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EMBO J
1994 Mar 01;135:1145-56. doi: 10.1002/j.1460-2075.1994.tb06363.x.
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IDX-1: a new homeodomain transcription factor expressed in rat pancreatic islets and duodenum that transactivates the somatostatin gene.
Miller CP
,
McGehee RE
,
Habener JF
.
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We describe the cloning from a rat islet somatostatin-producing cell line of a 1.4 kb cDNA encoding a new homeoprotein, IDX-1 (islet/duodenum homeobox-1), with close sequence similarity to the Drosophila melanogaster homeobox protein Antennapedia (Antp) and the Xenopus laevis endoderm-specific homeoprotein XlHbox8. Analyses of IDX-1 mRNA and protein in rat tissues show that IDX-1 is expressed in pancreatic islets and ducts and in the duodenum. In electrophoretic mobility shift assays IDX-1 binds to three sites in the 5' flanking region of the rat somatostatin gene. In co-transfection experiments IDX-1 transactivates reporter constructs containing somatostatin promoter sequences, and mutation of the IDX-1 binding sites attenuates transactivation. Reverse transcription-polymerase chain reaction of islet RNA using degenerate amplimers for mRNAs encoding homeoproteins indicates that IDX-1 is the most abundant of 12 different Antp-like homeodomain mRNAs expressed in adult rat islets. The pattern of expression, relative abundance and transcriptional regulatory activity suggests that IDX-1 may be involved in the regulation of islet hormone genes and in cellular differentiation in the endocrine pancreas and the duodenum.
Alpert,
Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons.
1988, Pubmed
Alpert,
Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons.
1988,
Pubmed
Baron,
Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex.
1987,
Pubmed
Bellmann,
Zmhox1a, the product of a novel maize homeobox gene, interacts with the Shrunken 26 bp feedback control element.
1992,
Pubmed
Blumberg,
Organizer-specific homeobox genes in Xenopus laevis embryos.
1991,
Pubmed
,
Xenbase
Boam,
A tissue-specific nuclear factor binds to multiple sites in the human insulin-gene enhancer.
1989,
Pubmed
Bürglin,
Caenorhabditis elegans has scores of homoeobox-containing genes.
1989,
Pubmed
Chick,
A transplantable insulinoma in the rat.
1977,
Pubmed
Cleveland,
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
1980,
Pubmed
Desplan,
The sequence specificity of homeodomain-DNA interaction.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dudek,
Induction of islet cytodifferentiation by fetal mesenchyme in adult pancreatic ductal epithelium.
1991,
Pubmed
Efrat,
Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene.
1988,
Pubmed
Ekker,
Optimal DNA sequence recognition by the Ultrabithorax homeodomain of Drosophila.
1991,
Pubmed
Emens,
Hepatocyte nuclear factor 1 alpha is expressed in a hamster insulinoma line and transactivates the rat insulin I gene.
1992,
Pubmed
Furukubo-Tokunaga,
In vivo analysis of the helix-turn-helix motif of the fushi tarazu homeo domain of Drosophila melanogaster.
1992,
Pubmed
Gamer,
Murine Cdx-4 bears striking similarities to the Drosophila caudal gene in its homeodomain sequence and early expression pattern.
1993,
Pubmed
Gehring,
Homeo boxes in the study of development.
1987,
Pubmed
German,
The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical beta-cell nuclear complexes.
1992,
Pubmed
German,
Synergistic activation of the insulin gene by a LIM-homeo domain protein and a basic helix-loop-helix protein: building a functional insulin minienhancer complex.
1992,
Pubmed
Gorski,
Tissue-specific in vitro transcription from the mouse albumin promoter.
1986,
Pubmed
Green,
Gastrointestinal peptides.
1989,
Pubmed
Han,
Selective expression of rat pancreatic genes during embryonic development.
1986,
Pubmed
Holland,
Expression of homeo box genes during mouse development: a review.
1988,
Pubmed
James,
Homeobox gene expression in the intestinal epithelium of adult mice.
1991,
Pubmed
Kissinger,
Crystal structure of an engrailed homeodomain-DNA complex at 2.8 A resolution: a framework for understanding homeodomain-DNA interactions.
1990,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lacy,
Method for the isolation of intact islets of Langerhans from the rat pancreas.
1967,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated subdomains.
1990,
Pubmed
Levine,
Homeobox genes are expressed in the retina and brain of adult goldfish.
1993,
Pubmed
,
Xenbase
Lin,
Expression of T cell antigen receptor heterodimers in a lipid-linked form.
1990,
Pubmed
Mermod,
The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
1989,
Pubmed
Murtha,
Detection of homeobox genes in development and evolution.
1991,
Pubmed
Nazarali,
Hox-1.11 and Hox-4.9 homeobox genes.
1992,
Pubmed
Nohno,
Involvement of the Chox-4 chicken homeobox genes in determination of anteroposterior axial polarity during limb development.
1991,
Pubmed
Ohlsson,
Novel insulin promoter- and enhancer-binding proteins that discriminate between pancreatic alpha- and beta-cells.
1991,
Pubmed
Orci,
Macro- and micro-domains in the endocrine pancreas.
1982,
Pubmed
Otting,
Protein--DNA contacts in the structure of a homeodomain--DNA complex determined by nuclear magnetic resonance spectroscopy in solution.
1990,
Pubmed
Patel,
Molecular cloning of a homeobox transcription factor from adult aortic smooth muscle.
1992,
Pubmed
Philippe,
Multipotential phenotypic expression of genes encoding peptide hormones in rat insulinoma cell lines.
1987,
Pubmed
Powers,
Somatostatin gene expression in pancreatic islet cells is directed by cell-specific DNA control elements and DNA-binding proteins.
1989,
Pubmed
Prost,
CAT vectors for analysis of eukaryotic promoters and enhancers.
1986,
Pubmed
Renucci,
Comparison of mouse and human HOX-4 complexes defines conserved sequences involved in the regulation of Hox-4.4.
1992,
Pubmed
Ron,
An inducible 50-kilodalton NF kappa B-like protein and a constitutive protein both bind the acute-phase response element of the angiotensinogen gene.
1990,
Pubmed
Schummer,
HOM/HOX homeobox genes are present in hydra (Chlorohydra viridissima) and are differentially expressed during regeneration.
1992,
Pubmed
Scott,
The structure and function of the homeodomain.
1989,
Pubmed
Singh,
Identification of 10 murine homeobox genes.
1991,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Sun,
An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers.
1991,
Pubmed
Takaki,
Isolation of glucagon-secreting cell lines by cloning insulinoma cells.
1986,
Pubmed
Teitelman,
Cell lineage analysis of pancreatic islet development: glucagon and insulin cells arise from catecholaminergic precursors present in the pancreatic duct.
1987,
Pubmed
Vallejo,
Somatostatin gene transcription regulated by a bipartite pancreatic islet D-cell-specific enhancer coupled synergetically to a cAMP response element.
1992,
Pubmed
Vallejo,
Somatostatin gene upstream enhancer element activated by a protein complex consisting of CREB, Isl-1-like, and alpha-CBF-like transcription factors.
1992,
Pubmed
Wedeen,
Evidence for a new family of evolutionarily conserved homeobox genes.
1990,
Pubmed
,
Xenbase
Wessells,
Ultrastructural studies of early morphogenesis and cytodifferentiation in the embryonic mammalian pancreas.
1968,
Pubmed
Yoshinari,
Ontogenetic appearance of immunoreactive endocrine cells in rat pancreatic islets.
1982,
Pubmed
