Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Primary sequence and functional expression of a novel beta subunit of the P-ATPase gene family.
Jaisser F
,
Horisberger JD
,
Rossier BC
.
???displayArticle.abstract???
The cortical collecting tubule (CCT) of the mammalian kidney reabsorbs sodium and potassium, processes that are mediated by Na/K-ATPase and H/K-ATPase. CCT is also an important site for proton secretion, which is driven, in part, by H/K-ATPase. Na/K-ATPase and H/K-ATPase are members of the ion-motive P-ATPase gene family. They are closely related plasma membrane proteins which consist of alpha beta heterodimers. The urinary bladder of the toad Bufo marinus is the amphibian counterpart of mammalian CCT. We have previously characterized a ouabain-resistant Na/K-ATPase [see ref. 17], from TBM cells, a clonal cell line derived from the toad bladder, which expresses transepithelial sodium transport. In the present study, we report the primary sequence and functional expression of a novel beta subunit (beta bladder = beta b1) isolated from a toad bladderepithelial cell cDNA library. The deduced polypeptide is 299 amino acids in length and has a predicted molecular mass of 33 kDa. The beta b1 protein exhibits 35% amino acid identity to the previously characterized beta 1 of B. marinus Na/K-ATPase and 39% identity with beta 3 of B. marinus Na/K-ATPase. It shares 38% identity with the mammalian beta gastric H/K-ATPase and 52% with the mammalian beta 2 Na/K-ATPase. Northern blot analysis shows that a 1.4 x 10(3)-base mRNA is expressed at a high level in bladder epithelial cells and eye and at a trace level in kidney; it is not detectable in significant amounts in the stomach, colon and small intestine.(ABSTRACT TRUNCATED AT 250 WORDS)
Canessa,
Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase alpha subunit confers ouabain resistance.
1992, Pubmed,
Xenbase
Canessa,
Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase alpha subunit confers ouabain resistance.
1992,
Pubmed
,
Xenbase
Cheval,
K(+)-ATPase-mediated Rb+ transport in rat collecting tubule: modulation during K+ deprivation.
1991,
Pubmed
Crowson,
Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus.
1992,
Pubmed
Del Castillo,
Apical membrane localization of ouabain-sensitive K(+)-activated ATPase activities in rat distal colon.
1991,
Pubmed
Doucet,
Characterization of K-ATPase activity in distal nephron: stimulation by potassium depletion.
1987,
Pubmed
Eakle,
High-affinity ouabain binding by yeast cells expressing Na+, K(+)-ATPase alpha subunits and the gastric H+, K(+)-ATPase beta subunit.
1992,
Pubmed
Erlij,
Urinary Ca2+ and the regulation of K+ secretion in toad bladder by neurohypophyseal hormones.
1992,
Pubmed
Geering,
The functional role of the beta-subunit in the maturation and intracellular transport of Na,K-ATPase.
1991,
Pubmed
,
Xenbase
Ghosh,
Expression of multiple Na+,K(+)-ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: functional implications in aqueous humor secretion.
1991,
Pubmed
Gifford,
H(+)-K(+)-ATPase activity in rat collecting duct segments.
1992,
Pubmed
Gloor,
The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase.
1990,
Pubmed
Gonda,
Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues.
1982,
Pubmed
Good,
A nervous system-specific isotype of the beta subunit of Na+,K(+)-ATPase expressed during early development of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Horisberger,
Coexpression of alpha 1 with putative beta 3 subunits results in functional Na+/K+ pumps in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Horisberger,
The H,K-ATPase beta-subunit can act as a surrogate for the beta-subunit of Na,K-pumps.
1991,
Pubmed
,
Xenbase
Horisberger,
Structure-function relationship of Na,K-ATPase.
1991,
Pubmed
Jaisser,
A putative H(+)-K(+)-ATPase is selectively expressed in surface epithelial cells of rat distal colon.
1993,
Pubmed
Jaisser,
Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump.
1992,
Pubmed
,
Xenbase
Jaisser,
Mechanisms of urinary K+ and H+ excretion: primary structure and functional expression of a novel H,K-ATPase.
1993,
Pubmed
,
Xenbase
Kaunitz,
Identification of a vanadate-sensitive potassium-dependent proton pump from rabbit colon.
1986,
Pubmed
Kirley,
Determination of three disulfide bonds and one free sulfhydryl in the beta subunit of (Na,K)-ATPase.
1989,
Pubmed
Kraehenbuhl,
Microfilament-rich cells in the toad bladder epithelium.
1979,
Pubmed
Ludens,
Acidification of urine by the isolated urinary bladder of the toad.
1972,
Pubmed
Ma,
cDNA cloning of the beta-subunit of the human gastric H,K-ATPase.
1991,
Pubmed
Martin-Vasallo,
Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression.
1989,
Pubmed
Pandiyan,
Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium-depleted rat distal colon.
1992,
Pubmed
Planelles,
Biochemical and functional characterization of H(+)-K(+)-ATPase in distal amphibian nephron.
1991,
Pubmed
Rabon,
The mechanism and structure of the gastric H,K-ATPase.
1990,
Pubmed
Renaud,
Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport.
1991,
Pubmed
Reuben,
Characterization of a beta subunit of the gastric H+/K(+)-transporting ATPase.
1990,
Pubmed
Schmalzing,
The adhesion molecule on glia (AMOG/beta 2) and alpha 1 subunits assemble to functional sodium pumps in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Shull,
cDNA cloning of the beta-subunit of the rat gastric H,K-ATPase.
1990,
Pubmed
Shyjan,
The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity.
1990,
Pubmed
Shyjan,
Evolution of the Na,K- and H,K-ATPase beta subunit gene family: structure of the murine Na,K-ATPase beta 2 subunit gene.
1991,
Pubmed
Suzuki,
Ouabain-sensitive H+-K+ exchange mechanism in the apical membrane of guinea pig colon.
1989,
Pubmed
Tabuchi,
Ouabain-insensitive, vanadate-sensitive K(+)-ATPase of rat distal colon is partly similar to gastric H+,K(+)-ATPase.
1992,
Pubmed
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
Wallmark,
Inhibition of gastric H+,K+-ATPase and acid secretion by SCH 28080, a substituted pyridyl(1,2a)imidazole.
1987,
Pubmed
Wallmark,
Evidence for acid-induced transformation of omeprazole into an active inhibitor of (H+ + K+)-ATPase within the parietal cell.
1984,
Pubmed
Watanabe,
Ouabain-sensitive K(+)-ATPase in epithelial cells from guinea pig distal colon.
1990,
Pubmed