XB-ART-22368
Mol Biol Cell
1993 Aug 01;48:781-90.
Show Gene links
Show Anatomy links
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
Shibuya EK
,
Ruderman JV
.
???displayArticle.abstract???
Mitogen-activated protein kinases (MAPKs) are rapidly and transiently activated when both quiescent Go-arrested cells and G2-arrested oocytes are stimulated to reenter the cell cycle. We previously developed a cell-free system from lysates of quiescent Xenopus oocytes that responds to oncogenic H-ras protein by activating a MAPK, p42MAPK. Here, we show that the oncogenic protein kinase mos is also a potent activator of p42MAPK in these lysates. Mos also induces p42MAPK activation in lysates of activated eggs taken at a time when neither mos nor p42MAPK is normally active, showing that the mos-responsive MAPK activation pathway persists beyond the stage where mos normally functions. Similarly, lysates of somatic cells (rabbit reticulocytes) also retain a mos-inducible MAPK activation pathway. The mos-induced activation of MAPKs in all three lysates leads to phosphorylation of the pp90rsk proteins, downstream targets of the MAPK signaling pathway in vivo. The in vitro activation of MAPKs by mos in cell-free systems derived from oocytes and somatic cells suggests that mos contributes to oncogenic transformation by inappropriately inducing the activation of MAPKs.
???displayArticle.pubmedLink??? 8241566
???displayArticle.pmcLink??? PMC300992
???displayArticle.link??? Mol Biol Cell
???displayArticle.grants??? [+]
Species referenced: Xenopus
Genes referenced: gnao1 hras mapk1 mos
References [+] :
Ahn,
Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
1991, Pubmed
Ahn, Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase. 1991, Pubmed
Alcorta, Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase. 1989, Pubmed , Xenbase
Allende, Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors. 1988, Pubmed , Xenbase
Anderson, Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase. 1990, Pubmed
Bar-Sagi, Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. 1985, Pubmed
Barbacid, ras genes. 1987, Pubmed
Bercovich, Degradation of ornithine decarboxylase in reticulocyte lysate is ATP-dependent but ubiquitin-independent. 1989, Pubmed
Birchmeier, ras proteins can induce meiosis in Xenopus oocytes. 1985, Pubmed , Xenbase
Bollag, Regulators and effectors of ras proteins. 1991, Pubmed
Boulton, Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies. 1991, Pubmed
Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 1976, Pubmed
Chen, Identification of Xenopus S6 protein kinase homologs (pp90rsk) in somatic cells: phosphorylation and activation during initiation of cell proliferation. 1990, Pubmed , Xenbase
Ciechanover, Degradation of nuclear oncoproteins by the ubiquitin system in vitro. 1991, Pubmed
Cobb, Extracellular signal-regulated kinases: ERKs in progress. 1991, Pubmed
Crews, Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product. 1992, Pubmed
Crews, The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. 1992, Pubmed
Daar, A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells. 1991, Pubmed , Xenbase
Dent, Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro. 1992, Pubmed
Druker, Oncogenes, growth factors, and signal transduction. 1989, Pubmed
Drury, Effects of cycloheximide on the "autocatalytic" nature of the maturation promoting factor (MPF) in oocytes of Xenopus laevis. 1975, Pubmed , Xenbase
Dumont, Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. 1972, Pubmed , Xenbase
Elion, Functional redundancy in the yeast cell cycle: FUS3 and KSS1 have both overlapping and unique functions. 1991, Pubmed
Erikson, In vivo phosphorylation and activation of ribosomal protein S6 kinases during Xenopus oocyte maturation. 1989, Pubmed , Xenbase
Erikson, Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6. 1991, Pubmed , Xenbase
Erikson, Purification and characterization of a protein kinase from Xenopus eggs highly specific for ribosomal protein S6. 1986, Pubmed , Xenbase
Erikson, Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts. 1987, Pubmed , Xenbase
Ferrell, Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs. 1991, Pubmed , Xenbase
Freeman, Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes. 1989, Pubmed , Xenbase
Freeman, Phosphorylation of conserved serine residues does not regulate the ability of mosxe protein kinase to induce oocyte maturation or function as cytostatic factor. 1992, Pubmed , Xenbase
Freeman, Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle. 1991, Pubmed
