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Proc Natl Acad Sci U S A
1995 Aug 29;9218:8298-302.
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Toward the therapeutic editing of mutated RNA sequences.
Woolf TM
,
Chase JM
,
Stinchcomb DT
.
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If RNA editing could be rationally directed to mutated RNA sequences, genetic diseases caused by certain base substitutions could be treated. Here we use a synthetic complementary RNA oligonucleotide to direct the correction of a premature stop codon mutation in dystrophin RNA. The complementary RNA oligonucleotide was hybridized to a premature stop codon and the hybrid was treated with nuclear extracts containing the cellular enzyme double-stranded RNA adenosine deaminase. When the treated RNAs were translated in vitro, a dramatic increase in expression of a downstream luciferase coding region was observed. The cDNA sequence data are consistent with deamination of the adenosine in the UAG stop codon to inosine by double-stranded RNA adenosine deaminase. Injection of oligonucleotide-mRNA hybrids into Xenopus embryos also resulted in an increase in luciferase expression. These experiments demonstrate the principle of therapeutic RNA editing.
Backus,
Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.
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Backus,
Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.
1994,
Pubmed
Bartel,
Isolation of new ribozymes from a large pool of random sequences [see comment].
1993,
Pubmed
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase
Blum,
A model for RNA editing in kinetoplastid mitochondria: "guide" RNA molecules transcribed from maxicircle DNA provide the edited information.
1990,
Pubmed
Bulman,
Point mutation in the human dystrophin gene: identification through western blot analysis.
1991,
Pubmed
Dai,
Cleavage of an amide bond by a ribozyme.
1995,
Pubmed
Dominski,
Restoration of correct splicing in thalassemic pre-mRNA by antisense oligonucleotides.
1993,
Pubmed
Higuchi,
RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency.
1993,
Pubmed
Lamond,
2'-O-alkyloligoribonucleotides: probes for studying the biochemistry and cell biology of RNA processing.
1993,
Pubmed
Maher,
Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylphosphonates in a cell-free system.
1988,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Monia,
Selective inhibition of mutant Ha-ras mRNA expression by antisense oligonucleotides.
1992,
Pubmed
Navaratnam,
The p27 catalytic subunit of the apolipoprotein B mRNA editing enzyme is a cytidine deaminase.
1993,
Pubmed
,
Xenbase
Polson,
Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase.
1994,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Roberts,
Searching for the 1 in 2,400,000: a review of dystrophin gene point mutations.
1994,
Pubmed
Saccomanno,
The cytoplasm of Xenopus oocytes contains a factor that protects double-stranded RNA from adenosine-to-inosine modification.
1994,
Pubmed
,
Xenbase
Scaringe,
Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites.
1990,
Pubmed
Simpson,
RNA editing--a novel genetic phenomenon?
1990,
Pubmed
Sullenger,
Ribozyme-mediated repair of defective mRNA by targeted, trans-splicing.
1994,
Pubmed
Wagner,
A double-stranded RNA unwinding activity introduces structural alterations by means of adenosine to inosine conversions in mammalian cells and Xenopus eggs.
1989,
Pubmed
,
Xenbase
