed
Astatke,
Deoxynucleoside triphosphate and pyrophosphate binding sites in the catalytically competent ternary complex for the polymerase reaction catalyzed by DNA polymerase I (Klenow fragment).
1995,
Pubmed
Bairoch,
The SWISS-PROT protein sequence data bank, recent developments.
1993,
Pubmed
Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase
Beese,
Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate.
1993,
Pubmed
Bienroth,
Assembly of a processive messenger RNA polyadenylation complex.
1993,
Pubmed
Bienroth,
Purification of the cleavage and polyadenylation factor involved in the 3'-processing of messenger RNA precursors.
1991,
Pubmed
Birney,
Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.
1993,
Pubmed
Bonner,
Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure.
1992,
Pubmed
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Carey,
Gel retardation.
1991,
Pubmed
Christofori,
3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP.
1988,
Pubmed
Clos,
Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation.
1990,
Pubmed
,
Xenbase
Cudny,
Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase.
1986,
Pubmed
Date,
Aspartic acid residues at positions 190 and 192 of rat DNA polymerase beta are involved in primer binding.
1991,
Pubmed
Davies,
2.3 A crystal structure of the catalytic domain of DNA polymerase beta.
1994,
Pubmed
Delarue,
An attempt to unify the structure of polymerases.
1990,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Dingwall,
Nuclear targeting sequences--a consensus?
1991,
Pubmed
Dong,
Mutational studies of human DNA polymerase alpha. Lysine 950 in the third most conserved region of alpha-like DNA polymerases is involved in binding the deoxynucleoside triphosphate.
1995,
Pubmed
Gibson,
The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid.
1993,
Pubmed
Gibson,
KH domains within the FMR1 sequence suggest that fragile X syndrome stems from a defect in RNA metabolism.
1993,
Pubmed
Gribskov,
Profile analysis: detection of distantly related proteins.
1987,
Pubmed
Gunderson,
The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase.
1994,
Pubmed
Hager,
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
1980,
Pubmed
Haynes,
The RNP motif protein family.
1992,
Pubmed
Higgins,
The EMBL Data Library.
1992,
Pubmed
Holm,
DNA polymerase beta belongs to an ancient nucleotidyltransferase superfamily.
1995,
Pubmed
Ito,
Compilation and alignment of DNA polymerase sequences.
1991,
Pubmed
Joyce,
Function and structure relationships in DNA polymerases.
1994,
Pubmed
Keller,
No end yet to messenger RNA 3' processing!
1995,
Pubmed
Keller,
Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA.
1991,
Pubmed
Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed
Kim,
Crystal structure of Thermus aquaticus DNA polymerase.
1995,
Pubmed
Kohlstaedt,
Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor.
1992,
Pubmed
Korolev,
Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability.
1995,
Pubmed
LaCasse,
Nuclear localization signals overlap DNA- or RNA-binding domains in nucleic acid-binding proteins.
1995,
Pubmed
Lingner,
Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae.
1991,
Pubmed
Manley,
A complex protein assembly catalyzes polyadenylation of mRNA precursors.
1995,
Pubmed
Mikaelian,
A general and fast method to generate multiple site directed mutations.
1992,
Pubmed
Nagai,
The RNP domain: a sequence-specific RNA-binding domain involved in processing and transport of RNA.
1995,
Pubmed
Ollis,
Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase.
,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Pedersen,
Structural investigation of the antibiotic and ATP-binding sites in kanamycin nucleotidyltransferase.
1995,
Pubmed
Pelletier,
Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP.
1994,
Pubmed
Poch,
Identification of four conserved motifs among the RNA-dependent polymerase encoding elements.
1989,
Pubmed
Polesky,
Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.
1990,
Pubmed
Prasad,
Yeast open reading frame YCR14C encodes a DNA beta-polymerase-like enzyme.
1993,
Pubmed
Raabe,
Poly(A) polymerase contains multiple functional domains.
1994,
Pubmed
Raabe,
Primary structure and expression of bovine poly(A) polymerase.
1991,
Pubmed
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Rost,
Prediction of protein secondary structure at better than 70% accuracy.
1993,
Pubmed
Rost,
Combining evolutionary information and neural networks to predict protein secondary structure.
1994,
Pubmed
Sakon,
Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution.
1993,
Pubmed
Sawaya,
Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism.
1994,
Pubmed
Shaw,
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
1993,
Pubmed
Sousa,
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution.
1993,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Thuresson,
Multiple forms of poly(A) polymerases in human cells.
1994,
Pubmed
Wahle,
Purification and characterization of a mammalian polyadenylate polymerase involved in the 3' end processing of messenger RNA precursors.
1991,
Pubmed
Wahle,
A novel poly(A)-binding protein acts as a specificity factor in the second phase of messenger RNA polyadenylation.
1991,
Pubmed
Wahle,
Mammalian poly(A)-binding protein II. Physical properties and binding to polynucleotides.
1993,
Pubmed
Wahle,
3'-end cleavage and polyadenylation of mRNA precursors.
1995,
Pubmed
Wahle,
Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase.
1991,
Pubmed
Wilson,
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
1994,
Pubmed
Yang,
Recombining the structures of HIV integrase, RuvC and RNase H.
1995,
Pubmed
Zhelkovsky,
Structure-function relationships in the Saccharomyces cerevisiae poly(A) polymerase. Identification of a novel RNA binding site and a domain that interacts with specificity factor(s).
1995,
Pubmed
,
Xenbase
