Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
An RNA polymerase chain reaction strategy was used to amplify and clone a cDNA segment encoding for the complete constant part of the axolotl IgY heavy (C upsilon) chain. C upsilon is 433 amino acids long and organized into four domains (C upsilon 1-C upsilon 4); each has the typical internal disulfide bond and invariant tryptophane residues. Axolotl C upsilon is most closely related to Xenopus C upsilon (40% identical amino acid residues) and C upsilon 1 shares 46.4% amino acid residues among these species. The presence of additional cysteines in C upsilon 1 and C upsilon 2 domains is consistent with an additional intradomain S-S bond similar to that suggested for Xenopus C upsilon and C chi, and for the avian C upsilon and the human C epsilon. C upsilon 4 ends with the Gly-Lys dipeptide characteristic of secreted mammalian C gamma 3, human C epsilon 4, and avian and anuran C upsilon 4, and contains the consensus [G/GT(AA)] nucleotide splice signal sequence for joining C upsilon 4 to the transmembrane region. These results are consistent with the hypothesis of an ancestral structural relationship between amphibian, avian upsilon chains, and mammalian epsilon chains. However, these molecules have different biological properties: axolotl IgY is secretory Ig, anuran and avian IgY behave like mammalian IgG, and mammalian IgE is implicated in anaphylactic reactions.
Amemiya,
Nucleotide sequence of a cDNA encoding a third distinct Xenopus immunoglobulin heavy chain isotype.
1989, Pubmed,
Xenbase
Amemiya,
Nucleotide sequence of a cDNA encoding a third distinct Xenopus immunoglobulin heavy chain isotype.
1989,
Pubmed
,
Xenbase
Bernstein,
Complete sequence of a cloned cDNA encoding rabbit secreted mu-chain of VHa2 allotype: comparisons with VHa1 and membrane mu sequences.
1984,
Pubmed
Dahan,
Nucleotide sequence of the constant region of a chicken mu heavy chain immunoglobulin mRNA.
1983,
Pubmed
Fellah,
Transient developmental expression of IgY and secretory component like protein in the gut of the axolotl (Ambystoma mexicanum).
1992,
Pubmed
Fellah,
Ontogeny of immunoglobulin expression in the Mexican axolotl.
1989,
Pubmed
Fellah,
Characterization of an IgY-like low molecular weight immunoglobulin class in the Mexican axolotl.
1988,
Pubmed
Fellah,
Evolution of vertebrate IgM: complete amino acid sequence of the constant region of Ambystoma mexicanum mu chain deduced from cDNA sequence.
1992,
Pubmed
,
Xenbase
Feng,
Progressive sequence alignment as a prerequisite to correct phylogenetic trees.
1987,
Pubmed
Flanagan,
Mechanisms of divergence and convergence of the human immunoglobulin alpha 1 and alpha 2 constant region gene sequences.
1984,
Pubmed
Flanagan,
The sequence of a human immunoglobulin epsilon heavy chain constant region gene, and evidence for three non-allelic genes.
1982,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Grey,
Duck immunoglobulins. II. Biologic and immunochemical studies.
1967,
Pubmed
Hadji-Azimi,
Anuran immunoglobulins. A review.
1979,
Pubmed
,
Xenbase
Harding,
Two distinct immunoglobulin heavy chain isotypes in a primitive, cartilaginous fish, Raja erinacea.
1990,
Pubmed
Hayzer,
cDNA clones encoding rabbit immunoglobulin lambda chains. Evidence for length variation of the third hypervariable region and for a novel constant region.
1987,
Pubmed
Hellman,
Characterization and molecular cloning of the mRNA for the heavy (epsilon) chain of rat immunoglobulin E.
1982,
Pubmed
Hsu,
A third immunoglobulin class in amphibians.
1985,
Pubmed
,
Xenbase
Kobayashi,
Identification of a second immunoglobulin in the most primitive shark, the frill shark, Chlamydoselachus anguineus.
1992,
Pubmed
Kokubu,
Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate.
1988,
Pubmed
Leslie,
Phylogeny of immunoglobulin structure and function. VI. 17S, 7.5S and 5.7S anti-DNP of the turtle, Pseudamys scripta.
1972,
Pubmed
Litman,
N-terminal amino-acid sequence of African lungfish immunoglobulin light chains.
1971,
Pubmed
Liu,
Cloning and nucleotide sequence of mouse immunoglobulin epsilon chain cDNA.
1982,
Pubmed
Lobb,
Phylogeny of immunoglobulin in structure and function-x. Humoral immunoglobulins of the sheepshead, Archosargus probatocephalus.
1981,
Pubmed
Magor,
Structural relationship between the two IgY of the duck, Anas platyrhynchos: molecular genetic evidence.
1992,
Pubmed
Mansikka,
Chicken IgA H chains. Implications concerning the evolution of H chain genes.
1992,
Pubmed
Marchalonis,
Isolation and characterization of immunoglobulin-like proteins of the Australian lungfish (Neoceratodus forsteri).
1969,
Pubmed
Martens,
Molecular genetic analysis of genes encoding the heavy chains of rabbit IgG.
1984,
Pubmed
Meyer,
Origin of tetrapods inferred from their mitochondrial DNA affiliation to lungfish.
1990,
Pubmed
Parvari,
Chicken immunoglobulin gamma-heavy chains: limited VH gene repertoire, combinatorial diversification by D gene segments and evolution of the heavy chain locus.
1988,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Reynaud,
Complete sequence of a chicken lambda light chain immunoglobulin derived from the nucleotide sequence of its mRNA.
1983,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schluter,
Evolution of immunoglobulin light chains: cDNA clones specifying sandbar shark constant regions.
1989,
Pubmed
Schwager,
The ontogeny of diversification at the immunoglobulin heavy chain locus in Xenopus.
1991,
Pubmed
,
Xenbase
Schwager,
Amino acid sequence of heavy chain from Xenopus laevis IgM deduced from cDNA sequence: implications for evolution of immunoglobulin domains.
1988,
Pubmed
,
Xenbase
Seno,
Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA.
1983,
Pubmed
Shamblott,
Complete nucleotide sequence of primitive vertebrate immunoglobulin light chain genes.
1989,
Pubmed
Symons,
Structure of bovine immunoglobulin constant region heavy chain gamma 1 and gamma 2 genes.
1989,
Pubmed
Word,
The human immunoglobulin C mu-C delta locus: complete nucleotide sequence and structural analysis.
1989,
Pubmed