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Nature June 1, 1995; 375 (6530): 421-4.

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.

Madine MA , Khoo CY , Mills AD , Laskey RA .

An intact nuclear membrane restricts DNA replication to only one round in each cell cycle, apparently by excluding an essential replication-licensing factor throughout interphase. A family of related yeast replication proteins, MCM2, 3 and 5 (also called, after cell-division cycle, CDC46), resemble licensing factor, entering the nucleus only during mitosis. We have cloned a Xenopus homologue of MCM3 (XMCM3) and raised antibodies against expressed protein. Immunodepletion of Xenopus egg extracts removes a complex of MCM2, 3 and 5 homologues and inhibits replication of Xenopus sperm nuclei or permeable G2 HeLa nuclei. However, G1 HeLa nuclei still replicate efficiently. Mock-depleted extracts replicate all three templates. XMCM3 accumulates in nuclei before replication but anti-XMCM3 staining decreases during replication. These results can explain why replicated nuclei are unable to reinitiate replication in a single cell cycle.

PubMed ID: 7760938
Article link: Nature
Grant support: [+]

Species referenced: Xenopus
Genes referenced: mcm2 mcm3 mcm5

References :
Huberman, Cell cycle. A licence to replicate. 1995, Pubmed, Xenbase