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Purification of a calcium dependent ribonuclease from Xenopus laevis.
Seidel CW
,
Peck LJ
.
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We have purified a Ca2+ dependent ribonuclease from the oocytes of Xenopus leavis. Two properties of this ribonuclease set it apart from other known nucleases. First, Ca2+ was required for ribonuclease activity, and Mg2+ would not substitute. Second, the enzyme specifically degraded RNA and digestion of double or single stranded DNA was not observed. Ca2+ dependent ribonuclease activity of the purified 36-kDa protein was directly observed after renaturation of the protein following electrophoresis in an SDS-Laemmli gel. In addition, the enzyme was shown to have endoribonuclease activity at numerous sites. The Ca2+ dependence suggests that the ribonuclease activity may be modulated by changes in the level of intracellular Ca2+ and thereby provide a direct link to signal transduction systems.
ALEXANDER,
The purification and properties of micrococcal nuclease.
1961, Pubmed
ALEXANDER,
The purification and properties of micrococcal nuclease.
1961,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Deutscher,
Ribonuclease multiplicity, diversity, and complexity.
1993,
Pubmed
Giebelhaus,
Changes in the expression of alpha-fodrin during embryonic development of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Krieg,
Improved synthesis of full-length RNA probe at reduced incubation temperatures.
1990,
Pubmed
Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed
Kubota,
Free calcium wave upon activation in Xenopus eggs.
1987,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Liu,
Ca2+-stimulated ribonucleases from rat mammary gland and R3230AC mammary adenocarcinoma nuclei.
1977,
Pubmed
Mohun,
Temporal and tissue-specific expression of the proto-oncogene c-fos during development in Xenopus laevis.
1989,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Nuccitelli,
The sperm-induced Ca2+ wave following fertilization of the Xenopus egg requires the production of Ins(1, 4, 5)P3.
1993,
Pubmed
,
Xenbase
Oakley,
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
1980,
Pubmed
Rosenthal,
Nuclease detection in SDS-polyacrylamide gel electrophoresis.
1977,
Pubmed
Sachs,
Messenger RNA degradation in eukaryotes.
1993,
Pubmed
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Strauss,
A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
1984,
Pubmed
Vriz,
Differential expression of two Xenopus c-myc proto-oncogenes during development.
1989,
Pubmed
,
Xenbase
Wickens,
In the beginning is the end: regulation of poly(A) addition and removal during early development.
1990,
Pubmed
Young,
A class III transcription factor composed of RNA.
1991,
Pubmed
