Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1993 Jul 15;9014:6596-600.
Show Gene links
Show Anatomy links
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.
Dascal N
,
Lim NF
,
Schreibmayer W
,
Wang W
,
Davidson N
,
Lester HA
.
???displayArticle.abstract???
Injection of rat atrial RNA into Xenopus oocytes resulted in the expression of guanine nucleotide binding (G) protein-activated K+ channel. Current through the channel could be activated by acetylcholine or, if RNA encoding a neuronal 5HT1A receptor was coinjected with atrial RNA, by serotonin (5HT). A 5HT-evoked current (I5HT) was observed in oocytes injected with ventricle RNA fractions (of 2.5-5.5 kb) and 5HT1A receptor RNA. I5HT displayed strong inward rectification with very little conductance above the K+ equilibrium potential, was highly selective for K+ over Na+, and was blocked by 5-300 microM Ba2+. I5HT was suppressed by intracellular injection of the nonhydrolyzable analog of GDP, guanosine 5'-[beta-thio]diphosphate, but not by treatment with pertussis toxin (PTX), suggesting coupling of the receptor to the G-protein-activated K+ channel via a PTX-insensitive G protein, possibly endogenously present in the oocyte. Coexpression of the alpha subunit of a PTX-sensitive G protein, G(i2), rendered I5HT sensitive to PTX inhibition. Native oocytes displayed a constitutively active inwardly rectifying K+ current with a lower sensitivity to Ba2+ block; expression of a similar current was also directed by atrial or ventricle RNA of 1.5-3 kb. Xenopus oocytes may be employed for cloning of the G-protein-activated K+ channel cDNA and for studying the coupling between this channel and G proteins.
Aldrich,
Potassium channels. Advent of a new family.
1993, Pubmed,
Xenbase
Aldrich,
Potassium channels. Advent of a new family.
1993,
Pubmed
,
Xenbase
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Andrade,
A G protein couples serotonin and GABAB receptors to the same channels in hippocampus.
1986,
Pubmed
Andrade,
Pharmacologically distinct actions of serotonin on single pyramidal neurones of the rat hippocampus recorded in vitro.
1987,
Pubmed
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
Breitwieser,
Uncoupling of cardiac muscarinic and beta-adrenergic receptors from ion channels by a guanine nucleotide analogue.
,
Pubmed
Brown,
Ionic channels and their regulation by G protein subunits.
1990,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Ciani,
A model for anomalous rectification: electrochemical-potential-dependent gating of membrane channels.
1978,
Pubmed
Codina,
The alpha subunit of the GTP binding protein Gk opens atrial potassium channels.
1987,
Pubmed
Codina,
Hormonal regulation of pituitary GH3 cell K+ channels by Gk is mediated by its alpha-subunit.
1987,
Pubmed
Dascal,
Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
1984,
Pubmed
,
Xenbase
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Friel,
Dual control by ATP and acetylcholine of inwardly rectifying K+ channels in bovine atrial cells.
1990,
Pubmed
Gilman,
G proteins: transducers of receptor-generated signals.
1987,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Horie,
Rectification of muscarinic K+ current by magnesium ion in guinea pig atrial cells.
1987,
Pubmed
Hoyer,
5-HT receptors: subtypes and second messengers.
1991,
Pubmed
Iijima,
Membrane currents and their modification by acetylcholine in isolated single atrial cells of the guinea-pig.
1985,
Pubmed
Ito,
On the mechanism of G protein beta gamma subunit activation of the muscarinic K+ channel in guinea pig atrial cell membrane. Comparison with the ATP-sensitive K+ channel.
1992,
Pubmed
Karschin,
Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.
1991,
Pubmed
Kim,
G-protein beta gamma-subunits activate the cardiac muscarinic K+-channel via phospholipase A2.
1989,
Pubmed
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Kurachi,
G protein activation of cardiac muscarinic K+ channels.
1992,
Pubmed
Kurachi,
Acetylcholine activation of K+ channels in cell-free membrane of atrial cells.
1986,
Pubmed
Kurachi,
On the mechanism of activation of muscarinic K+ channels by adenosine in isolated atrial cells: involvement of GTP-binding proteins.
1986,
Pubmed
Kurachi,
Activation of atrial muscarinic K+ channels by low concentrations of beta gamma subunits of rat brain G protein.
1989,
Pubmed
Kurachi,
Alpha-adrenergic activation of the muscarinic K+ channel is mediated by arachidonic acid metabolites.
1989,
Pubmed
Lewis,
Expression of an inwardly rectifying K+ channel from rat basophilic leukemia cell mRNA in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed
Lübbert,
Rat brain 5-HT1C receptors are encoded by a 5-6 kbase mRNA size class and are functionally expressed in injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Miledi,
Effects of defolliculation on membrane current responses of Xenopus oocytes.
1989,
Pubmed
,
Xenbase
North,
Mu and delta receptors belong to a family of receptors that are coupled to potassium channels.
1987,
Pubmed
Olate,
Molecular cloning and sequence determination of four different cDNA species coding for alpha-subunits of G proteins from Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Pfaffinger,
GTP-binding proteins couple cardiac muscarinic receptors to a K channel.
,
Pubmed
Périer,
Expression of an inwardly rectifying potassium channel in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Sakmann,
Acetylcholine activation of single muscarinic K+ channels in isolated pacemaker cells of the mammalian heart.
,
Pubmed
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Silver,
Intrinsic gating of inward rectifier in bovine pulmonary artery endothelial cells in the presence or absence of internal Mg2+.
1990,
Pubmed
VanDongen,
Newly identified brain potassium channels gated by the guanine nucleotide binding protein Go.
1988,
Pubmed
Yatani,
Direct activation of mammalian atrial muscarinic potassium channels by GTP regulatory protein Gk.
1987,
Pubmed
Yatani,
The G protein-gated atrial K+ channel is stimulated by three distinct Gi alpha-subunits.
1988,
Pubmed
