Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-21573
J Mol Biol 1994 Feb 25;2363:685-90. doi: 10.1006/jmbi.1994.1180.
Show Gene links Show Anatomy links

Nucleosome structural changes due to acetylation.

Bauer WR , Hayes JJ , White JH , Wolffe AP .


???displayArticle.abstract???
We have measured the helical repeat of acetylated nucleosomes reconstituted with HeLa cell histone octamers and the 5 S RNA gene positioning sequence from Xenopus borealis. The hydroxyl radical footprinting experiments show that the helical repeat of nucleosome-bound DNA is unchanged upon acetylation, and that the extent of DNA-protein contacts remains unaltered. These results, combined with previous determinations of the linking number change in minichromosomes as a function of the extent of acetylation, are analyzed with surface linking theory. We demonstrate that the shape of the nucleosome changes upon acetylation, in such a way as to decrease the number of times the DNA winds around the nucleosome central axis.

???displayArticle.pubmedLink??? 8114086
???displayArticle.link??? J Mol Biol