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XB-ART-19666
Development June 1, 1995; 121 (6): 1927-35.
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Dorsal-ventral patterning and differentiation of noggin-induced neural tissue in the absence of mesoderm.

Knecht AK , Good PJ , Dawid IB , Harland RM .


Abstract
In Xenopus development, dorsal mesoderm is thought to play a key role in both induction and patterning of the nervous system. Previously, we identified a secreted factor, noggin, which is expressed in dorsal mesoderm and which can mimic that tissue''s neural-inducing activity, without inducing mesoderm. Here the neural tissue induced in ectodermal explants by noggin is further characterized using four neural-specific genes: two putative RNA-binding proteins, nrp-1 and etr-1; the synaptobrevin sybII; and the lipocalin cpl-1. First we determine the expression domain of each gene during embryogenesis. Then we analyze expression of these genes in noggin-treated explants. All markers, including the differentiated marker sybII, are expressed in noggin-induced neural tissue. Furthermore, cpl-1, a marker of dorsal brain, and etr-1, a marker absent in much of the dorsal forebrain, are expressed in non-overlapping territories within these explants. We conclude that the despite the absence of mesoderm, noggin-induced neural tissue shows considerable differentiation and organization, which may represent dorsal-ventral patterning of the forebrain.

PubMed ID: 7601005
Article link:


Species referenced: Xenopus
Genes referenced: ag1 celf3 ctsl nog nrp1 ptgds tbx2 vamp1 vamp2


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