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EMBO J
1993 Dec 01;1212:4533-45. doi: 10.1002/j.1460-2075.1993.tb06142.x.
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Disruption of the nucleosomes at the replication fork.
Gruss C
,
Wu J
,
Koller T
,
Sogo JM
.
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The fate of parental nucleosomes during chromatin replication was studied in vitro using in vitro assembled chromatin containing the whole SV40 genome as well as salt-treated and native SV40 minichromosomes. In vitro assembled minichromosomes were able to replicate efficiently in vitro, when the DNA was preincubated with T-antigen, a cytosolic S100 extract and three deoxynucleoside triphosphates prior to chromatin assembly, indicating that the origin has to be free of nucleosomes for replication initiation. The chromatin structure of the newly synthesized daughter strands in replicating molecules was analysed by psoralen cross-linking of the DNA and by micrococcal nuclease digestion. A 5- and 10-fold excess of protein-free competitor DNA present during minichromosome replication traps the segregating histones. In opposition to published data this suggests that the parental histones remain only loosely or not attached to the DNA in the region of the replication fork. Replication in the putative absence of free histones shows that a subnucleosomal particle is randomly assembled on the daughter strands. The data are compatible with the formation of a H3/H4 tetramer complex under these conditions, supporting the notion that under physiological conditions nucleosome core assembly on the newly synthesized daughter strands occurs by the binding of H2A/H2B dimers to a H3/H4 tetramer complex.
Bonne-Andrea,
In vitro replication through nucleosomes without histone displacement.
1990, Pubmed
Bonne-Andrea,
In vitro replication through nucleosomes without histone displacement.
1990,
Pubmed
Boyer,
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
1969,
Pubmed
Cech,
Chromatin structure in living cells.
1978,
Pubmed
Cheng,
Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro.
1989,
Pubmed
Cheng,
Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16.
1992,
Pubmed
Clark,
A nucleosome core is transferred out of the path of a transcribing polymerase.
1992,
Pubmed
Conconi,
Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle.
1989,
Pubmed
Conconi,
Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin.
1984,
Pubmed
Cusick,
Dispersive segregation of nucleosomes during replication of simian virus 40 chromosomes.
1984,
Pubmed
Fotedar,
Multistep pathway for replication-dependent nucleosome assembly.
1989,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Gruss,
Nucleosome assembly in mammalian cell extracts before and after DNA replication.
1990,
Pubmed
Gruss,
Chromatin replication.
1992,
Pubmed
,
Xenbase
Guo,
Specific transcription factors stimulate simian virus 40 and polyomavirus origins of DNA replication.
1992,
Pubmed
Guo,
Origin auxiliary sequences can facilitate initiation of simian virus 40 DNA replication in vitro as they do in vivo.
1989,
Pubmed
Hanson,
Cross-linking of DNA in situ as a probe for chromatin structure.
1976,
Pubmed
Hoang,
The replication activation potential of selected RNA polymerase II promoter elements at the simian virus 40 origin.
1992,
Pubmed
Ishimi,
Preincubation of T antigen with DNA overcomes repression of SV40 DNA replication by nucleosome assembly.
1992,
Pubmed
Jackson,
Deposition of newly synthesized histones: misinterpretations due to cross-linking density-labeled proteins with Lomant's reagent.
1987,
Pubmed
Jackson,
In vivo studies on the dynamics of histone-DNA interaction: evidence for nucleosome dissolution during replication and transcription and a low level of dissolution independent of both.
1990,
Pubmed
Jakobovits,
A nucleosome-free region in SV40 minichromosomes.
1980,
Pubmed
Klempnauer,
Maturation of newly replicated chromatin of simian virus 40 and its host cell.
1980,
Pubmed
Koller,
High resolution physical mapping of specific binding sites of Escherichia coli RNA polymerase on the DNA of bacteriophage T7 .
1978,
Pubmed
Krude,
Transfer of nucleosomes from parental to replicated chromatin.
1991,
Pubmed
Krude,
A nucleosome assembly factor is a constituent of simian virus 40 minichromosomes.
1993,
Pubmed
Lanford,
Expression of simian virus 40 T antigen in insect cells using a baculovirus expression vector.
1988,
Pubmed
Li,
Simian virus 40 DNA replication in vitro.
1984,
Pubmed
Lusky,
Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
1981,
Pubmed
Peden,
Isolation of mutants of an animal virus in bacteria.
1980,
Pubmed
Randall,
The fate of parental nucleosomes during SV40 DNA replication.
1992,
Pubmed
Rodríguez-Campos,
Assembly and properties of chromatin containing histone H1.
1989,
Pubmed
,
Xenbase
Sapp,
Purification and mechanism of action of a nucleosome assembly factor from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Saragosti,
Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
1980,
Pubmed
Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Shimamura,
The assembly of regularly spaced nucleosomes in the Xenopus oocyte S-150 extract is accompanied by deacetylation of histone H4.
1989,
Pubmed
,
Xenbase
Simanis,
An immunoaffinity purification procedure for SV40 large T antigen.
1985,
Pubmed
Smith,
Stepwise assembly of chromatin during DNA replication in vitro.
1991,
Pubmed
Sogo,
Electron microscopy of chromatin.
1989,
Pubmed
Sogo,
Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin.
1984,
Pubmed
Sogo,
Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.
1986,
Pubmed
Stillman,
Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
1985,
Pubmed
Sugasawa,
Nonconservative segregation of parental nucleosomes during simian virus 40 chromosome replication in vitro.
1992,
Pubmed
Wolffe,
Implications of DNA replication for eukaryotic gene expression.
1991,
Pubmed
Worcel,
Assembly of newly replicated chromatin.
1978,
Pubmed
Yang,
Roles of DNA topoisomerases in simian virus 40 DNA replication in vitro.
1987,
Pubmed
Zucker,
The histone H3/H4.N1 complex supplemented with histone H2A-H2B dimers and DNA topoisomerase I forms nucleosomes on circular DNA under physiological conditions.
1990,
Pubmed
,
Xenbase
