Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
An3 mRNA encodes an RNA helicase that colocalizes with nucleoli in Xenopus oocytes in a stage-specific manner.
Gururajan R
,
Mathews L
,
Longo FJ
,
Weeks DL
.
???displayArticle.abstract??? An3 is a maternal mRNA localized to the animal hemisphere of oocytes and early embryos. We have analyzed the enzymatic activity and the subcellular localization of the protein encoded by An3 mRNA during Xenopus oogenesis. Antibodies raised using recombinant full-length and truncated An3 protein recognized a single protein in Xenopus and single proteins from HeLa cells, Drosophila, mouse testes, and Saccharomyces cerevisiae. An3 protein immunoprecipitated from stage IV and stage VI oocytes had ATP-dependent RNA helicase activity. The subcellular location of An3 protein changed during oocyte development. In previtellogenic oocytes, An3 was present throughout the nucleus; cytoplasmic localization was relatively sparse. Nuclear localization in mid-vitellogenic oocytes was primarily nucleolar; cytoplasmic staining increased relative to earlier stages. In stage VI oocytes, An3 protein was detected only in the cytoplasm. The temporal change in An3 protein localization is consistent with a role in the production of large maternal pools of rRNA during oogenesis.
Caizergues-Ferrer,
Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis.
1991, Pubmed,
Xenbase
Caizergues-Ferrer,
Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Gururajan,
The Xenopus localized messenger RNA An3 may encode an ATP-dependent RNA helicase.
1991,
Pubmed
,
Xenbase
Iggo,
Nuclear protein p68 is an RNA-dependent ATPase.
1989,
Pubmed
Jamieson,
A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase.
1991,
Pubmed
Jaramillo,
RNA unwinding in translation: assembly of helicase complex intermediates comprising eukaryotic initiation factors eIF-4F and eIF-4B.
1991,
Pubmed
Lee,
Human RNA helicase A is homologous to the maleless protein of Drosophila.
1993,
Pubmed
Linnen,
Two related localized mRNAs from Xenopus laevis encode ubiquitin-like fusion proteins.
1993,
Pubmed
,
Xenbase
Mosquera,
A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain.
1993,
Pubmed
,
Xenbase
Otte,
Expression and potential functions of G-protein alpha subunits in embryos of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Pause,
The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis.
1993,
Pubmed
Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase
Ripmaster,
A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.
1992,
Pubmed
Rosenberg,
Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
1987,
Pubmed
Sachs,
Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46.
1990,
Pubmed
Schmid,
D-E-A-D protein family of putative RNA helicases.
1992,
Pubmed
Schwartz,
A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.
1992,
Pubmed
,
Xenbase
Schwer,
PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.
1991,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Smith,
Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation.
1988,
Pubmed
,
Xenbase
Thomsen,
Processed Vg1 protein is an axial mesoderm inducer in Xenopus.
1993,
Pubmed
,
Xenbase
Wassarman,
RNA splicing. Alive with DEAD proteins.
1991,
Pubmed
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Weeks,
A maternal mRNA localized to the animal pole of Xenopus eggs encodes a subunit of mitochondrial ATPase.
1987,
Pubmed
,
Xenbase
Wu,
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
1991,
Pubmed
,
Xenbase