Gotoh, Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF. 1991, Pubmed , Xenbase
Gotoh, In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase. 1991, Pubmed , Xenbase
Gómez, Dissection of the protein kinase cascade by which nerve growth factor activates MAP kinases. 1991, Pubmed
Hattori, Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system. 1992, Pubmed , Xenbase
Howe, Activation of the MAP kinase pathway by the protein kinase raf. 1992, Pubmed
Jackson, Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA. 1983, Pubmed
Jones, A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases. 1988, Pubmed , Xenbase
Kanki, Progression from meiosis I to meiosis II in Xenopus oocytes requires de novo translation of the mosxe protooncogene. 1991, Pubmed , Xenbase
Kosako, cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates. 1993, Pubmed , Xenbase
Kosako, Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation. 1992, Pubmed , Xenbase
Krohne, Proteins of pore complex--lamina structures from nuclei and nuclear membranes. 1983, Pubmed , Xenbase
Kurata, Dose-dependent regulation of macrophage differentiation by mos mRNA in a human monocytic cell line. 1989, Pubmed
Kurata, Production of recombinant rat viruses as a method of oncogene isolation in coculture medium. 1987, Pubmed
Kyriakis, Raf-1 activates MAP kinase-kinase. 1992, Pubmed
Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 1970, Pubmed
Lodish, Different lifetimes of reticulocyte messenger RNA. 1976, Pubmed
Lohka, Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. 1985, Pubmed , Xenbase
Masui, Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens. 1967, Pubmed
Murray, Cyclin synthesis drives the early embryonic cell cycle. 1989, Pubmed , Xenbase
Nakielny, MAP kinase activator from insulin-stimulated skeletal muscle is a protein threonine/tyrosine kinase. 1992, Pubmed
Nebreda, The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs. 1993, Pubmed , Xenbase
Nishizawa, The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes. 1992, Pubmed , Xenbase
Noda, Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. , Pubmed
O'Keefe, Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg. 1989, Pubmed
Okazaki, Differential occurrence of CSF-like activity and transforming activity of Mos during the cell cycle in fibroblasts. 1992, Pubmed , Xenbase
Paules, Mouse Mos protooncogene product is present and functions during oogenesis. 1989, Pubmed , Xenbase
Pomerance, Stimulation of mitogen-activated protein kinase by oncogenic Ras p21 in Xenopus oocytes. Requirement for Ras p21-GTPase-activating protein interaction. 1992, Pubmed , Xenbase
Posada, Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts. 1991, Pubmed , Xenbase
Posada, Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes. 1992, Pubmed , Xenbase
Posada, Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro. 1993, Pubmed , Xenbase
Posada, Molecular signal integration. Interplay between serine, threonine, and tyrosine phosphorylation. 1992, Pubmed
Rapp, Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. 1983, Pubmed
Ruderman, MAP kinase and the activation of quiescent cells. 1993, Pubmed
Sagata, Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes. 1988, Pubmed , Xenbase
Sagata, The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation. 1989, Pubmed , Xenbase
Sagata, The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs. 1989, Pubmed , Xenbase
Sanghera, Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes. 1990, Pubmed
Scheffner, The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. 1990, Pubmed
Seger, Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. 1992, Pubmed
Shibuya, Activation of p42 MAP kinase and the release of oocytes from cell cycle arrest. 1992, Pubmed
Shibuya, Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes. 1992, Pubmed , Xenbase
Thomas, Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinases. 1992, Pubmed
Wasserman, Effects of cyclohexamide on a cytoplasmic factor initiating meiotic naturation in Xenopus oocytes. 1975, Pubmed , Xenbase
Wasserman, A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization. 1976, Pubmed , Xenbase
Watanabe, Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs. 1989, Pubmed , Xenbase
Watanabe, Independent inactivation of MPF and cytostatic factor (Mos) upon fertilization of Xenopus eggs. 1991, Pubmed , Xenbase
Wood, ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK. 1992, Pubmed
Wu, Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase. 1993, Pubmed
Yew, Meiotic initiation by the mos protein in Xenopus. 1992, Pubmed , Xenbase