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Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression.
Miyamoto K
,
Tatsumi S
,
Sonoda T
,
Yamamoto H
,
Minami H
,
Taketani Y
,
Takeda E
.
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A cDNA clone encoding a protein 69% identical in amino acid sequence with that of the Na/P(i) co-transporter NaP(i)-1 was isolated from a human kidney cDNA library. The DNA sequence was identical with that of NPT-1 cDNA published by Chong, Kristjansson, Zoghbi and Hughe (1993) (Genomics, 18, 355-359). In the present study, we have characterized the function of the encoded protein and the tissue distribution of its mRNA. Injection of RNA transcribed from NPT-1 into Xenopus oocytes resulted in expression of Na/P(i) co-transport activity showing a high affinity for P(i) transport (Km 0.29 mM). Kinetic characterization ([P(i)], [Na+]) demonstrated that the expressed transport activity has properties similar to those displayed by oocytes injected with human kidney poly(A)+ RNA. Northern blotting demonstrated that NPT-1 mRNA is expressed in renal cortex, liver and brain but not in other tissues. Hybrid depletion with antisense oligonucleotides to NaP(i)-3 and NPT-1 completely inhibited poly(A)+ RNA-induced Na(+)-dependent P(i) uptake in oocytes. These findings indicate that two high-affinity Na/P(i) cotransporters (NaP(i)-3 and NPT-1) are present in human kidney cortex.
Biber,
Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.
1993, Pubmed
Biber,
Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.
1993,
Pubmed
Bindels,
Effect of pH on the kinetics of Na+-dependent phosphate transport in rat renal brush-border membranes.
1987,
Pubmed
Brunette,
Phosphate uptake by superficial and deep nephron brush border membranes. Effect of the dietary phosphate and parathyroid hormone.
1984,
Pubmed
Chong,
Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23.
1993,
Pubmed
Contreras,
Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
1982,
Pubmed
,
Xenbase
Custer,
Localization of NaPi-1, a Na-Pi cotransporter, in rabbit kidney proximal tubules. I. mRNA localization by reverse transcription/polymerase chain reaction.
1993,
Pubmed
,
Xenbase
Delisle,
Immunodetection and characterization of proteins implicated in renal sodium/phosphate cotransport.
1994,
Pubmed
Ghishan,
Characterization and expression of a novel Na(+)-inorganic phosphate transporter at the liver plasma membrane of the rat.
1993,
Pubmed
,
Xenbase
Kos,
Localization of a renal sodium-phosphate cotransporter gene to human chromosome 5q35.
1994,
Pubmed
Magagnin,
Expression cloning of human and rat renal cortex Na/Pi cotransport.
1993,
Pubmed
,
Xenbase
Miyamoto,
Diabetes and glucose transporter gene expression in rat small intestine.
1991,
Pubmed
Miyamoto,
Developmental changes in intestinal glucose transporter mRNA levels.
1992,
Pubmed
Scriver,
X-linked hypophosphataemia: a homologous phenotype in humans and mice with unusual organ-specific gene dosage.
1992,
Pubmed
Sorribas,
Cloning of a Na/Pi cotransporter from opossum kidney cells.
1994,
Pubmed
,
Xenbase
Surin,
Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene.
1984,
Pubmed
Tenenhouse,
Protein kinase activity and protein kinase inhibitor in mouse kidney: effect of the X-linked Hyp mutation and vitamin D status.
1985,
Pubmed
Tenenhouse,
Effect of phosphonoformic acid, dietary phosphate and the Hyp mutation on kinetically distinct phosphate transport processes in mouse kidney.
1989,
Pubmed
Tenenhouse,
Renal Na(+)-phosphate cotransport in murine X-linked hypophosphatemic rickets. Molecular characterization.
1994,
Pubmed
,
Xenbase
Walker,
Presence of multiple sodium-dependent phosphate transport processes in proximal brush-border membrane.
1987,
Pubmed
Wehrle,
Characteristics of phosphate uptake by Ehrlich ascites tumor cells.
1982,
Pubmed
Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
Werner,
Increase of Na/Pi-cotransport encoding mRNA in response to low Pi diet in rat kidney cortex.
1994,
Pubmed
,
Xenbase
