Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Tissue Res
1993 Dec 01;2743:627-30. doi: 10.1007/bf00314561.
Show Gene links
Show Anatomy links
Immunocytochemical identification of growth hormone (GH) cells in the pituitary of three anuran species using an antiserum against purified bullfrog GH.
Olivereau M
,
Olivereau JM
,
Yamashita K
,
Matsuda K
,
Kikuyama S
.
???displayArticle.abstract???
An antiserum was prepared against the recently purified bullfrog (bf) growth hormone (GH); it was applied to sections of brain and pituitary of three urodele (Ambystoma, Pleurodeles and Cynops) and three anuran (Xenopus, Bufo vulgaris and B. japonicus) species. No immunostaining was obtained in the urodele pituitary, being consistent with the results of immunoblot analysis of the pituitary homogenate. In the three anuran species, strong immunoreactivity was observed in GH cells that were concentrated in the posterodorsal region of the pars distalis. No GH-like immunoreactivity was detectable in the brain of any of the species. A comparison using adjacent sections stained with anti-bf prolactin (PRL) confirmed the anteroventral localization of PRL cells. Colocalization of GH and PRL was not apparent. These data suggest that the molecular structure of amphibian GHs is considerably different between anurans and urodeles. The antiserum used in the present work shows a high species specificity, recognizing only anuran GHs. In contrast anti-bfPRL labeled PRL cells in all the amphibian species studied in the present work, suggesting that PRLs possess common amino acid sequences recognized by the anti-bfPRL.
Andersen,
Localization and characterization of prolactin-like immunoreactivity in the pituitary of the frog Rana ridibunda.
1989, Pubmed
Andersen,
Localization and characterization of prolactin-like immunoreactivity in the pituitary of the frog Rana ridibunda.
1989,
Pubmed
Campantico,
Immunocytochemical identification of growth hormone- and prolactin-producing cells in the hypophysis of the newt Triturus cristatus carnifex Laur.
1992,
Pubmed
Frawley,
Mammosomatotropes: presence and functions in normal and neoplastic pituitary tissue.
1991,
Pubmed
Hansen,
Immunohistochemical localization and identification of the prolactin and growth hormone producing cells in the pituitary gland of the toad Bufo bufo bufo (L).
1976,
Pubmed
Hauser-Gunsbourg,
Identification by immunofluorescence of prolactin- and somatotropin-producing cells in the pituitary gland of the Mexican axolotl (Ambystoma mexicanum, Shaw).
1978,
Pubmed
Kobayashi,
Homologous radioimmunoassay for bullfrog growth hormone.
1991,
Pubmed
,
Xenbase
Kobayashi,
Purification and characterization of bullfrog growth hormone.
1989,
Pubmed
,
Xenbase
Olivereau,
[Hypophyseal and thyroidal modifications in Pleurodeles in saline].
1977,
Pubmed
Olivereau,
[Cyto-immunological study of the Pleurodele pituitary gland].
1976,
Pubmed
Sasaki,
Two types of mammosomatotropes in mouse adenohypophysis.
1989,
Pubmed
Schubert,
Histological, immuno- and enzyme-histochemical investigations on the adenohypophysis of the urodeles, Mertensiella caucasica and Triturus cristatus and the caecilian, Chthonerpeton indistinctum.
1977,
Pubmed
Thompson,
Immunocytochemical localization of prolactin in pars distalis of the salamander Necturus maculosus.
1975,
Pubmed
Thorpe,
Immunocytochemical and morphometric studies of mammotrophs, somatotrophs and somatomammotrophs in sheep pituitary cell cultures.
1991,
Pubmed
Yon,
Distribution and characterization of immunoreactive growth hormone (GH) in the pituitary of the frog Rana ridibunda using an antiserum against purified bullfrog GH.
1991,
Pubmed