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Mol Cell Biol
1995 Mar 01;153:1422-30. doi: 10.1128/MCB.15.3.1422.
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Cloning and characterization of a Xenopus poly(A) polymerase.
Gebauer F
,
Richter JD
.
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During oocyte maturation and early embryogenesis in Xenopus laevis, the translation of several mRNAs is regulated by cytoplasmic poly(A) elongation, a reaction catalyzed by poly(A) polymerase (PAP). We have cloned, sequenced, and examined several biochemical properties of a Xenopus PAP. This protein is 87% identical to the amino-terminal portion of bovine PAP, which catalyzes the nuclear polyadenylation reaction, but lacks a large region of the corresponding carboxy terminus, which contains the nuclear localization signal. When injected into oocytes, the Xenopus PAP remains concentrated in the cytoplasm, suggesting that it is a specifically cytoplasmic enzyme. Oocytes contain several PAP mRNA-related transcripts, and the levels of at least the one encoding the putative cytoplasmic enzyme are relatively constant in oocytes and early embryos but decline after blastulation. When expressed in bacteria and purified by affinity and MonoQ-Sepharose chromatography, the protein has enzymatic activity and adds poly(A) to a model substrate. Importantly, affinity-purified antibodies directed against Xenopus PAP inhibit cytoplasmic polyadenylation in egg extracts. These data suggest that the PAP described here could participate in cytoplasmic polyadenylation during Xenopus oocyte maturation.
Bilger,
Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements.
1994,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cao,
Identification of the gene for an Escherichia coli poly(A) polymerase.
1992,
Pubmed
Dworkin,
Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Edmonds,
Polyadenylate polymerases.
1990,
Pubmed
Egrie,
Changes in poly(adenylic acid) polymerase activity during sea urchin embryogenesis.
1979,
Pubmed
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Fox,
Polyadenylation of maternal mRNA during oocyte maturation: poly(A) addition in vitro requires a regulated RNA binding activity and a poly(A) polymerase.
1992,
Pubmed
Gebauer,
Cloning and characterization of a Xenopus poly(A) polymerase.
1995,
Pubmed
,
Xenbase
Gershon,
Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus.
1991,
Pubmed
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Huarte,
Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
1987,
Pubmed
Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase
Kormanec,
Simple and reliable determination of radioactive precursors incorporation into nucleic acids in vitro.
1993,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lingner,
Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae.
1991,
Pubmed
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Niessing,
Evidence for the existence of two different poly(A) polymerases and a novel ribohomopolymer polymerase in rat liver nuclei.
1974,
Pubmed
Olmsted,
Analysis of cytoskeletal structures using blot-purified monospecific antibodies.
1986,
Pubmed
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Paris,
Maturation-specific polyadenylation and translational control: diversity of cytoplasmic polyadenylation elements, influence of poly(A) tail size, and formation of stable polyadenylation complexes.
1990,
Pubmed
,
Xenbase
Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase
Pellicer,
Characterization of two poly(A) polymerases from cultured hamster fibroblasts.
1978,
Pubmed
Raabe,
Primary structure and expression of bovine poly(A) polymerase.
1991,
Pubmed
Raabe,
Poly(A) polymerase contains multiple functional domains.
1994,
Pubmed
Richter,
Translational control in development: a perspective.
1993,
Pubmed
,
Xenbase
Rosenthal,
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
1983,
Pubmed
Ryner,
Multiple forms of poly(A) polymerases purified from HeLa cells function in specific mRNA 3'-end formation.
1989,
Pubmed
Sachs,
Poly(A) tail metabolism and function in eucaryotes.
1993,
Pubmed
Sallés,
Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation.
1992,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Simon,
Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element.
1992,
Pubmed
,
Xenbase
Simon,
Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins.
1994,
Pubmed
,
Xenbase
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Stetler,
Structurally and immunologically distinct poly(A) polymerases in rat liver. Occurrence of a tumor-type enzyme in normal liver.
1984,
Pubmed
Stetler,
Comparison of cytosolic and nuclear poly(A) polymerases from rat liver and a hepatoma: structural and immunological properties and response to NI-type protein kinases.
1985,
Pubmed
Thuresson,
Multiple forms of poly(A) polymerases in human cells.
1994,
Pubmed
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed
Wahle,
Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase.
1991,
Pubmed
Wahle,
The end of the message: 3'-end processing leading to polyadenylated messenger RNA.
1992,
Pubmed
Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed
Wormington,
Poly(A) and translation: development control.
1993,
Pubmed
,
Xenbase
