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A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos.
Cole DG
,
Saxton WM
,
Sheehan KB
,
Scholey JM
.
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Pan-kinesin peptide antibodies (Cole, D. G., Cande, W. Z., Baskin, R. J., Skoufias, D. A., Hogan, C. J., and Scholey, J. M. (1992) J. Cell Sci. 101, 291-301; Sawin, K. E., Mitchinson, T. J., and Wordeman, L. G. (1992) J. Cell Sci. 101, 303-313) were used to identify and isolate kinesin-related proteins (KRPs) from Drosophila melanogaster embryonic cytosol. These KRPs cosedimented with microtubules (MTs) polymerized from cytosol treated with AMP-PNP (adenyl-5'-yl imidodiphosphate), and one of them, KRP130, was further purified from ATP eluates of the embryonic MTs. Purified KRP130 behaves as a homotetrameric complex composed of four 130-kDa polypeptide subunits which displays a "slow" plus-end directed motor activity capable of moving single MTs at 0.04 +/- 0.01 microns/s. The 130-kDa subunit of KRP130 was tested for reactivity with monoclonal and polyclonal antibodies that are specific for various members of the kinesin superfamily. Results indicate that the KRP130 subunit is related to Xenopus Eg5 (Sawin, K. E., Le Guellec, K. L., Philippe, M., Mitchinson, T. J. (1992) Nature 359, 540-543), a member of the BimC subfamily of kinesins. Therefore, KRP130 appears to be the first Drosophila KRP, and the first member of the BimC subfamily in any organism, to be purified from native tissue as a multimeric motor complex.
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988, Pubmed
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988,
Pubmed
Brady,
A novel brain ATPase with properties expected for the fast axonal transport motor.
,
Pubmed
Buster,
Purification and assay of kinesin from sea urchin eggs and early embryos.
1991,
Pubmed
Cohn,
Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
1989,
Pubmed
Cole,
Novel heterotrimeric kinesin-related protein purified from sea urchin eggs.
1993,
Pubmed
,
Xenbase
Cole,
Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
1992,
Pubmed
,
Xenbase
Endow,
Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin.
1990,
Pubmed
Endow,
A multimember kinesin gene family in Drosophila.
1991,
Pubmed
Enos,
Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans.
1990,
Pubmed
Gauger,
The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain.
1993,
Pubmed
Gilbert,
Expression, purification, and characterization of the Drosophila kinesin motor domain produced in Escherichia coli.
1993,
Pubmed
Hackney,
Characterization of alpha 2 beta 2 and alpha 2 forms of kinesin.
1991,
Pubmed
Hagan,
Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast.
1992,
Pubmed
Hagan,
Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene.
1990,
Pubmed
Heck,
The kinesin-like protein KLP61F is essential for mitosis in Drosophila.
1993,
Pubmed
Hirokawa,
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
1989,
Pubmed
Howard,
Assay of microtubule movement driven by single kinesin molecules.
1993,
Pubmed
Howard,
Preparation of marked microtubules for the assay of the polarity of microtubule-based motors by fluorescence microscopy.
1993,
Pubmed
Hoyt,
Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
1992,
Pubmed
Hunt,
Kinesin swivels to permit microtubule movement in any direction.
1993,
Pubmed
Ingold,
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
1988,
Pubmed
,
Xenbase
Kuznetsov,
The quaternary structure of bovine brain kinesin.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Le Guellec,
Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.
1991,
Pubmed
,
Xenbase
McDonald,
Identification and characterization of a gene encoding a kinesin-like protein in Drosophila.
1990,
Pubmed
O'Connell,
Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans.
1993,
Pubmed
Roof,
Kinesin-related proteins required for assembly of the mitotic spindle.
1992,
Pubmed
Saunders,
Kinesin-related proteins required for structural integrity of the mitotic spindle.
1992,
Pubmed
Saunders,
Mitotic spindle pole separation.
1993,
Pubmed
Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
,
Xenbase
Sawin,
Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
1992,
Pubmed
,
Xenbase
Saxton,
Drosophila kinesin: characterization of microtubule motility and ATPase.
1988,
Pubmed
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
,
Pubmed
Scholey,
Identification of globular mechanochemical heads of kinesin.
1989,
Pubmed
Siegel,
Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.
1966,
Pubmed
Skoufias,
Cytoplasmic microtubule-based motor proteins.
1993,
Pubmed
Skoufias,
The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
1994,
Pubmed
Stewart,
Identification and partial characterization of six members of the kinesin superfamily in Drosophila.
1991,
Pubmed
Vale,
Microtubule motors: many new models off the assembly line.
1992,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Williams,
Preparation of tubulin from brain.
1982,
Pubmed
Yang,
Evidence that the head of kinesin is sufficient for force generation and motility in vitro.
1990,
Pubmed
Yang,
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
1989,
Pubmed
Yang,
Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.
1988,
Pubmed
Zhang,
A kinesin-like protein required for distributive chromosome segregation in Drosophila.
1990,
Pubmed
de Cuevas,
Evidence that the stalk of Drosophila kinesin heavy chain is an alpha-helical coiled coil.
1992,
Pubmed
